分离

This is the first report of pectenolone and pectenoxanthin, two types of carotenoid, in the muscle of the Yesso scallop.3 Roles of the carotenoids composition and accumulation in Yesso scallopThe method for detecting carotenoids using reverse phase high performance liquid chromatography was developed. The conditions of RP-HPLC analysis were an analytical scale (200×4.6 mm, inner diameter 5μm) Eclipse XDB-C18 column of Agilent corporation; 10μl injection volume; 25°C column temperature; acetonitrile, methanol and methylene dichloride mobile phase (40:56:4, V/VV) with 0.1% BHT; 1.0 ml per minute flow rate; 450 nm UV-VIS detector; 8 minutes elution.The carotenoids from orange muscle of Yesso scallop of 7, 10, 12, 15, 18, 22 months old was detected using the method refered above.

通过柱层析、高效液相等手段对橘红色闭壳肌中的主要类胡萝卜素进行了提取分离，分离提纯得到两种类胡萝卜素，并通过MS、~1H-NMR和~(13)C-NMR对其进行结构鉴定，结果表明(3S，3\'R)-3，3\'-dihydroxy-7\'，8\'-didehydro-β，β-Caroten-4-one和(3S，3\'S)-7，7\'，8，8\'-tetradehydro-β，β-Carotene-3，3\'-diol，俗称pectenolone和pectenoxanthin是橘红色闭壳肌中的主要类胡萝卜素，这是国内外首次对虾夷扇贝闭壳肌中的类胡萝卜素进行提纯及结构鉴定。3虾夷扇贝类胡萝卜素的种类组成和累积变化规律建立了橘红色闭壳肌中类胡萝卜素的HPLC分析定量检测技术：色普柱为Agilent公司的Eclipse XDB-C18柱200mm×4.6mm，i.d.5μm进样体积，5μl ；流速1ml/min；检测波长450nm；柱温25℃；流动相为乙腈：甲醇：二氯甲烷(V/V/V=40：56：4并加入0.1%的BHT；分离时间为8min。

MethodsThe chemical constituents from zygophyllum fabag were isolated using silical gel and Sephedax LH-20 colomn chromatography and structurally identified by means of various modern spectroscopic technologies based upon their physical properties.

方法利用反复硅胶柱层析、Sephadex LH-20 和制备HPLC等手段进行分离，经光谱分析和理化常数进行鉴定。结果从该植物中分离鉴定了7种化学成分，其中6种首次从该植物分离得到。

The working process of set is as follows; the liquid of raw material is pumped in through diaphragm pump. The liquid of raw material atomizes into tiny droplets. Then it gathers with hot air and fall off. Most parts of powder material will be collected through the outlet of discharge at the bottom. The wasted air and fine power will be separated through cyclone.

本机组工作过程为料液通过隔膜高压输入，喷出雾状液滴，然后同热空气并流下降，大部分粉粒由塔底排料口收集，废气及其微小粉末经旋风分离器分离，废气由抽风机排出，粉末由设在旋风分离器下端的收粉筒收集，风机出口处还可以装备二级除尘装置，回收率在96-98%以上。

Simulations show that the algorithm can get high separation performance for both non-stationary temporary and convolved mixing signals.

仿真结果表明算法分离精度高，分离过程平稳，在任意混合情况下均能获得良好的分离性能。

After summarizing BSS algorithm of convolved mixture and its applications, the paper applies the improved PSO algorithm to convolved mixture BSS and proposes the PSO algorithm based on simulated annealing method. The paper also does some experiments of BSS based on PSO algorithm with grads acceleration and analyzes the simulation results.

4在总结卷积混合的盲源分离算法及其应用之后，将改进型粒子群算法应用到卷积混合的盲源分离中，给出了基于模拟退火思想的粒子群算法和基于带有梯度加速的粒子群算法的盲源分离的仿真实验，并对仿真结果作了分析。

Effects of microbial transglutaminase on the tensile properties of plastic made from soy protein isolates are studied. The results show that the tensile strength and Young modulus of the protein plastic are increased from 9.2MPa and 265.4MPa of the control, which is not treated with TG, to 14.7MPa and 390.1Mpa respective, which is treated with TG at 50℃ for 60min. High molecular weight protein conjugates are formed with TG treatment, and they are responsible for the enhanced tensile properties, denaturalizing temperature and enthalpy values.

研究了微生物谷氨酰氨转胺酶对大豆分离蛋白塑料拉伸性能的影响，发现在50℃时转氨酶对大豆分离蛋白催化聚合反应60 min，形成的高分子聚合物使大豆分离蛋白的抗拉强度和杨氏模量分别由未经处理时的9.2 MPa和265.4 MPa增加到14.7 MPa和390.1 MPa，同时增加了蛋白质的变性温度和热焓值。

The broth was centrifuged to wipe off cells, and then the supernatant was desalted and concentrated by hollow fiber membrane.

对表达的重组人胰岛素原的后分离纯化步骤研究得到了优化的分离步骤组合和分离条件。

After Alcalase hydrolysis both peptide fractions were desalted by adsorption on a macroporous resin and then eluted with ethanol at different concentrations.

这两种组分经Sephadex G-15分离时，0.01 mol/L的HCl溶液作为洗脱液对降胆固醇肽的分离有较好的效果；1 mol/L的醋酸溶液作为洗脱液对降血压肽的分离有较好的效果。

The steps were as follows: Acquiring superant from the culture medium by centrifugation, discarding waste residue by filtration, concentrating by hollow-fiber membrane, treating with salting-out, dialyzing the product of salting-out, treating the dialysate by vaccum freeze dryer, dissolving solid , using the method of gel filtration chromatography with Sephadex G-100/G-75, and proof-test by filter-paper chromatography ,then preparation by thin-layer chromatography was carried out.

建立了分离纯化该物质的有效途径：发酵液离心除菌→抽滤除杂→中空纤维过滤浓缩→盐析→透析→真空冷冻干燥→溶解→交联葡聚糖凝胶Sephadex G-100凝胶分离→交联葡聚糖凝胶Sephadex G-75凝胶分离→纸层析检验→薄层层析制备。

Methods:Human embryonic tissues containing genital ridges and dorsal mesenteries were isolated and then cultured in 5 different patterns: simple mechanical disaggregation,trysinization, combination of the former 2 and with/without feeder cells.

体外分离人胚胎生殖嵴和肠系膜组织，按单纯机械分离、酶消化分离、二者结合法及是否有饲养层将组织分为 5种不同的方式培养后，用碱性磷酸酶标记，计数阳性克隆并进行比较。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。