分离

Isolation and culture of osteoclasts: Osteoclasts were isolated and cultured from long bone cavities of human aborticide fetus.

一、破骨细胞的分离、培养：采用机械法，从引产胎儿的长骨骨腔中分离出破骨细胞，进行培养。

Isotope separation ; stable isotope ; multi-component mixture separation ; cascade theory ; matched abundance ratio cascade

同位素分离；稳定同位素；多组分分离；级联理论；相对丰度匹配级联

The chloroform phase was isolated into 7 phases by column chromatograpathy, and the bioassy showed that the 1 phase has strong inhibitory effect, was 91.01% on Abutilon theophrasti M., root length, 51.35%、63.93%on Echinochloa crusgalli L, seedling length and root length, and the Amaranthus retroflexux L.,germination rate is 0, the secondly was 2 phase and 5 phas, and next we continued iaolated these two phases.

用硅胶柱对氯仿组分进行分离得到7个组分，生物测定表明组分1化感作用最强，对苘麻根长抑制率为91.01%，对稗草芽长和根长的抑制率分别为51.35%、63.93%，反枝苋的发芽率为0；其次是组分2和组分5，由于组分2的含量相对较少，对组分1和组分5继续分离。

HPLC was coupled with both mass spectroscopy and ultraviolet absorption spectroscopy for simultaneous separation and characterization of the products from glow plasma reaction of chloroform.(3) Phosphorus pentachloride has been used to perchlorinate acenaphthene under moderate pressure and temperature in sealed reaction vessels.

在此基础上，以反相C〓柱为固定相，甲醇/乙醇/环己烷为流动相，采用梯度洗脱的方法，对氯仿辉光放电合成产物进行高效液相色谱-紫外光谱-质谱联用分离分析，成功的分离得到包括富勒烯C〓和全氯代碳簇在内的数十种产物，并得到了相应的质谱和光谱数据。

In this study, the CPE processes of four different kinds of polyaromatic hydrocarbons, acenaphthene, anthracene, phenanthrene and pyrene, with two kinds of silicone surfactants, DC-193 and DC-190, were studied separately, and four different assisting methods for the phase separation of the CPE process including heating at a higher temperature, adding salts, centrifugation and stirring were introduced, respectively.

今利用两种聚醚型有机硅表面活性剂DC-193和DC-190，对蒽、菲、芘和苊四种多环芳烃进行浊点萃取研究，分别使用加热、加盐、离心和搅拌等辅助方法改变相分离过程的速度。通过测定表面活性剂富集相的体积百分率Vs/随时间的变化和最终的萃取率，研究上述四种不同辅助方法对相分离行为和萃取性能的影响。

At present, the understanding of ecology of acidophilic microorganisms is mainly based on the traditional pure culture and isolation techniques. However, the development of molecular biology provides more scientific methods. The combination of pure culture and isolation techniques with molecular biotechnology will provide more effective methods in this field.

目前对嗜酸微生物生态学的理解主要基于传统的纯菌培养分离技术，但是分子生物学技术的发展提供了更科学的研究手段，将纯菌培养分离技术和分子生物学技术相结合是研究嗜酸微生物生态的有效方法。

Methods Four pretreatments and 5 media supplemented with different inhibitors were used for isolation.

用不同的样品预处理方式和分离培养基，并添加不同的抑制剂进行分离；根据放线菌的菌落数和出菌率确定最佳分离方法组合。

The Company will carry out reconciliations of records and Segregated Funds with the records and accounts of the money the Company holds in Segregated Accounts on a daily basis, and any required transfer to or from the Segregated Account will take place by the close of business on the day that the reconciliation is performed.

公司将通过日常基础的公司持有分离账户的记录和款项进行记录及有记录的分离账户的和解，而且任何要求的分离账户的转入或转出会在行使和解当天收盘之前进行。

Now that we have added the necessary base methods for detaching the child entities, child entity sets and delay loaded properties, we can complete the Product Detach method by adding a Detach call for the Category, ItemList and Desc property.

现在我们已经增加了对儿童的实体分离的必要基础的方法，儿童实体集和延迟加载的属性，我们可以完成添加的分类，ItemList和实价财产分离调用产品分离的方法。

The isolated cells are able to orient and migrate to the magnet in artificial magnetic field but could not orient swimming only under the geomagnetic field. Magnetosomes produced by Acidithiobacillus ferrooxidans were range from 40nm to 90 nm according to the results of TEM. Energy dispersive X-ray analysis indicated that extracted magnetic particles consisted of oxygen and iron. The results show that some Acidithiobacillus ferrooxidans cells have weak magnetotaxis and they could be able to be separated by solid-plate magnetophoresis method from others. With the development of this new isolation method, it is possible that we could do deeper research to generate a comprehensive description of the mechanism that how Acidithiobacillus ferrooxidans synthesize the magnetic particles.

实验结果表明，氧化亚铁硫杆菌具有较弱的趋磁性，在人工磁场下会进行趋磁运动，但仅在地磁场作用下不能定向运动，利用固体平板磁泳筛选纯化含有磁性颗粒的氧化亚铁硫杆菌的方法是切实可行的，磁泳分离技术的进一步完善和改进为传统的微生物菌种分离提供了新的途径，为研究纯氧化亚铁硫杆菌菌株胞内磁性颗粒的形成条件及机理提供了前提条件，也为今后从浸矿细菌中分离筛选更多的含有磁性颗粒的菌株打下基础。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐