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Methods Hybridoma cell line excreting monoclonal antibody against total aflatoxins was produced by using B cell hybridoma technique.

方法利用B细胞杂交瘤技术,建立分泌抗总黄曲霉毒素单克隆抗体杂交瘤细胞株。结果建立了1株稳定分泌抗总黄曲霉毒素抗体的杂交瘤细胞株,命名为2G2。

Results:Hybridoma cell lines excreting monoclonal antibodies were developed and monoclonal antibodies against FB1 were prepared.

结果:建立了稳定分泌抗FB1毒素单克隆抗体的杂交瘤细胞株,分泌的抗体为IgG1亚类,分子量150KD,腹水稀释度为1:2.0×108,亲和常数为6.72×109 L/mol。

Something, such as the frothy secretion of spittle bugs, that resembles expectorated saliva.

粘液分泌物:某些昆虫的泡沫状的分泌物,与吐出的唾液相似

Methods Clinical examination was performed for all patients with vulvae itching and vaginal discharge diagnosed of vaginal candidiasis.

对以外阴瘙痒及白带增多为主诉,拟下生殖道假丝酵母菌感染的女性患者进行临床检查,先取阴道分泌物进行假丝酵母菌检查,同时取宫颈管分泌物采用荧光定量PCR测定UU、CT。

They interact,induce cascade amplification effect by activating a series of cell signals,lead to inter-attraction between tumor cells and neural fibers,appear the perineural invasion phenomenon.

肿瘤周围存在通过自分泌和旁分泌方式而来的神经生长因子及受体,它们相互作用,通过激活一系列分子信号途径引起级联放大效应,导致肿瘤细胞和神经纤维相互吸引而产生神经侵袭现象。

Twenty-four crossbred early weaned piglets with an average initial weight of 6.10±0.35kg,weaned at 28±1 day of age,were divided into four groups to determine the effect of supplemental acidifiers on gastric acid secretion,digestive enzyme activities, and intesinal microflora.

添加0.25 %复合酸提高了胃蛋白酶(P.10)和胃酸分泌(P.10),显著提高了十二指肠内容物中胰蛋白酶和淀粉酶活性(P.05),添加1.5 %柠檬酸有提高上述两种酶活性的趋势(P.15 ,P.10),但对胃酸和胃蛋白酶分泌没有影响,显著降低了结肠大肠杆菌数量(P.05)。

In this study, we analyzed 31 cases of PLCIS (13 apocrine and 18 nonapocrine subtypes) and compared the clinical, pathologic, immunophenotypic, and genetic characteristics of these cases with those of 24 cases of CLCIS.

本研究中,我们分析了31例PLCIS(13例顶浆分泌型和18例非顶浆分泌型)的临床、病理、免疫表型和分子遗传学特征,并将其与24例CLCIS做比较。

In conclusion, epididymosomes are small membranous vesicles secreted in an apocrine manner in the intraluminal compartment of the epididymis and play a major role in the acquisition of new proteins by the maturing spermatozoa.

总之,epididymosomes是一种小的膜状小泡,以顶分泌分泌方式存在于附睾管腔中,在精子成熟过程中获取新蛋白发挥着主要作用。

Effect of acid soil on dry matter accumulation and Al and nutrient contents of barleys differing in acid soil toleranceThe experiment was conducted by using 10 barley genotypes with different acid soil tolerance at seedling stages, each 5 genotypes for acid tolerance and sensitivity, to determine the difference in biomass accumulation, Al and nutrient content in the roots and shoots among genotypes under the condition of acid soil stress.

低pH和两种铝处理下植株各器官ca、Mg含量,茎杆和叶片P、K含量,叶片Fe、MO、Zn含量以及茎杆Zn、B含量和根系及叶片Mn含量均降低,铝福复合处理时降幅更大。100林MAI处理,大麦幼苗整个试验期间根系分泌的苹果酸、柠檬酸和唬拍酸含量与对照相比均增加,且耐铝基因型增幅大于铝敏感基因型,表明铝诱导这些有机酸分泌量增加可能与品种耐铝性有关。

On the termination date, the cultured explants were all examined by Western blot, HE and transmission electron microscope. Our results showed that after 12-days in culture, the cultivation treated with AS-ODN reduced the synthesis of AMBN and had a deformed dental cusp with thinner enamel matrix. Ultrastructure analyses showed that there was hardly any cisternae of the rough endoplasmic reticulum in the ameloblasts at the tip of the cusp of AS-ODN treatedexplants. However, on average the enamel matrix was thinner compared with that in the control group. Furthermore, the collagen fibers in extracellular matrix were found disorganized. These findings seemed to provide a direct experimental evidence that tended to indicate that the arrested AMBN translation in cultured tooth germs might result in the delay of the tooth development.

经用Western蛋白印迹检测表明,所设计的反义核酸对AMBN InRNA具有良好的封闭效果并成功阻断了牙胚对AMBN的表达;在缺乏AMBN情况下,与对照组相比,实验组牙胚在体外可以继续生长发育至钟状晚期,出现成釉细胞和成牙本质细胞的分化,成釉细胞可以分化成为分泌期型成釉细胞,胞浆中缺少合成蛋白质所必需的粗面内质网和高尔基氏体,缺乏溶酶体,表明对蛋白合成和脚的能力降低;实验组牙胚有牙尖形成和基质分泌,但牙尖形态异常,基质形成减少,牙尖周围基质最厚处为O.6卜m,明显薄于对照组的5.spin,基质中胶原纤维粗细不等,排列稀疏, 3 第四军医大学硕士学位论文未见钙化现象,充分证明了AMBN在牙胚发育中参与釉质基质形成和矿化过程,影响胶原纤维和牙本质基质的合成,促进成釉细胞对蛋白质的合成和釉质基质蛋白降解。

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