分离

In the process of evaporation, under the direction of phase diagram method, the main purpose is how to separate kinds of salt by-product, enrich Lithium, decrease the ratio of Mg/Li. The study shows that the Lithium can be concentrated to about 0.8%, the ratio of Mg/Li can be lowed to 10; In the process of precipitating, in order to separate the left Magnesium and Lithium, a lot of experiments were carried out with the Sodium carbonate or Calcium hydroxide and Sodium Sulfate anhydrate as precipitator of Magnesium, where the separation results were compared, and an optimal separation idea was proposed.

在蒸发阶段中，以相图分析为理论指导，着重研究了如何利用自然能源蒸发卤水，提取有用的镁盐和钾盐，并达到富集锂盐的目的，在此研究中，锂的浓度可富集到0.8％左右，镁锂比可降到10以下；在沉淀阶段中，分别研究了用纯碱、石灰乳和芒硝作为沉淀剂来分离镁锂，比较了分离效果、提出了最佳分离方案。

The separation of tocopherols and tocotrienols by using the method of adsorption chromatography on silica and on silica impregnated with silver nitrate have been discussed. Satisfactory separation could be obtained with argentation column chromatography by eluting batchwise with n-hexane containing increasing amounts of diethyl ether. More than 90% of vitamin E amount in eluate was tocotrienols.

以乙醚正己烷混合溶液为淋洗液，探讨了硅胶柱层析和硝酸银硅胶柱层析分离生育三烯酚和生育酚的效果，结果表明：通过改变淋洗液中乙醚含量可以将生育酚和生育三烯酚大部分分离：硝酸银硅胶柱层析分离生育三烯酚和生育酚的效果明显高于硅胶柱层析，其洗脱液中生育三烯酚可占V〓总量的90％以上，最高达98.47％。

Armillaria luteo-virens is a ectomycorrhizal fungus symbiotic with some species of Kobresia in Tibet Plateau, China. A strain was recently isolated from fruitbody tissue of the fungus. To determine whether the strain is the pure culture of the fungus, the internal transcribed spacer region and the first intergenic spacer region (IGS-1) of the nuclear ribosomal DNA of fruiting body and the isolated strain were sequenced and compared. Further, the 5.8S/ITS and IGS-1 sequences of the species were BLAST matched with GenBank database on homology and a phylogenetic tree was constructed based on 5.8S/ITS sequence and two phylogenetic trees were constructed separately based on two IGS-1 sequences.

首次从采自青藏高原、与高原牧草嵩草属Kobresia草本植物形成外生菌根的黄绿蜜环菌Armillaria luteo-virens子实体中分离获得一组织分离菌株，运用rDNA-ITS和rDNA-IGS-1测序技术对该组织分离菌株是否为黄绿蜜环菌的纯培养菌种进行分子鉴定，并基于黄绿蜜环菌的5.8S/ITS和IGS-1序列进行核酸序列数据库GenBank同源性检索比对、构建系统发育树。

Based on morphological characteristics and sequence alignment analysis of rDNA ITS region, 16 strains isolated from diseased plants of Setaria viridis from Beijing, Hebei and Henan, two strains isolated from diseased plants of Chloris virgata and Eleusine indica from Henan, and one strain isolated from diseased plant of Avena fatua from Qinghai, were identified as Bipolaris setariae.

经形态学鉴定和rDNA ITS序列分析，16株分离自北京、河北、河南发病狗尾草的菌株、2株分别分离自河南发病虎尾草、牛筋草的菌株和1株分离自青海发病野燕麦的菌株被鉴定为狗尾草平脐蠕孢Bipolaris setariae。

The full F gene was sequenced and compared with the published sequences of virulent and avirulent strains of NDV in GenBank database.

根据分离株序列与GenBank中NDV相关毒株序列比较分析，结果表明，所分离的6个分离株归属为3个NDV基因型，其中有基因Ⅶd亚型（3株）、基因Ⅵ型（2株）和基因Ⅲ型（1株）。

Among them, strains of Bacillaceae occupied the predominance with 59.1% in various wheat organs. Oxytetracycline resistant strains of Bacillus licheniformis were isolated from wheat rhizosphere, root and stem indicating their high abilities of spreading. Opportunistic human pathogenic bacterial strains with oxytetracycline resistance such as Bacillus cereus and Stenotrophomonas sp. were also isolated from various wheat organs. No oxytetracycline resistant bacterium could be detected from wheat seeds.

土霉素抗性的Bacillus licheniformis在小麦根际土壤、根、茎等器官中都分离得到表明该抗性菌种在小麦内生系统中具有较高的传播性；具有土霉素抗性的人类机会致病菌Bacillus cereus和Stenotrophomonas属的细菌在小麦的根际土壤、根以及叶子中也均分离到，但是在小麦种子中没有分离到任何土霉素抗性的内生菌。

Isolations of the s.aureus insoluble peptidoglycan:(1) the s.aureus insoluble peptidoglycan was gained by cold, hot and hypersound affection repetat of s.aureus(ATCC25923);(2) the s.aureus insoluble peptidoglycan was gained by 10% trichloracetic acid, 5% trichloracetic acid,NH4HCO3 buffer with trypsin digestion of s.aureus(ATCC25923) in order. 2. Isolations of the s.aureus soluble peptidoglycan and quality control:(1) the s.aureus soluble peptidoglycan was released by short dose benzylpenicillin sodium and purified by sephadex G-100.(2) The quality control in the process of isolation: the soluble peptidoglycan was detected by SLP reagent kit; the LPS was detected by kinetic turbidimetric limulus test;and the protein was detected by improved LOWRY reagent kit.

金黄色葡萄球菌可溶性肽聚糖的分离提取及其质量控制(1)采用青霉素钠诱导法和葡聚糖G-100(Sephadex G-100)凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖；(2)金黄色葡萄球菌可溶性肽聚糖提取过程中的质量控制：采用SLP试剂盒对提取物定性检测，应用动态浊度法鲎试验测定提取物中内毒素含量，采用改良LOWRY试剂检测提取物中蛋白的含量；(3)采用青霉素诱导和Sephadex G-100凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖的条件：青霉素使用量、加入青霉素后孵育时间和菌液浓度等优化选择。

Taking the integrated bioreaction and separation process as guide, a new technique, viz. the technique of in situ product removal based on the adsorption of the product by macroreticular resin JD-1 directly from the fermentation broth, was developed for pristinamycins production.

以生物反应与分离耦合的集成化思路为指导，建立了以JD-1大孔吸附树脂直接从发酵液中吸附分离目标产物的原位分离技术并耦合普那霉素发酵的新方法。

Therefore, the ways to their separation and identification are different, except similar separation method for caproic acid bacteria and butyric acid bacteria and similar property identification method for acetic acid bacteria and lactobacillus, the other methods are quite different.

摘 要：着重研究粮食酒发酵中4类产酸菌的分离，其中己酸菌与丁酸菌同属梭状芽孢杆菌，为厌氧菌，醋酸菌为好氧菌，乳酸菌则微好氧，因此各自有不同的分离鉴定方法，其中己酸菌与丁酸菌分离方法相似，丁酸菌与乳酸菌的性能鉴定方法相似，其他则各有特色。

The primary studies on insecticidical and fungicidical components of endophytic fungus in Celastrus angulatus have been carried out from September, 2000 to June, 2002.The results are as follows: Taking PDA and Czapek as separating culture medium, 39 strains of endophytic fungus were separated from the bark, phloem and xylem of the root and twig of Celastrus angulatus.

作者于2000年9月至2002年6月对杀虫植物苦皮藤中内生真菌的杀虫杀菌活性物质进行了初步的研究，取得了以下结果：采用常规的内生真菌分离方法，以马铃薯葡萄糖培养基、Czapek培养基为分离用培养基，从苦皮藤根和侧枝的表皮、韧皮部和木质部中分离得到39株内生真菌。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。