分离

We investigated constituent of triterpenoid saponins ofAlbizzia, two new compounds together with two known compounds were isolated from Albizzia julibrissin Durazz. by using column chromatography (macroreticular resin, silica gel, Sephadex gel, reverse phase silica gel),preparative HPLC methods et al.On the basis of spectroscopic analysis, including IR,ESI-MS,~1H-NMR,~(13)C-NMR,HMBC,HMQC,~1H-~1HCOSY and chemical methods, the structure of two new compounds were identified as 3 - O -[β-D-xylopyranosyl(1→2)-β-D-fucopyranosyl (1→6)-β- D -2- deoxy - 2 - acetoamidoglucopyranosyl] -21-O-[(6S)-2- trans- 2,6-dimethyl - 6 - O-β- D - quinovopyranosyl -2,7- octadienoyl] - acacic acid- 28 - O-β-D-glucopyranosyl(1→3)[α-L-arabinofuranosyl(1→4)]-α-L-rhamnopyranosyl(1→2)-β-D-glucopyranoside acacic acid 3- O -β- D- glucopyranosy(1→3)-β- D- fucopyranosl(1→6) [β-D- xylopyranosyl (1→2)]-β-D-glucopyranoside ;two known compounds were acacic acid lactone 3- O -β-D- xylopyranosyl-(1→2)-β-D-fucopyranosl (1→6)- 2-deoxy -2 -acetoamido -β-D- glucopyranoside ; acacic acid lactone 3- O-β-D-xylopyranosyl(1→2)-α-L- arabinopyranosl (1→6)- 2- deoxy - 2- acetoamido -β-D-glucopyranoside . The study lays chemical foundation and chemical reference substance for enhancing quality standard of Albizzia julibrissin Durazz.

本研究论文在综述国内外对合欢属Albizzia三萜皂苷化学成分和药理作用研究进展的基础上，利用传统植化分离手段和现代分离技术，包括大孔树脂、硅胶、葡聚糖凝胶、反相硅胶等柱色谱，制备高效液相色谱法等技术从中药合欢皮中分离得到了4个化合物，其中，2个新化合物和2个己知化合物，并进一步通过现代分析技术IR，ESI-MS，~1H-NMR，~(13C-NMR，HMBC，HMQC，~1H-~1HCOSY等和化学方法鉴定了2个新化合物的结构分别是：3-O-[β-D-吡喃木糖基(1→2)-β-D-吡喃夫糖基(1→6)-β-D-2-去氧-2-乙酰氨基吡喃葡萄糖基]-21-O-[(6S)-2-反式-2，6-二甲基-6-O-β-D-吡喃鸡纳糖基-2，7-辛二烯酸基]-金合欢酸-28-O-α-L-呋喃阿拉伯糖基(1→4)[β-D-吡喃葡萄糖基(1→3)]-α-L-吡喃鼠李糖基(1→2)-β-D-吡喃葡萄糖苷，金合欢酸3-O-β-D-吡喃葡萄糖基(1→3)-β-D-吡喃夫糖基(1→6)[β-D-吡喃木糖基(1→2)]-β-D-吡喃葡萄糖苷；2个已知化合物结构分别是：金和欢酸内酯3-O-β-D-吡喃木糖基(1→2)-β-D-吡喃夫糖基(1→6)-β-D-2-去氧-2-乙酰氨基吡喃葡萄糖苷，金和欢酸内酯3-O-β-D-吡喃木糖基(1→2)-α-L-吡喃阿拉伯糖基(1→6)-β-D-2-去氧-2-乙酰氨基吡喃葡萄糖苷。

Methods: Density gradient centrifugalization and absorption technique wererespectively applied to detach peripheral blood mononuclear cells andperipheral blood lymphocytes,T lymphocytes were got by Nylon postabsorption method, and CD4+T and CD8+T lymphocytes divided with anti- CD4 andanti-CD8 antibody by alexin cytotoxic method.

密度梯度离心法分离外周血单个核细胞，玻璃器皿吸附法分离外周血淋巴细胞，尼龙棉柱法分离 T 淋巴细胞，补体介导的细胞毒法分别制备 CD4+T和 CD8+T 淋巴细胞，采用 T 淋巴细胞与角质形成细胞共培养技术研究银屑病患者外周血 T 细胞对角质形成细胞的影响，免疫组化法检测共培养角质形成细胞 Ki67、c-Myc 及 Bcl-xL 蛋白的表达。

Summed up the research progress that exceeds critical and extractive technology to apply at alkaloid to depart, analysed each elements of effect of influence alkaloid depart, outside waiting for common element except temperature, pressure, dissolvent, extractive time, alkalify reagent can improve the detached result of alkaloid, carry secretly agent rose to exceed the capacity of critical and extractive fluid.

综述了超临界萃取技术应用于生物碱分离的探究进展，分析了影响生物碱分离效果的各个因素，除温度、压力、溶剂、萃取时间等常见因素外，碱化试剂能够改善生物碱的分离效果，夹带剂则提高了超临界萃取流体的能力。

Sequence analysis revealed that nucleotides sequence of 28 kD protein gene varied among different isolates of wheat yellow mosaic virus.28 kD protein genes of the Luotian and Huangchuan isolates comprised 762 nucleotidesand were less 3 nt than others,located at the site of 326,327 and 336 nt,respectively.The different isolates shared homologies ranging from 92.1% to 96.9% in nucleotide sequence and 89.8% to 97.3% in amino acid sequence.

序列分析结果表明，小麦黄花叶病毒不同分离物的28kD蛋白基因的核苷酸序列存在一定的差异：湖北罗田和河南潢川分离物在第326，327和336位较四川雅安、江苏扬州及日本分离物缺少3个核苷酸，前两者核苷酸长度为762nt，后三者为765nt；不同分离物核苷酸序列同源性从92 1%到96 9%，相应的氨基酸序列同源性从89 8%到97 3%。

This paper presents the present development of rape combine home and abroad and the running principle of typical threshing and separating system. And comparative experimental investigations are done between different threshing and separating systems, such as rasp-bar-plate-tooth drum with grid concave, axial-flow -short-rasp-bar drum with grid concave and spike-tooth drum with grid concave.

本文详细介绍了国内、外油菜联合收割机研究现状及典型的物料脱粒分离系统的工作原理，对油菜联合收割机不同的脱粒分离部件组合的脱粒分离系统——纹杆—板齿-栅格凹板、短纹杆—栅格凹板、钉齿—栅格凹板脱粒分离系统进行了对比试验与研究。

Separate under the SMAS to 2.0 cm above the supraorbital edge and 1.5 cm above zygomatic arch, where the periosteum and superficial layer of deep temporal fascia were incised to expose fracture;②Separate under the SMAS to the supraorbital edge and zygomatic arch, and then as in method Ⅰ, incise at 2.0 cm above the supraorbital edge and 1.5 cm above zygomatic arch to expose fracture.③ As in method 2, separate under the SMAS to the supraorbital edge and zygomatic arch, and then incise the periosteum directly to expose fracture.

对27例面中部骨折行头皮冠状切口患者分别采用，方法Ⅰ：沿SMAS下分离至眶上缘上2cm处和颧弓上1.5cm处，先在此切开骨膜和颧深筋膜浅层，分离暴露骨折；方法Ⅱ：沿SMAS下分离至眶上缘和颧弓处，然后再在眶上缘上2cm和颧弓上1.5cm切开直至暴露骨折；方法Ⅲ：同方法Ⅱ分离至眶上缘及颧弓处并直接切开骨膜，暴露骨折。

The influence determinants such as the concentration of polymyxin E sulfate,heterogeneous proteins which contained in the fermentation broth,inorganic ions,pH value and temperature,all were closely related to surface tension and foaming properties were studied.

将泡沫分离技术有效应用生物分离过程，除分离设备结构和操作工艺外，被分离的生物表面活性物质在体系中的性能，特别是表面张力和泡沫稳定性能研究非常重要。

Unscrew the locking nuts on the release lever, meanwhile unscrew the adjusting nuts using a spanner, while doing that measure the gaps between the release levers and bearings by means of feeler gauge, until the gap reads 0.3-0.5mm. Repeat it for three release levers, their gaps should be the same, after that screw up the locking nuts, and check it to make sure.

b、旋松分离杠杆上的锁紧螺母，一面用扳手转动调节螺母，一面用厚薄规测量分离杠杆与分离轴承之间的间隙，直到间隙为0.3-0.5毫米为止，三只分离杠杆依次都调整好，再将锁紧螺母锁紧，然后复查一遍。

B. Unscrew the locking nuts on the release lever, meanwhile unscrew the adjusting nuts using a spanner, while doing that measure the gaps between the release levers and bearings by means of feeler gauge, until the gap reads 0.3-0.5mm.

b、旋松分离杠杆上的锁紧螺母，一面用扳手转动调节螺母，一面用厚薄规测量分离杠杆与分离轴承之间的间隙，直到间隙为0.3-0.5毫米为止，三只分离杠杆依次都调整好，再将锁紧螺母锁紧，然后复查一遍

In order to resolve the problem met in the pretreatment of traditional methods for Optical stimulated luminescence dating sample which can not obtain enough purity and quantity of quartz, a new quartz separation process in obtained, with contrast experiments, combined with the welldeveloped quartz purification techniques in glass production, finally a new quartz separation process is obtained.

为了解决光释光前处理中遇到的传统方法无法获得满足纯度石英的问题，通过大量对比实验，结合玻璃工艺中石英分离研究成果，最后获得了新的石英提纯流程，与传统方法相比，新方法通过化学分离避免了密度变化对石英长石分离的影响，从而解决了斜长石和岩屑在传统的重液分离中无法剔除的问题，新方法能够获得满足纯度要求和量要求的石英，同时，新方法比传统方法实验室工作效率更高。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。