分离

The optimum condition was determined via orthogonal experiments, and the result shows that the technology of bile extraction and purification adopts 10L chromato bar with 3L bile, gel silica as fixed phase, the mixed chloroform and isopropanol as mobile phase, gradient elution with mobile phase (the mobile phase ratio in extraction of cholalic acid and glycocholic acid is 4:1 , and then becomes 3:2 in extraction of glycocholic acid and glycocholic acid), and eluting velocity of 3～5mL/s .

采用正交试验筛选了柱层析最佳条件，以容积为10L的层析柱进行分离纯化，固定相为硅胶，流动相为氯仿和正丁醇混合溶剂，一次装样量3000mL胆汁，流动相采用梯度洗脱，先按4：1比例分离胆酸和甘氨胆酸，然后按3：2比例分离甘氨胆酸和牛磺胆酸，流动相洗脱速度为3～5mL/s。

In the mortality studies, 8 isolates infected more than 80% of the grubs, another 65 isolates infected more than 50% of the grubs and the rest of the isolates had a low level of pathogenicity with 107 conidia/mL concentration.

於致死试验中，有8个分离株感染了超过80%的蛴螬，另有65个分离株感染了超过50%的蛴螬，而其余浓度为10^7 conidia/mL的分离株致病力低。

The main work of this thesis includes three aspects:(1) study the existing order adjustment methods and examine the characteristics of speeches, a new order adjustment method based on energy correlation is proposed. It is applied to JADE, KM, c-FastICA, and CMN to separate the convolutive mixtures from simulated and actual speeches, the results of analysis and comparison of separation performance verify the validity of the method.

针对卷积混合语音盲分离的频域算法，本文主要做了以下三方面工作：(1)研究了现有调序方法和语音信号的特性，提出了一种基于能量相关的调序方法，并应用JADE、KM、c-FastICA和CMN等四种复数BSS算法，对仿真和实际语音信号进行了盲分离，分析比较了分离性能，验证了本文调序方法的有效性。

We deduce an BSS algorithm for time-delay mixture model and an algorithm for the convolutive mixture model under the principal of Maximum Output Entropy, separately. We also provide a comparison between the two separating structures of the convolutive mixture model.

我们在最大输出熵的原则下，分别推导得出了一种时延混合模型的盲分离算法和卷积混合模型的盲分离算法，而且给出了对于卷积混合模型的两种分离结构及两者之间的优缺点。

In order to isolate the active compounds in ethyl acetate fraction of Psoralea corylifolia L., activity-guided isolation was performed along with chromatographic techniques. Four active compounds were isolated from ethyl acetate fraction and identified by their NMR spectral data and physical-chemical properties. They are corylin and bavachin, which showed stimulating effect on osteoblastic proliferation and differentiation, isopsoralen and psoralen exhibited activity of promoting cell differentiation in some degree.

为了追踪分离补骨脂乙酸乙酯萃取物中的活性成分，本实验采用多种色谱分离技术，以活性测试为导向，结合波谱技术及理化数据，从补骨脂乙酸乙酯萃取物中分离并鉴定了4个活性化合物的化学结构，它们是具有促进细胞增殖和分化作用的补骨脂异黄酮和补骨脂二氢黄酮，对细胞分化有一定促进作用的异补骨脂素和补骨脂素。

By simulation, the optimal operation conditions were gained in every step in the separation process, and polymerization-class isoprene was gained by the process. And to improve the economy of process, the process was studied by simulation, 99% cyclopentene and 1, 3-pentadiene were gained by continuous separation process. All were the base of the process industrialization.

通过模拟研究，得到分离各步的最佳塔高，进料位置和各种操作条件；并进行了全流程的模拟优化得到聚合级的异戊二烯的各种工艺参数；通过模拟研究对分离工艺进一步深化，得到了从C5中分离出环戊烯和间戊二烯的优化工艺条件，对工艺经济性进一步优化，给过程工业化提供了依据。

In this paper, based on the fundamental principle of the three-point method roundness error separation technique, the separation of cylindricity error factors and the reconstruction of the measured cylinder profile was intensively studied, and the methods of evaluating the cylindricity form error were discussed. Furthermore, the separation and reconstruction methods of the cylindricity error proposed in this paper were examined by the experiment.

本文采用三点法圆度误差分离技术的基本原理，重点研究圆柱度误差要素的分离和被测圆柱体形貌重构问题，探讨圆柱度形状误差的评定方法，并对本文所提的圆柱度误差分离及重构方法进行实验验证。

The cylindricity could be calculated by least squares method and wavelet error separation method that the measurement error could be decomposed by Harmonic analysis algorithm based on wavelet theory.

针对激光 CCD 灵敏度高，干扰噪声容易混入测量数据中的问题，提出了一种基于小波分析的误差分离方法，即运用谐波分析的思想方法和小波理论中多频率分析算法进行误差分离，误差分离后的数据用最小二乘法处理求出圆柱度。

In anticipation of achieving the above objectives, marine-derived actinomycetes, H2003 and Aspergillus terreus Thom have been screened by using this model, the EtOAC extracts of strain H2003 and Aspergillus terreus Thom showed bioactivities of inhibiting the cell cycle progression and cytoclasis of t...

并采用活性追踪分离方法对一株放线菌和一株真菌的代谢产物活性成分进行了初步研究采用减压硅胶柱层析、凝胶柱层析(Sephadex LH-20)、反相硅胶柱层析和反相制备高效液相等分离手段，从具有细胞坏死活性的放线菌H2003分离得到22个单体化合物。

In the rice leaves infected by RDV Yunnan isolate, the distribution of cells full of virus particles formed a belt which was a center of infection and multiplication of virus, around the cells fall of virus particles were some cells with the different extent of lesion as follows: disappearance of membrane system of chloroplasts, there were virus particles in some chloroplasts, and some sine or assembled virions in the mitochodria or cytoplasma but not in nuclei ,and intact subcellular structure in the cell far away from the infected center; In the rice leaves cell infected by Zhejiang isolate, some cells were full of virions, but the distribution of cells didn't form the belt, other cytopathological characters were similar to the cells infected by Yunnan isolate.

RDV云南分离株浸染的水稻叶片中，充满病毒粒体的细胞呈条带状分布，呈现出明显的侵染和增殖中心，在充满病毒粒体的细胞周围，相邻细胞有不同程度的病变，叶绿体膜系统消失，部分叶绿体中含有病毒颖粒，线粒体残体及细胞质中有分散或聚集的病毒粒体，细胞核中未见病毒粒体，离侵染中心较远的细胞中亚细胞结构较完整；RDV浙江分离株侵染的水稻叶片中，部分细胞充满病毒粒体，无明显的条带状分布，其他细胞病理特征与RDV云南分离株侵染的水稻叶片相似。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。