分离

In comparison with the pure standards and the retention indices RI(subscript s reported in the literature, a total of 302 volatile compounds were identified in Changyu XO brandy, including 30 alcohols, 35 aldehydes and ketones, 20 carboxylic acids, 104 esters, 24 substituted benzenes and derivatives, 14 phenolic derivatives, 14 acetals, 16 furan derivatives, 22 terpenic and norisoprenoidic derivatives and 23 others.

实验先采用液－液萃取方法提取张裕XO级白兰地的挥发性成分，然后将酸性成分与碱性和中性成分分离，再采用柱色谱分离手段将其分离为若干个级分并浓缩，采用气相色谱-质谱、标准品比对、保留指数值比较等方法对分离得到的各级分中的成分进行了鉴定，在白兰地中共鉴定出302种挥发性成分，包括醇30种、醛酮类35种、酸类20种、酯类104种、苯同系物及其衍生物24种、酚类14种、缩醛14种、呋喃类16种、萜烯类22种和其他物质23种。

The neutral/basic fraction was furthermore separated into 4 fractions using silica gel normal phase chromatography, and each fraction was then concentrated and analyzed using gas chromatography-mass spectrometry. In comparison with the pure standards and the retention indices reported in the literature, a total of 302 volatile compounds were identified in Changyu XO brandy, including 30 alcohols, 35 aldehydes and ketones, 20 carboxylic acids, 104 esters, 24 substituted benzenes and derivatives, 14 phenolic derivatives, 14 acetals, 16 furan derivatives, 22 terpenic and norisoprenoidic derivatives and 23 others.

实验先采用液-液萃取方法提取张裕XO级白兰地的挥发性成分，然后将酸性成分与碱性和中性成分分离，再采用柱色谱分离手段将其分离为若干个级分并浓缩，采用气相色谱-质谱、标准品比对、保留指数值比较等方法对分离得到的各级分中的成分进行了鉴定，在白兰地中共鉴定出302种挥发性成分，包括醇30种、醛酮类35种、酸类20种、酯类104种、苯同系物及其衍生物24种、酚类14种、缩醛14种、呋喃类16种、萜烯类22种和其他物质23种。

Considering the resemblance between paper chromatography and electrophoresis on distinguishing mixture of different monosaccharides or amino acids and eluting of interest component,we have a try to separate the components of some amylases which have been hydrolyzed by paper chromatography before polyacrylamide gel electrophoresis.

考虑到纸层析技术与电泳技术在分离物质(都可以对糖类混合物或者氨基酸混合物进行分离)和后期处理(都可以将含有目标成分的部分进行洗脱)电泳技术的相似处，本文在使用电泳分离出F_1-ATP合酶之前，先对水解后的多糖进行了尝试性的纸层析分离。

objective to establish immunological methods specific for detecting antigens in different groups of monoclonal antibodies.methods indirect immnofluorescence assay was applied to identify specificity of the two groups of monoclonal antibodies prepared with crude antigen and recombinant antigen of aspergillus fumigatus,respectively.two different double monoclonal antibody sandwich elisa assays established with the two groups of antibodies were performed to detect antigents in the cell culture supermatants of 19 common species of aspergillus,penicillium marneffei,and 5 species of candidas.results the results of indirect immnofluorescence assay indicated that the monoclonal antibodies prepared with crude antigen of aspergillus fumigatus were specific for antigens in both clinical isolates and environmental isolates of aspergillus, whereas the other group of monoclonal antibodies was proved to be specific for aspergillus fumigatus of both clinical and environmental isolates.the elisa assay established with the crude antigen-specfic monoclonal antibodies could detect both of the clinical and environmental isolates of aspergllius, while the other assay could only detect aspergillus fumigatus of both clinical and environmental isolates.and no cross reaction with the cell culture of penialllium marneffei and candidas was observed with the two methods.conclusion the elisa assays can detect both of the clinical and environmental isolates of aspergillus,and differentiate aspergillus fumigatus from other species of aspergillus.

目的 用2组曲霉单克隆抗体建立特异性识别不同种类曲霉抗原的检测方法。方法采用天然烟曲霉抗原免疫，获得广谱针对曲霉抗原的单克隆抗体；采用重组烟曲霉抗原获得特异性针对烟曲霉抗原的单克隆抗体，用间接免疫荧光鉴定，并分别建立2种双抗体夹心elisa法，对19种常见的环境和临床分离曲霉株、马尔尼菲氏青霉菌及念珠菌培养液进行检测。结果间接免疫荧光显示，用天然烟曲霉抗原免疫获得的单克隆抗体(mabs-1)可广谱识别多种曲霉分离株，而重组烟曲霉抗原获得的单克降抗体(mabs-2)仅能特异性结合临床和环境分离的烟曲霉抗原。用mabs-1建立的双抗体夹心elisa法可检测19种常见曲霉株培养液；用特异性针对烟曲霉抗原单克降抗体(mabs-2)建立的双抗体夹心elisa法可特异性检测临床和环境分离株烟曲霉培养液；与其他曲霉株无交叉反应；2种双抗体夹心elisa法与马尔尼菲氏青霉菌及念珠菌培养液均无交叉反应。

Methods PBMCs from 10 heparinized blood specimens of HIV-1 infected persons were collected to isolate the virus with PBMC cocultivation method. Neuramindase was added to the culture medium to raise the HIV isolation rate. The supernatant of cocultures were detected by p24 antigen capture assay and also IFA test was used to identify the isolates. Cell tropism of the HIV-1 isolates was tested byuse of H9 and MT4 cell lines, respectively.

采集10份福建艾滋病病毒感染者肝素抗凝血，分离外周血单核细胞，与健康人PBMC共培养进行HIV-1的分离，使用含神经氨酸酶的T细胞培养液提高病毒分离率，通过检测P24抗原、间接免疫荧光试验及电镜观察等确定病原分离结果。

The relationship between field of view and departure maneuver is developed.(2) The departure trajectory and safety characteristic are discussed for V-bar departure, R-bar departure and arbitrary direction departure.

1研究了视场约束下V-bar撤离的径向和切向冲量方案，利用解析和数值结合的方法得到了冲量大小和视场角的关系；(2)针对V-bar分离、R-bar分离和任意方向分离，分别研究了分离后的相对轨迹及被动安全特性。

All of these provide scientific basis for development of the novel cyclone. At last, according to the thorough study of gas-solid process, The cyclone shape and size are improved, and the cyclone performances are taken better. The technique of cyclone with down-flow is updated, and applied in CFB boilers, retrofitting old boilers, CFB pulverized-coal gasifier, flue gas cleaner, and so on. The successful operation of 75T/h CFB boiler indicts that the novel technique tends to ripe.

最后，根据对气固分离过程的深入研究，对下排气旋风分离器进行了结构改进与性能完善，实现了下排气旋风分离技术的升级换代，并将技术成果成功应用于下排气循环床锅炉、旧锅炉改造、流化床煤粉气化炉及烟气除尘净化等方面。75T/h下排气循环床锅炉的成功运行表明下排气旋风分离技术已趋成熟，发展前景广阔。

Based on studies about current development of Methods of separating meiofauna, a new method is advanced in this paper. A suit of device was designed to Separate meiofauna from benthic sediments sample automatically, accurately and efficiently, which based on the technology of Laser-induced Fluorescence Detection, and used other technologies, such as noctilucence, electronics, biophysics, computer, mechanism, and so on. The scheme of macrocosm, the design of light route, the hardware and software design of the photoelectricity detection system and machine control system, the method of signal processing are introduced, especially the hardware and software design and the method of signal processing.

论文分析了目前国内外所使用的小型底栖生物分离方法现状，并以此为基础，自主开发了一套基于激光诱导荧光技术，并且集荧光化学技术、电子物理技术、光电测量技术、计算机技术及机械技术为一体的小型底栖生物标本自动分离系统，实现了从沉积物样品中自动分离小型底栖生物标本，从而大幅度提高室内小型底栖生物分样的工作效率和精度的目的，并以此完成了大洋协会&十五&攻关项目：&小型底栖生物标本自动分离系统研制&。

A planar-type optical isolator includes a substrate, a first mode splitter, a second mode splitter and a phase shift region formed on the substrate between the first mode splitter and the second mode splitter.

一种平面型光隔离器包括基底，第一模式分离器，第二模式分离器和形成在第一模式分离器和第二模式分离器之间的基底上的相移区。

Subsequently W2, W3, F1, F2 and F4 strains were isolated. The average isolation rate was 50% with the highest rate of 62% from lamella parts and the lowest rate of 38% from tissues between lamellae and pilei.

并对采集到的10株松口蘑子实体，分离出W2、W3、F1、F2和F4五个菌株，平均分离成功率为50%，其中，菌褶分离成功率为62%，菌褶与菌盖之间菌肉组织分离成功率为38%。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。