分生孢子的

In the stage of early hypha growth, spore germination, rapid filamentous growth stage and appressorium formation, the STK1 gene expression could be detected.

在产生分生孢子前、产生分生孢子时、菌丝的对数生长期和产生附着胞时都可以检测到STK1基因的表达。

Isolates isolated from maize and 3 isolates from rice, wheat, adzuki bean in ten provinces were identified as Curvularia lunata. Based on the culture characters, such as color of colony, aerial myeelia, the number of conidia, there were some difference among 43 isolates. Meanwhile, five types of conidium were compared in culture characters and virulence. In each single conidia culture there were different morphic conidia also and no difference in virulence was found among five single-conidium cultures.

收集并分离我国10个省的玉米叶斑病病原物40个以及水稻、小麦、红小豆上的C.lunata分离物3个；对分离物进行培养性状、产孢能力及形态特征的研究结果表明：分离物在菌落颜色、气生菌丝的繁茂程度、产孢量的多少等方面存在明显的差异；同一分离物内分生孢子在形态方面有差异，不同形态单孢继代培养后仍出现多种形态分生孢子，但各单孢培养系在致病力上无差异。

The alternate irradiation of ultraviolet lamp and daylight lamp is favorable for production of pycnidium, and mature pycnidium couldn't form in dark condition.

紫外灯与日光灯交替照射有利于分生孢子器的形成，黑暗条件下不能形成成熟的分生孢子器。

Microscopic examination showed the conidiophores were fasciculate and brown, and that the basal cell was enlarged and hemispheric. Well-developed conidia were long-obclavate, obtuse at both ends, with five to eight transverse septa, which indicated that the pathogen was Bipolaris setaria.

形态观察结果表明，该病原菌分生孢子梗丛生，褐色，基细胞膨大呈半球形，成熟的分生孢子长椭圆形，两端钝圆，具5～8个隔膜，表明其为狗尾草离孺孢。

Biological characteristics of Curvularia lunataBoedijn had been preliminarily studied. Optimal mediums for pathogen growth were PSA,PDA and OMA.For mycelia growth,sporulation and conidial germination of pathogen,optimal temperature was 25～30℃,and pH 5～9.Among the tested carbon and nitrogen resources,sucrose,glucose,carbamide,sodium nitrate,etc were beneficial for mycelia growth and sporulation.The conidia germination is strict in RH requirement:Conidia can germinate at RH 81%～98%,or have 100% germin...

香蕉大灰斑病菌适合生长的培养基有PSA、PDA和OMA；菌落生长、产孢和孢子萌发最适宜的温度为25～30℃，pH 5～9；在碳源和氮源中，蔗糖、葡萄糖、尿素、磷酸氢二氨和硝酸钠有利于病菌生长和产孢；分生孢子萌发对湿度有严格的要求，RH≥80%以上分生孢子萌发，随湿度增加萌发率增高；光照对菌丝体无显著影响，光暗交替有利于孢子的形成；孢子致死温度为55℃(10min)。

On PDA plate, colonies grow rapidly to 12.5~37.2 mm/21d, phialide discrete, arising solitary from the aerial mycelium, septated at the base or middle, hyaline, smooth, 14.9~23.2μm long, 1.9~3.1μm at base, tapering to less than 1.0 μm at tip; conidia hyaline, reniform to elliptical, bulk is (4.6~5.9)μm×(2.1~3.4)/lm. These morphological characteristics are significantly different from the well-described Neotyphodiurn species. Their phylogenetic relationship remains further researches.

ciliaris12株Neotyphodium属真菌为材料，详细研究了我国干旱地区Neotyphodium属真菌的形态学特征：在PDA平板上生长很快，21 d生长12.5~37.2 mm；分生孢子梗长14.9~23.2μm，基部宽1.9~3.1μm，顶端小于1μm，通常基部或近基部有隔膜；分生孢子无色透明，卵圆形或肾形，单个顶生，(4.6~5.9)μm×(2.1~3.4)μm，与其它已知Neotyphodium属真菌形态差异显著，需要进行更深入的研究。

Commassie brilliant blue staining can clearly display the different structures of Sphaerotheca fuliginea, such as germinating conidia, initial germtubes, hyphae and conidiophores, and hardly stain the host tissue, presenting a striking contrast between fungus and host tissue.

考马斯亮蓝组织透明染色方法可以清楚观察到白粉病菌的5个发育阶段，即萌发的分生孢子、初生芽管、胞间菌丝、分生孢子梗和后期菌落。考马斯亮蓝几乎使寄主组织不着色，不产生背景色干扰，而菌体变深蓝色。

The conidial germination need some special nutrients. The best carbohydrates and nitrogen for conidial germination are 1% saccharose and 1% glycin; the proper temperature was 20-30℃ and the optimal temperature was 25℃; the optimal pH was 5-6; the light had no obvious promotion effect on spore germination. The spores could not germinate above 52℃ for 10 min.

分生孢子萌发适宜碳源为1%的蔗糖溶液，适宜氮源为1%的甘氨酸溶液；孢子萌发适宜温度为20~30℃，最适温度25℃；最适pH为5~6，光照对孢子萌发没有明显的促进作用，分生孢子致死温度及时间为52℃10 min。

After the microscope through the cultivation of detection, slender hyphae, bifurcation, and short stem hyphae collateral for its health or the alternate on the branch, the branch can continue to branch, forming two, three branch , is the end of vase-shaped branching stems, spores observed liquid spherical sporangium producers body, the formation of conidia oval eggs, smooth-walled.

通过培养后显微镜观察检测，菌丝细长、分叉多，梗为菌丝的短侧枝，其上对生或互生分枝，分枝上又可继续分枝，形成2级，3级分枝，分枝末端即为瓶状梗，孢子液制片观察到球形孢子囊体，分生孢子卵形成椭圆形，壁光滑。

After pathogenic fungi identified,we found 26 of them can cause cotton leaf spot. The 26 pathogenetic strains were carried out morphological and molecular identification of the firm. The initial identification of the pathogens is through the morphological observation such as the determination of growth, colony morphology, conidia and conidial stalk.

通过对生长量的测定，菌落形态，分生孢子和分生孢子梗等形态学观察进行形态学鉴定；通过PCR扩增真菌核糖体基因转录间隔区序列并测序，并将序列在GenBank上进行同源性比对和构建系统系统发育树等方法进行分子鉴定，鉴定出这26株真菌分属链格孢属、镰孢属、黑孢属、刺盘孢属、匍柄霉属等5个属。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。