分离

To dissect single cells promising to culture from tissues, we researched the time of hydrolyzation of the tissues by tryposin, the result showed that the optimum time of hydrolyzation of the tissues was 5~10min when pretreated with 0.25% tryposin under the optimum conditions of pH8.0 at 37 C.We cultured four tissues: embryo at mediat-anaphase, head of high-age nymphae, spermary and ovary of adult.

组织块分离出来后，为了能获得单个的、有培养前景的细胞，本实验又研究了低浓度的胰蛋白酶在其最适温度和最适pH条件下，获得分离程度适宜的组织块的不同酶解时间，结果0.25％的胰蛋白酶在37℃、pH8.0的条件下酶解组织块的最适时间是5～10min。

It is derived that the optimal temperature,optimal pH value,optimal carbon content in media and concentration of NaCl for the growth of oligotrophic bacteria are 37 ℃,8～9,15 mg/L and 1%～20% respectively,which can provide the new conception and theoretical foundation for researching the microorganisms,inhabiting under the peculiar environmental,especially the oligotrophic microorganisms.

通过对新疆荒漠地区分离得到的五株低营养细菌的理化特性研究，初步摸索到了分离培养的方法，并测得其生长温度范围和最适温度37℃；最适pH值8～9；最适含碳量15 mg/L培养基，生长需要1%～20%的NaCl浓度，以及一系列生理生化反应指标，为特殊环境微生物尤其是低营养微生物的研究提供了一些新的思路和理论依据。

The effective isolate 2507 producing antibiotic controlling oomycetes was first isolated from the soil samples.

本研究首先是进行有效菌株分离筛选工作，首次从世界各地采集的土样中分离筛选菌株，抗生素生产菌株2507可以防治植物卵菌病害。

Based on relative literatures,this part has included five resesearch aspects as below.1 Fifteen compounds were isolated and purified by extraction,column chromatography,and their structures were determined on the basis of spectral analysis: acteoside(Ⅰ-1),isoacteoside(Ⅰ-2),crenatoside(Ⅰ-3),cistanoside F(Ⅰ-4),sinapoyl-4-O-β-D-glucoside(Ⅰ-5),adenosine(Ⅰ-6),β-siterol(Ⅰ-7),oleanic acid(Ⅰ-8), succinic acid(Ⅰ-9),caffeic acid(Ⅰ-10),protocatechuic aldehyde(Ⅰ-11),p-hydro xybenzyl alcohol(Ⅰ-12),β-daucosterol(Ⅰ-13),D-galacitol(Ⅰ-14),D-mannitol(Ⅰ-15).Ⅰ-4～15 were obtained from this plant for the first time,andⅠ-6,7,9,and 13 were isolated from Orobanche genus for the first time.2 The scavenging test of DPPH showed that most compounds have comparative antioxidant activity as L-ascorbic acid and part of them show better activity such as the O.coerulescens extract and phenylethanoid glycosides.Acteoside showed potent free radical scavenging effects with a median inhibition concentration of 25.6μg/ml.3 The anti-HBV activities of acteoside,isoacteoside and crenatoside were measured,and all of them showed suppressive activity on the expression of HBsAg and HBeAg in the HepG2.2.15 cell line.

本论文在文献调研基础上对紫花列当化学成分及生物活性进行了研究，并从免疫抗病毒角度探讨紫花列当中特征性成分类叶升麻苷的肝保护作用及其机制。1采用大孔树脂、硅胶和Sephadex LH-20等色谱技术对紫花列当进行系统的植物化学研究，从中分离得到19个化合物，利用UV和NMR等波谱手段及理化性质鉴定了其中的15个化合物，分别为类叶升麻苷(Ⅰ-1)、异类叶升麻苷(Ⅰ-2)、crenatoside(Ⅰ-3)、cistanoside F(Ⅰ-4)、sinapoyl-4-O-β-D-glucoside(Ⅰ-5)、腺苷(Ⅰ-6)、β-谷甾醇(Ⅰ-7)、齐墩果酸(Ⅰ-8)、琥珀酸(Ⅰ-9)、咖啡酸(Ⅰ-10)、原儿茶醛(Ⅰ-11)、对羟甲基苯甲酸(Ⅰ-12)、β-胡萝卜苷(Ⅰ-13)、D-半乳糖醇(Ⅰ-14)和甘露醇(Ⅰ-15除化合物Ⅰ-1、Ⅰ-2和Ⅰ-3外，其余化合物均为首次从该植物中分离得到。2)通过大孔树脂富集该药材有效部位苯乙醇总苷，并采用HPLC测得其所含特征性成分类叶升麻苷的含量可达80%以上。3DPPH自由基清除试验显示紫花列当提取物及其所含的苯乙醇苷类化合物均具有较好的抗氧化能力，其清除DPPH自由基能力接近于抗坏血酸。4采用卡介苗整体致敏、脂多糖离体攻击构建大鼠原代肝细胞免疫性损伤模型，在体外观察类叶升麻苷的保肝作用。

Methods:rThe protein from persons'ovarial carcinoma tissue and normal ovary was drawn,and the spots of protein were identified with the Swiss-Prot database.

取人卵巢正常及恶性肿瘤组织，提取蛋白质，进行双向电泳分离，银染获得其蛋白质电泳分离图谱对蛋白质斑点进行初步鉴定。

Secondly, resorting to the relative gradient, it is shown that the natural gradient learning algorithm for overdetermined BSS has the same form as that for usual complete BSS, which is verified by simulation results.

此后，借助于相对梯度的概念，证明超定盲信号分离与通常的完备盲信号分离具有相同形式的自然梯度算法。仿真试验验证了算法的有效性。

Resorting to the relative gradient, we show that the natural gradient algorithm for overdetermined BSS has the same form as that for usual complete BSS.

然后，借助于相对梯度严格证明，完备盲信号分离的自然梯度算法同样适用于超定盲信号分离问题。

A white needle crystal was isolated and purified on systematic extraction of the alkaloid by silica gel column and gradient sublimation from Oxytropis glabra.

摘要采用生物堿系统提取、矽胶柱层析分离及梯度升华技术从小花棘豆干草粉中分离出一种白色针状结晶。

The compound 1 was isolsted from the genus Paeonia for the first time. The compounds 2, 3 were isolated from this plant for the first time.

化合物1为从该属中首次分离得到，化合物2，3为从该植物首次中分离得到。

Methods: hMSCs were separated from human marrow with Ficoll Paque reagent or direct culture and expanded in different culture medium with newborn bovine serum. The proliferation and growth characteristics were observed in primary and passage culture.

采用Ficoll Paque淋巴细胞分离液和全血接种两种方法分离成人MSC，筛选体外培养hMSC适合的培养基和适宜的血清含量，流式细胞仪检测hMSC表面抗原表达。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。