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Pathogens were isolated from diseased leaves of Atractylodes in Xiaochang county of Hubei province. Firstly, we pathogenicity test was conducted based on Kochs rule. Secondly, we described the morphological characteristics of the conidia, conidiophore and beak of the pathogens growing on diseased leaf land PCA.

从湖北省孝昌县苍术种植基地的发病植株叶片上分离得到菌株,根据柯赫氏法则对分离自发病叶片的菌株进行致病性测定,然后描述了寄主发病叶片以及PCA培养基上分生孢子、分生孢子梗及喙的形态,测量其大小,观察分生孢子链的形状。

The results showed that conidia were thin -walled and smooth to thick walled and verrucose; fat bodies accumulated gradually in both conidium and conidiogenous cell, and finally laid over the inner wall of conidium and disappeared in conidiogenous cell.

分生孢子发育的超微结构研究表明,分生孢子壁的发育是有个由薄而光滑到厚而有疣的过程;期间脂肪体在分生孢子和产孢细胞中不断累积,最后脂肪体沿着内壁排列成一层。

The diseased leaf rate and the disease index were related closely to temperature, humidity and illumination etc. The study also showed that the conidium germinating of Pestalotiopsis versicolor started after inoculated for four hours and germinated well at pH5 to pH7. In addition, there was great appetency between the pathogen and the tea trees because the conidia germinated well on tea leaves rather than other habitats obviously.

在茶树叶面上,病原菌分生孢子萌发明显比非叶面条件下好,病原菌菌丝生长较快,说明此病原菌与茶树叶片有高度的亲和力和较强的适应茶树叶面微环境的能力;茶树轮斑病病原菌分生孢子在pH5~7范围内萌发及芽管伸长较好,pH过小或过大均不利于病原菌的分生孢子萌发和菌丝生长。

Fruit dry, of two mericarps united by their faces, and usually attached to a central axis, from which the mericarps separate at maturity; mericarps are variously flattened dorsally, laterally or terete; each mericarp has 5 primary ribs, one down the back, two on the edges near the commissure, and two between the dorsal and lateral ribs, occasionally with four secondary ribs alternating with the primary, the ribs filiform to broadly winged, thin or corky; vittae usually present in the furrow (intervals between the ribs sometimes called the valleculae) and on the commissure face, rarely also in the pericarp, sometimes obscure.

果干燥,二分果瓣,通常附着在果瓣柄上,成熟时分离,分果瓣背面不同程度扁平,横向或者圆柱状;每分果瓣有5条主棱,一条背棱,2条侧棱,3条中棱,有时有4条与主棱互生的次级棱,棱丝形到形成宽翅,薄或木栓质沟槽和分果的合生面通常有油管(在棱和棱之间有沟槽,很少果皮上有油管,有时不明显。

Order of imperfect fungi lacking conidiophores of having conidiophores that are superficial and not enclosed in a pycnidium.

半知菌的一个目,缺少分生子柄或有分生子柄,生在表面,但不包含在分生孢子器内。

In order to understand the biology of apple ring spot fungi further and solve the problem of less conidiophores generated during their inducing culture in vitro, it was studied that the effect of culture medium pH, incubation temperature, light sources, and light regiment on growth of mycelia and generation of conidiophores, and the removing of mycelia on generation of conidiophores.

为进一步了解苹果轮纹病菌的生物学特性,解决其在离体培养条件下产生分生孢子很少的问题,研究了培养基pH值、培养温度、光源和光照条件对菌丝体生长和分生孢子发生的影响以及刮除菌丝体对分生孢子发生的影响。

Immunogold labeling demonstrated that there was no chitin and cellulose in young and thin conidial walls; chitin appeared in the well-developed wall in later stage of conidial development and little cellulose, however, was detectable in the wall of mature conidia.

免疫金标记结果显示,幼嫩的分生孢子壁中缺乏几丁质和纤维素,只有在成熟的分生孢子壁中含有几丁质;出乎意料的是在成熟分生孢子中发现有少量纤维素的存在。

On PDA plate, colonies grow rapidly to 12.5~37.2 mm/21d, phialide discrete, arising solitary from the aerial mycelium, septated at the base or middle, hyaline, smooth, 14.9~23.2μm long, 1.9~3.1μm at base, tapering to less than 1.0 μm at tip; conidia hyaline, reniform to elliptical, bulk is (4.6~5.9)μm×(2.1~3.4)/lm. These morphological characteristics are significantly different from the well-described Neotyphodiurn species. Their phylogenetic relationship remains further researches.

ciliaris12株Neotyphodium属真菌为材料,详细研究了我国干旱地区Neotyphodium属真菌的形态学特征:在PDA平板上生长很快,21 d生长12.5~37.2 mm;分生孢子梗长14.9~23.2μm,基部宽1.9~3.1μm,顶端小于1μm,通常基部或近基部有隔膜;分生孢子无色透明,卵圆形或肾形,单个顶生,(4.6~5.9)μm×(2.1~3.4)μm,与其它已知Neotyphodium属真菌形态差异显著,需要进行更深入的研究。

To obligatory pathogens like Pseudoperonospron cubensis, it could not only hold back the infection of hypha,but also heal the spot where infected by hypha.(8)The study on mechanism of action showed that the crude extract of S-5210 had a strong inhibition of the mycelia growth and spore germination which led to some abnormal growth such as the twisted and inflated mycelia, the abnormal increased branching, the broken mycelia wall and leakage of cytoplasm from hyphal tips.Alao the spores and the rate of germination were decreased, the germ tube was malformed in shape et al.The pathogenicity of hypha and spore was weakened.

8S-5210 活性产物的拮抗机理初步研究表明:S-5210 粗提物能强烈抑制病原菌菌丝的生长和孢子的萌发,引起病原菌菌丝扭曲或膨大、分枝增多、分枝顶端细胞壁破裂、原生质外溢;分生孢子数减少,孢子萌发率降低,芽管畸形等异常现象;菌丝和分生孢子致病性减弱。

Biological characteristics of the pathogen and the resistance of 13 cultivars of tea plant to tea brown blight were studied by means of natural infection in the field and artificial inoculation in vitro. Resistant cultivars and susceptible cultivars of tea plant were divided. And the resistance mechanism was researched tentatively. The findings are as follows: 1 Some biological characteristics of the pathogenic fungus The experiment result showed: the optimum temperature for the growth of fungus mycelium was from 24 to 27℃, the optimum temperature for sporulation ranged 22 to 30℃. Treated at 54℃ in 10 minutes, the spores didn't germinate. The optimum pH for the growth of the mycelium was 5.1~5.9, the optimum pH for sporulation ranged 5.0~6.0. Light had no much effect on the growth of fungus mycelium, but had much effect on the reproduction of the spore.

首先,研究了茶云纹叶枯病病菌的生物学特性;其次,通过田间抗性调查和室内人工接种鉴定,对 13 个茶树品种进行了抗病性鉴定;第三,在前人研究的基础上,对茶树抗病机理作了更深入的研究,结果发现: 1 病原菌的生物学特性病原菌的分生孢子萌发的最适温度范围为 24~27℃,菌丝生长的最适温度范围为 22~30℃;致死温度为 55℃;菌丝生长的最适 pH 范围为 5.1~5.9,孢子萌发的最适 pH 范围是 5.0~6.0;光照对病菌生长速度及分生孢子萌发影响不大,但对其产孢的影响很大,特别是有光照与无光照处理之间差异显著,光照有利于分生孢子的形成。

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