分离

With pulse sampling and based on the difference in electric migration rates of components to be separated, a eluting stream is applied to elute different components sequentically.

随后采用脉冲进样方式，利用待分离组分在凝胶膜中的电迁移速率差异作为分离基础，在与电场方向相垂直的方向上施加一冲洗载流，将从膜中先后移出的组分依次冲出，从而实现分离。

Methods: We detected serum antibody of rattus,hare, wild boar,muntiacus reevesi ogilby, fox, dog and cattle with mCF method. SFGR were isloated from Ixodiodeas with Embryonate hens eggs and identified with mCF and PCR/RFLP.

鼠类、野兔、野猪、麂、狐狸、狗、牛血清学检测，采用微量室温补体结合法；病原分离：用鸡胚卵黄囊感染法分离SFGR，并用 mCF、PCR/RFLP技术对分离株进行鉴定。

In the normal uterus, Cytokeratins immunolabelling were detected in glandular cell, luminal epithelial cell, Vimentin immunolabelling were detected in stromal cell and endoblastic cell; CK7 immunolabelling were not detected in any tissue of the yak utenus.

研究结果显示：未妊娠时，泛角蛋白在子宫内膜腺上皮细胞、腔上皮细胞内表达，波形蛋白在子宫内膜基质细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，牦牛子宫任何部位均不表达角蛋白7；妊娠30天左右时，泛角蛋白在子宫内膜腺上皮细胞、子宫内膜腔上皮细胞、滋养层细胞、内胚层细胞和尿囊细胞内表达，波形蛋白在子宫内膜基质细胞和内胚层细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，角蛋白7在尿囊细胞内表达，偶尔在腔上皮细胞的细胞核边缘表达；消化法进行原代培养时，组织经胶原酶消化并通过100目和400目筛网组合可以有效地分离原代子宫内膜基质细胞和子宫内膜腺上皮细胞；分离得到的子宫内膜基质细胞活率达90%以上，并可在体外传代7次以上；分离得到的子宫内膜腺上皮细胞活率可达85%以上，并可在体外传代5次以上；RPMI1640培养基最适合子宫内膜基质细胞和子宫内膜腺上皮细胞的生长，维持子宫内膜基质细胞正常生长的FBS添加量为20%，维持子宫内膜腺上皮细胞正常生长的胎牛血清添加量为30%。

The invention belongs to the fluid measuring technical field and relates to an oil, gas and water three-phase oil well continuous gauging device, which comprises a high-efficient gas-liquid separator; the high-efficient gas-liquid separator is connected with an oil, gas and water three-phase inflowing port; a gas phase outlet of the oil, gas and water three-phase inflowing port is connected with a gas phase measuring pipeline; a gas path temperature sensor, a gas path pressure sensor and a gas path endocone flowmeter with high-frequency differential pressure transmitter are arranged on the gas phase measuring pipeline; a liquid phase outlet of the high-efficient gas-liquid separator is connected with a liquid phase measuring pipeline; a liquid path Coriolis mass flowmeter and a liquid path endocone flowmeter are arranged on the liquid phase measuring pipeline; and the cross section of the pipeline for mounting the liquid path Coriolis mass flowmeter is more than the cross section of the pipeline for mounting the liquid path endocone flowmeter.

本发明属于流体测量技术领域，涉及一种油、气、水三相油井连续计量装置，包括高效气液分离器，高效气液分离器与油、气、水三相流入口相连，其气相出口与气相测量管路相连，在气相测量管路上装有气路温度传感器、气路压力传感器、带有高频差压变送器的气路内锥流量计；高效气液分离器的液相出口与液相测量管路相连，在液相测量管路上装有液路科氏质量流量计和液路内锥流量计，安装液路科氏质量流量计的管路的截面积大于安装液路内锥流量计的管路的截面积。

The study reveals that Shanxi Province is rich in entomogenous fungi diversity. About 72 species of 17 genera of fungi are believed exist in the province. Among the fungi collection, 63 species are collected from four national nature reserves in the province, 12 from six major forest areas and another 22 species from the area around Taiyuan. This respectively represents 64.9%, 12.4% and 22.7% of the species collection.

结果表明，山西省虫生真菌资源十分丰富， 10年共采集分离到虫生真菌17属72种，在山西省的四大国家级自然保护区、六大林区及太原等县市分别分离到虫生真菌63种、12种和22种，分别占分离到的山西省虫生真菌的64.9%、12.4%和22.7%。

In this research, Delphi language is adopted to work out calculation procedure of coal-pulverizing system, use this procedure to perform checking calculation, and act as the theoretic basis of operating adjustment. The operation characteristics and controllable parameters are completely studied to reduce the cost. The double-stage separating technique is adopted to improve separation efficiency. The pulverized coal exhauster is reformed based on the outcome of resistance calculation to save power consumption of pulverized system. The wearing problem of steel balls is studied, has set up the stable state mathematics model of steel ball wear to certain load ball system and addition ball system.

本课题采用Delphi语言编制了制粉系统计算程序，用此程序对该制粉系统进行了校核计算，作为制粉系统优化调整的理论根据，从中储式制粉系统的运行特性、可控参数方面进行了全方位的研究，采用内筒中增设螺旋隔离分离元件的双级分离式细粉分离技术，提高细粉分离效率，根据制粉系统阻力计算结果对排粉机进行改造，减少制粉电耗。

Based on an analysis of distribution pattern of hydrocyclone"s axial velocity, by changing the inlet flow rate Qi and split ratio Fand making an investigation in the effect of Qi and F on axial velocity field, the factors causing the forming of short-cut circuit and circulating flow are found. It is considered that the short-cut circuit and circulating flow are produced by the structure of the hydrocyclone, and can"t be eliminated completely, though they interfere the flow field inside a hydrocyclone and affect the separation accuracy. Test results show that, by increasing Qi and F and controlling them in a certain range, the separation efficiency of the hydrocyclone can be improved.

在研究旋流器轴向速度分布规律的基础上，分析了短路流与循环流的形成原因，试验研究了改变操作参数，即改变入口流量Qi与分流比F对轴向速度场的影响认为短路流与循环流是由旋流器结构决定的，很难完全消除，它们干扰了旋流腔和大锥段的流场，在一定程度上也影响了分离精度试验结果表明，加大入口流量Qi与分流比F，并将其控制在一定范围内，便可提高分离效率这项研究对水力旋流器分离机理的认识与实际应用均有重要意义

The purpose is to probe into fow characteristic pressure distribution of separator under primitive structure and improved structure.

介绍了旋风分离器的基础理论，包括数学模型，分离效率、阻力损失和临界粒径等旋风分离器的主要参数指标，并给出它们的一些研究现状和计算的经验公式。

Gingerols in ginger oleoresin were separated by AB8 macroporous adsorption resin and silica gel column chromatography. The samples were characterized by the gas chromatography-mass spectrometry method.The peak area normalization method was used to determine the relative percentage of the components and the rate of accumulation of gingerol was determined by contrasting with gingerol standard samples. The results showed that gingerols was concentrated on the 3rd and the 4th parts.

采用硅胶柱层析和AB8大孔树脂吸脱附两种方法分离姜油树脂中的姜酚，采用气相色谱-质谱(gas chromatography-mass spectrometry， GC-MS)对分离样品进行表征，用峰面积归一化法对测试样品的气相色谱-质谱结果进行分析，确定了分离样品中各组分的相对峰面积百分比。

Then the utilization solvent parting method, low pressure silica gel separation method and so on column chromatograph, opposition silica gel, glucosan gelatin chromatograph, pocket resin, carries on the chemical composition to the effective spot the system separation; The chemical composition obtains which to the separation carries on the structure appraisal with four big spectra.

然后运用溶剂分离法、低压硅胶柱色谱、反相硅胶、葡聚糖凝胶色谱、大孔树脂等分离手段，对有效部位进行化学成分的系统分离；对分离得到的化学成分用四大光谱进行结构鉴定。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。