分泌作用的

GnRH could markedly suppress progesterone secretion of cultured GCs with or without FSH and LH co-treatments. E〓 could invert the suppression of GnRH completely, also had the additive action on secreting progesterone with GnRH co-treatment. Mel partially released the suppression action of GnRH.

GnRH能强烈地抑制体外培养的颗粒细胞分泌孕酮，其抑制作用几乎不受FSH和LH影响；Mel仅表现部分地缓解了GnRH的抑制效应，但没有逆转。E〓不仅可以完全逆转GnRH的抑制作用，进而与GnRH叠加促进颗粒细胞分泌孕酮。

GnRH could mar Red I y suppress progesterone secretion of cultured GCs with or without FSH and LH co-treatments. E2 could invert the suppression of GnRH completely, also had the additive action on secreting progesterone with GnRH co-treatment. Mel partial ly released the suppression action of GnRH.

GnRH能强烈地抑制体外培养的颗粒细胞分泌孕酮，其抑制作用几乎不受FSH和LH影响；Mel仅表现部分地缓解了GnRH的抑制效应，但没有逆转。E_2不仅可以完全逆转GnRH的抑制作用，进而与GnRH叠加促进颗粒细胞分泌孕酮。

DCs acquired by our reformed methods express both CD83 and CD 14 molecules highly, and have a higher density than other domestic reports. The higher TNF in DCs culture medium of HC patient suggests DCs in patient still have antigen presenting ability and by optimization the culture medium would improve its presenting ability and have a potential value in design and application individual vaccine. Although antigens pulsed DCs have a decrescent antigen presenting ability but BCG HSP70 could induce its mature and improve its presenting ability. Suggests BCG HSP70 would be a useful mature inducer. Lymphocytes primed by DCs based HC vaccine have the specific cytotoxicity against HCC lines. The CTL after freezing and anabiotic could prophylaxis and therapy HC xenograft on nude mouse. The results also suggests that CD4〓 lymphocytes play a important role in HC with a good differentiation and would be useful in treatment this kind of HC. After being activated by Peptide LLNQHACAV of hAFP and apoptotic HCCs pulsed DCs respectively, the culture medium of activated lymphocytes both contains a high level Th1 cytokines IL-12 and TNF. Primed lymphocytes appeared a characteristic of NK cells. DCs not only inhibited the growth of human HCC and other cancer cells in vitro but also prevented the growth of HC xenograft on nude mouse in vivo. There are at least three kinds of mechanism playing important role in DC based vaccine ,there are inhibition of DCs, HC specific CTL and cytokines pathway.

诱导出的DC共同表达CD83和CD14分子，CD83分子表达明显高于国内报道；肝癌患者DC培养上清中TNF水平高于健康人，提示肝癌患者DC仍具一定的抗原呈递能力，适当调控可使其行使APC功能，以期在肝癌个体化疫苗中发挥作用；DC负载肝癌可溶性抗原后，抗原呈递能力降低，BCG HSP70可促进DC成熟，增加其抗原呈递能力，预示BCG HSP70有可能成为促进DC活化和成熟的另一重要分子；肝癌DC疫苗在体外诱导肝癌特异性淋巴细胞，活化的淋巴细胞在体外对肝癌细胞的杀伤以特异性CTL为主，同时分泌较高水平Th1型细胞因子IL-12和TNF，并抑制4种肝癌细胞生长；冷冻复苏后的肝癌特异性淋巴细胞可以预防和抑制人肝癌裸鼠皮下移植瘤，提示DC负载肝癌可溶性抗原后诱发的MHC-Ⅱ类限制性CD4〓T细胞有可能在分化程度高的肝癌治疗中发挥作用；用DC和HLA-A2〓DC分别负载凋亡肝癌细胞和hAFP218-226位LLNQHACAV HLA-A2限制性九肽，在体外诱导肝癌特异性淋巴细胞，活化后的CTL细胞分泌较高水平的Th1型细胞因子IL-12和TNF，并具较强杀伤活性，此CTL同时具备NK细胞特征；DC对肿瘤细胞的抑制作用可能是通过吞噬实现的，Fas-L在DC抑制中也起一定作用；DC对人肝癌裸鼠皮下移植瘤的抑制率为97％；在肝癌DC疫苗的作用中，至少联合3种以上机制，即通过DC的直接作用、肝癌特异性CTL和细胞因子途径直接或间接地杀伤和抑制肝癌细胞。

LH may be involved in the functional regulation of fundic glands through autocrine or paracrine activity.

大鼠胃壁细胞和胃主细胞既能产生LH，又能表达LHR，说明LH对胃壁细胞和胃主细胞功能作用可能是通过旁分泌或自分泌的作用来实现的。

Both Fas and NO can lead chondrocyte apoptosis respectively and cause articular cartilage destruction. IGF-Ⅰ, TGF-β, bFGF, BMP and other growth factors are polypeptide agents that can influence cell activity by signal convection. They can accelerate chondrocyte proliferation and proteoglycan synthesis, play the local regulation action on formation and plerosis of bone and cartilage tissue by autocrine or paracrine. They have the ability to induce cartilage formation. Some investigations showed that growth factors can influence chondrocyte metabolism, synthesis of specific Ⅱ type collagen and proteoglycan by co-operation and inhibition. 1. 3 Situation of OA therapeutics The therapeutic methods of OA mainly comprised non-drug treatment, drug treatment, operation treatment, tissue and genetic engineering, et al. Drug treatment is the chief method among them.

若其活性发生改变，则将导致关节软骨基质成分的丢失和进行性破坏；软骨细胞凋亡与OA的发病密切相关，Fas与NO可各自独立介导软骨细胞凋亡，造成关节软骨破坏；IGF-Ⅰ、TGF-β、bFGF、BMP等生长因子是一组通过细胞间信号传递影响细胞活动的多肽因子，具有促进细胞生长、增殖与合成等作用，可通过自分泌或旁分泌方式对骨和软骨的形成和修复起局部调节作用，可促进软骨细胞增殖、分化与蛋白多糖的合成，具有较强的诱导软骨形成的能力，研究表明多种生长因子相互促进、相互抑制，以协同或拮抗方式影响软骨细胞代谢，影响软骨细胞特异性Ⅱ型胶原和蛋白多糖的合成分泌。

In fact, PTHrP is polyhormone and a family of peptides arises from the action of proteolytic enzyme.

PTHrP主要通过自分泌、旁分泌和胞内分泌的作用方式发挥作用。

Results:①The amount of human colon carcinoma cell line SW480 treated by quercetin decreased. The morphology of partial SW480 cells was shrunk volume, integrated cell membrane, condensed cytoplasm, pyknotic chromatin, nuclear fragmentation. Apoptotic Corpuscles were found by electron microscope.②MTT colorimetric assay showed quercetin inhibited the growth of human colon carcinoma cell line SW480 in a time- and dose-dependent manner when the concentration of quercetin was 30、60、90μmol/L.③Flow cytometry analysis showed the cell cycle of SW480 cell was restricted in G1/S. G0/G1 phase rate increased and S phase rate decreased with increasing concentration of quercetin and time lasting.④ Zymogram analysis assay showed the secretion of matrix metalloproteinases in human colon carcinoma cell line SW480 treated by quercetin decreased. With increasing concentration of quercetin, the secretion of MMP-2 and MMP-9 decreased.⑤Immunohistochemistry method demonstrated the position expression of Cathepsin-D in SW480 cell was suppressed by quercetin in a time- and dose-dependent manner.

研究结果：经槲皮素处理的人结肠癌SW480细胞数量减少，部分细胞体积缩小，细胞膜完整，胞浆浓缩，核染色质固缩，细胞核碎裂，形成凋亡小体；MTT法检测显示当作用浓度为30μmol/L~90μmol/L时，槲皮素对人结肠癌SW480细胞的生长有抑制作用，其抑制作用随着作用浓度的增加和作用时间的延长而增强；流式细胞学发现槲皮素主要作用于人结肠癌SW480细胞周期的G1/S期，大部分细胞被阻断于S期，随药物浓度的升高和作用时间的延长，G0/G1期细胞比例逐渐增加，S期细胞比例逐渐减少；酶谱分析法检测显示不同浓度的槲皮素能够抑制人结肠癌SW480细胞分泌MMP-2及MMP-9，随浓度的升高，MMP-2及MMP-9的分泌量减少；免疫组织化学法显示不同浓度的槲皮素处理人结肠癌SW480细胞后，Cathepsin-D的表达随药物浓度的升高和作用时间的延长而降低。

These data suggest that ACh may have a significant modulating effect on the proliferation of pituitary adenoma cells by means of paracrine or autocrine, and the effect is mediated by muscarinic receptor.

该结果提示， ACh可能以旁分泌或自分泌的方式作用于垂体前叶细胞，对垂体腺瘤细胞增殖分化有调控作用，而且是通过ACh M受体来实现的。

Compared with development stage at the fortith day, total exudation rates of amino acids and organic acids in common genotype at the fifith day decreased sharply, while it kept rather stationary in potassium-rich genotype; At three growth phrases, tyrosine, serine and y-amidobutyricacid were dominant amino acids in root exudates of those two genotypes, malic acid was dominant organic acids, and the proportision of malic acid to total exudation increased with the prolongation of growth phase prolonged.

在3个生长期内，酪氨酸、γ-氨基丁酸和丝氨酸均为两类基因型根系分泌物中的主要氨基酸种类，苹果酸则是主要的有机酸类型，其在氨基酸和有机酸分泌总量中所占相对比例均随生长期的延长而升高。3籽粒苋根系分泌物处理后的土壤速效钾含量均高于清水对照处理，富钾基因型在低钾胁迫时的根系分泌物对土壤钾的活化作用明显大于一般基因型。

Compared with development stage at the fortith day, total exudation rates of amino acids and organic acids in common genotype at the fifith day decreased sharply, while it kept rather stationary in potassium-rich genotype; At three growth phrases, tyrosine, serine and γ-amidobutyricacid were dominant amino acids in root exudates of those two genotypes, malic acid was dominant organic acids, and the proportision of malic acid to total exudation increased with the prolongation of growth phase prolonged.

在3个生长期内，酪氨酸、γ-氨基丁酸和丝氨酸均为两类基因型根系分泌物中的主要氨基酸种类，苹果酸则是主要的有机酸类型，其在氨基酸和有机酸分泌总量中所占相对比例均随生长期的延长而升高。3籽粒苋根系分泌物处理后的土壤速效钾含量均高于清水对照处理，富钾基因型在低钾胁迫时的根系分泌物对土壤钾的活化作用明显大于一般基因型。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。