分激

In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

In the computation of aerodynamic forces, the present work is based on the work of Morino et al., but the following aspects are improved:(1) In computing the steady transonic aerodynamic load, the steady transonic nonlinear integral equation is solved by relaxation-iteration method in this thesis, instead of solving the time dependent transonic nonlinear integral equation, so that the computing time is saved greatly;(2) The influence coeifficients represented by volume integral are transformed to surface integral by using the Gaussian Theorem, so the analytical form of these coeifficients can be obtained and this leads to be more convenient to analyse and compile computer program;(3) The shock capturing method is used in every time step in present work, no shock moving term is added in the integral equation, so that it is more convenient and simpler to treat.

在气动力计算方面，本文基于Morino等人的工作，作了如下几方面的改进：（1）在计算定常跨音速流场（作为非定常绕流计算的初场）时，本文采用松驰迭代法直接求解跨音速定常非线性积分方程，而不是采用时间相关法求解非定常非线性积分方程，这样大大节省了计算机时；（2）将以体积分形式出现的影响系数化为面积分，并获得解析公式，这样便于分析和编写程序；（3）对运动的激波，本文通过在每一个时间步长上采用激波捕捉法而得到，而不是在积分方程中附加激波运动项，因而处理起来简单方便得多。

To judge two groups people in the day that people join in and 3 months later by brief psychiatric rating scale and nurse observe scale.results: comparing point value at the time of joining , 3 months later, the total score of nosie ,social ability, social interest and personal hygiene are apparently higher, irritableness, psychosis performance, retraction, depression and bprs are much lower.

结果：3个月后研究组患者的nosie量表的社会能力、社会兴趣、个人卫生3因子分明显高于入组时的分值，而激惹、精神病表现、退缩、抑郁4因子分和bprs分明显低于入组时的分值；与对照组比较：研究组患者在精神病表现、社会能力，社会兴趣、个人卫生、激惹、迟滞、抑郁等评分有显著性差异。

Summary This paper analyzes the working principle and stability of a transistor mul- tivibrator operating as a frequency divider,with both sides being synchronized by the input signal.It is found that the frequency division stability of this circuit can further be increased when the multivibrator operates in the second class of oscillating condition,and that the optimum operating point is best determined from the actual base voltage changing characteristic.

本文分析了晶体管自激多谐振荡器用作双侧同步分频电路的一般理论和分频稳定性，并考虑基极电压变化规律来确定最佳分频的工作状态和运用点，同时对同步脉冲的引入问题亦作了原理性补充，还提出了振荡于第二类状态下的自激多谐振荡器可获得高稳定性的分频，以及此类双侧同步分频电路的设计方法。

On the base of other researcher, the *** from the basic equations of stable current field to derived the expressions of boundary problems under 3-D condition. Without considering the EM effect and using the generalized variation principle, we can get the corresponding variation equation. Thronging subdivide the 3-D model, interpolation, analysis, and general synthesis with the triangular prism element, we can carry out the simulation on frequency-domain IP\'s parameters under level and undulate topography.

在前人工作的基础上，在忽略电磁效应的情况下，从稳定电流场的基本方程出发，推导了三维区域满足的边值题目，用广义变分原理得到相应的变分题目，通过用三棱柱单元对大地模型进行剖分、插值、总体集成，最后解方程，得到每个节点上的复电位值，最后实现了水平和起伏地形等条件下的激电参数的数值模拟。2。

The ground-state binding energy of an exciton confined in quantum wires and quantum wells in the presence of a uniform electric field has been calculated under the effective-mass approximation using variational approach.

本文采用变分法研究了外加电场下无限深方形量子线中重空穴激子的束缚能，我们用平面波展开的方法求解在电场作用下无限深方形量子线中电子和空穴的基态能和基态波函数及低激发态的能量和波函数，并用所得到的波函数进一步计算激子的基态及低激发态的束缚能，最后计算了激子的Stark能移。

Laser imaging imagesetter to laser are crystal moduators or galvanometric system of electronic color screen, the computer-controlled laser or blocked in order to reach a record of silver halide film forming images on exposure, high precision by the treatment of development for World silver halide film.

激平影像照排机机，以激平为平源的晶体调制器或振镜编制举行的激平电子分色增网，施计算机把持使激平堵住或被不准以到达纪录银盐软片上曝平变成影像，不纬显影得回矮精度的网点银盐底片。

Comparative study of the properties of both ground and excited states of metal-free indoline dyes for dyesensitized solar cells revealed: as the number of rhodanine rings increases, the energy difference between HOMO and LUMO decreases and there is a red shift in the absorption spectrum with the binding energy increased, and the transition dipole moment decreased; Based on an analysis of cha...

对比研究吲哚染料的基态和激发态性质，揭示了随着绕丹宁环数目的增加，HOMO和LUMO的能量差减少，吸收光谱红移，但是束缚能增加，跃迁偶极矩减少；依据电荷差分密度，电荷和能量从苯乙烯转移到吲哚环和绕丹宁；电子空穴相干性主要表现在吲哚环和绕丹宁环，对于吲哚染料系统，存在一个绕丹宁环的体系的激子数目为30个原子，带有两个绕丹宁环的体系激子数目有40个原子。

Holographic hot gold foil is thin with hot stamping feature laser treatment for holographic foil, forming a 2-d, 3-d, dot-matrix, spiral-shaped, holograms and other images with high-gloss paragliders and may attract hundreds of thousands of color mistify that overcomes the ordinary form of blocking technology, poor quality, lack of security bugs such as the effect.

激平残息烫金箔是差具有烫印功用的薄箔举行残息激平办理，形不败二维、三维、点阵、旋状、分不败残息等图像，具有矮平泽、奼紫嫣红并可幻化万千的色彩，降服了别致烫金技艺活命步不天驳杂、质度不矮、充足不攻伪不败果等弊端。

Mathematical model of nonlinear vortex-induced vibration was presented for predicting the nonlinear dynamic responses of submerged floating tunnel tethers which were subjected to wave, current and oscillatory displacements at their upper end in horizontal and vertical directions.

建立了水中悬浮隧道锚索在波流场中涡激振动的非线性数学模型，考虑了锚索的几何非线性对其涡激振动的影响，并应用有限差分法求解了锚索的运动控制方程。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。