分泌

The secreted protein can reach to periplasm by the Sec-dependent system, and to export from cellular matrix by a machine composed of 12~14 proteins.

分泌的蛋白会先藉由Sec-dependent系统到达膜间质，再由一群12~14个蛋白所组成的分泌机器将分泌蛋白运送出细胞外。

Both Fas and NO can lead chondrocyte apoptosis respectively and cause articular cartilage destruction. IGF-Ⅰ, TGF-β, bFGF, BMP and other growth factors are polypeptide agents that can influence cell activity by signal convection. They can accelerate chondrocyte proliferation and proteoglycan synthesis, play the local regulation action on formation and plerosis of bone and cartilage tissue by autocrine or paracrine. They have the ability to induce cartilage formation. Some investigations showed that growth factors can influence chondrocyte metabolism, synthesis of specific Ⅱ type collagen and proteoglycan by co-operation and inhibition. 1. 3 Situation of OA therapeutics The therapeutic methods of OA mainly comprised non-drug treatment, drug treatment, operation treatment, tissue and genetic engineering, et al. Drug treatment is the chief method among them.

若其活性发生改变，则将导致关节软骨基质成分的丢失和进行性破坏；软骨细胞凋亡与OA的发病密切相关，Fas与NO可各自独立介导软骨细胞凋亡，造成关节软骨破坏；IGF-Ⅰ、TGF-β、bFGF、BMP等生长因子是一组通过细胞间信号传递影响细胞活动的多肽因子，具有促进细胞生长、增殖与合成等作用，可通过自分泌或旁分泌方式对骨和软骨的形成和修复起局部调节作用，可促进软骨细胞增殖、分化与蛋白多糖的合成，具有较强的诱导软骨形成的能力，研究表明多种生长因子相互促进、相互抑制，以协同或拮抗方式影响软骨细胞代谢，影响软骨细胞特异性Ⅱ型胶原和蛋白多糖的合成分泌。

For further understanding the effect of the strain on K-bearing silicate mineral, an experiment with KO2 strain cultured in nitrogen-containing and nitrogen-free medium was conducted. By putting mineral powders into the medium and measuring the concentration of capsular polysaccharides, we concluded that the strain can accelerate the weathering rate of silicate minerals. The results showed that the polysaccharides secreted by the strain in the growth process could help the bacterial to adhere to the mineral surface effectively, and created the bacterium-mineral complex, which formed a mircro-enviorment avail the release of potassium. Another result was that there was higher level of Carbonic Anhydrase activity, which revealed that some exo-protein or enzyme produced by the bacterial had postive impact on the process of releasing potassium ion. We carried out the bacterial fermentation for larger scale production of the bacterial secretion, which was used to sperate, and identify the small molecules related to the mineral-bacterial interaction. After the analysis, it was found that the strain can produce many kinds of small molecules, such as 2-Hydroxybenzoic acid, 4-Hydroxyphenylacetic acid, methyl-4-hydroxybenzoate, etc.

分别将菌株接入含有不同矿粉的培养基中培养，检测其在有氮、无氮培养基中荚膜多糖含量的变化，并在以钾长石和黑云母为矿源的情况下，比较研究了菌株在有氮、无氮培养基中对矿物的风化能力，通过一系列的实验，证实胶质芽孢杆菌在生长过程中所分泌的粘稠胞外聚糖可帮助细菌有效黏附在矿物表面，形成细菌-矿物复合体，并改变及维持该复合体内部的微环境，有助于该菌的解钾作用，而且细菌分泌的胞外蛋白质在该菌对含钾硅酸盐矿物的解钾作用过程中发挥重要作用；将胶质芽孢杆菌接入以钾长石和黑云母为矿源的有氮、无氮培养基中培养，检测到该菌碳酸酐酶活性的变化，并进行批量发酵后小分子酸性分泌产物的提取、分离、纯化与鉴定等方面的研究，结果表明，该菌可产生2-羟基苯甲酸，4-羟基苯乙酸，4-羟基苯甲酸甲酯等小分子物质。

In normal pregnancy, trophocyte secrete varies hormones, besides hCG, it participate in synthesis of steroid hormones, hPL and so on. JAR cell posses the function of secretion such hormones.

三、正常妊娠时滋养细胞分泌多种激素，除了hCG以外尚参与了类固醇激素，hPL等多种激素的合成与分泌，JAR细胞也具有分泌这些激素的功能。

From this study, hCG is a autocrine hormone for trophocyte, as a kind of autocrine hormone, hCG regulate trophoblastic tumor strain JAR cellular 〓 and P secretion at least in vitro.

从本研究结果看，hCG对滋养细胞而言也是一种自分泌激素，作为一种自分泌激素，至少调节着滋养细胞肿瘤细胞株JAR细胞的〓、P的分泌。

Methods test 1 256 samples from suspect uu or ct sufferers and infertile patients during 2007.results the positive rate of uu of 149 samples of prostate liquid is 42.3%(63/149), and the positive rate of ct of 136 samples of prostate liquid s is 19.9%(27/136);the positive rate of uu of 361samples of the secretion of the urinous tract is 21.1%(76/361),and the positive rate of ct of 346 samples of the secretion of the urinous tract is 10.4%(36/346);the positive rate of uu of 141 samples of semen is 11.3%(16/141),and the positive rate of ct of 123 samples of semen is 7.3%(9/123).conclusion there are significant differences in positive rate of uu and ct of 3 types of samples p.005.the positive rate of specimens of postate liquid is higher than that of the secretion of the urinous tract ,the positive rate of semen test is the lowest..the results from the uu and ct test of different types of specimens are different obviously .the most suitable specimen of test is prostate liquid.

对2007年1 256例怀疑感染uu或ct的患者和不孕不育症患者标本检测。结果：149例前列腺标本中uu阳性率为42.3%(63/149)、136例前列腺标本ct阳性率为19.9%(27/136)；361例尿道分泌物uu阳性率为21.1%(76/361)、346例尿道分泌物ct阳性率为10.4%(36/346)；141例精液uu阳性率为11.3%(16/141)、123例精液ct阳性率为7.3%(9/123)。结论：三种标本的uu、ct阳性率相差显著，p.005。前列腺标本检测的阳性率明显高于尿道分泌物标本，精液检测的阳性率最低。不同类型的标本检测的uu、ct的结果有很大的差异，送检的标本最好是前列腺液。

Bidentis were studied by adopting indoor bioassay. The results showed that the volatile matter of F. bidentis had not allelopathy to four receptor plants. The eluviated matter inhibited seedling growth of cotton obviously, the inhibition ratio of root and stem were 6.29% and 10.42% respectively. The aqueous extract of litterfall and root exudates had different inhibition to four receptor plants, The synthesis effectof all treatments was significantly greater than eluviated matter treatment except for B3 of the aqueous extract of root exudates, such as the synthesis effect of all treatments (A1, A2, A3, B1, B2, B3 and eluviation) were 44.74, 29.37, 14.5, 31.54, 16.52, 3.55, 1.48 respectively.

结果显示：黄顶菊自然挥发物对受体植物不表现化感作用；黄顶菊淋溶物对棉花幼苗生长表现出明显的抑制作用，对棉花幼苗高度和根长的抑制率分别为6.29%和10.42%；黄顶菊枯落物浸提液和根系分泌物浸提液对4种受体植物均表现出不同程度的抑制作用，除了黄顶菊根系分泌物浸提液 B3处理外，其它处理的综合效应均显著大于淋溶物处理，如黄顶菊枯落物浸提液（ A1、 A2、 A3）、根系分泌物浸提液（ B1、 B2、 B3）以及淋溶物等各处理对受体植物的综合效应分别为44.74、29.37、14.5、31.54、16.52、3.55、1.48。

Human SgⅢ is expressed widely as showed in Northern blot and its cDNA hybridizes to 2.2 kb and 1.9 kb bands in many tissues, with two additional 4.5 kb and 3.3 kb bands in brain.

Western印迹和免疫细胞化学实验证实，SgⅢ蛋白同其他分泌粒蛋白家族成员一样，通过分泌途径被分泌到胞外。

All C.neoformans contain serine protease; Serine proteinase perturbs the integrity of BMEC of the blood-brain barrier, improves open the level of BBB; Secreted serine protease from pathogenic fungi were virulence.

发现新生隐球菌分泌具有活性的胞外蛋白水解酶并含有丝氨酸蛋白酶成分；新生隐球菌自身分泌的丝氨酸蛋白酶可以干扰构成血脑屏障主要成分内皮细胞的完整性使得血脑屏障开放程度提高；致病性真菌分泌的丝氨酸蛋白酶可能是一种毒性因子。

HCRT potentiates NMDAR-mediated somatic release from LC neuronsApplication of 100μM NMDA evoked a pronounced secretion.This response to NMDA was subsequently enhanced by treatment with 100 nM HCRT for 5 min.

HCRT增强LC神经元NMDA受体介导的胞体递质分泌给予100μM NMDA可以显著引起分泌信号，随后用100 nM HCRT处理5 min，再次给予100μM NMDA可引起更大的分泌信号(增加大约40%)。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐