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ARID (AT-rich interaction domain) protein is a transcription factor family in higher eukaryotes that regulates cell proliferation, development, and differentiation. Specificity of DNA binding ability in this family prefers AT-rich sequences, but some ARID family proteins are not sequence-specific DNA-binding proteins or they do not bind AT-rich sequences. We found two genes that encode ARID in Giardia lamblia genome database, garid1 and garid2. We analyzed the function of garid1 first. AU1-tagged gARID1 was found to localize to nuclei. During encystation, gARID1 mRNA level decreased emphatically, but protein level increased. We also found that gARID1 can bind AT-rich initiator of the cwp1 promoter by EMSA. Mutation analysis revealed gARID1 binding sequence was AGATC and AATAAAATA. We used ChIP to demonstrate that gARID1 can bind cwp1 gene promoter in vivo.

ARID(AT-rich interaction domain)蛋白质家族是真核生物的一种转因子,在许多同种的真核生物有它的同源基因,这个家族的蛋白质通常与调控细胞的生长、发育和分化的作用有关,而这个家族的蛋白质和DNA的结合能,各种ARID蛋白质的专一性尽相同,过大致上偏好於和AT-rich的序结合;我们已经在形鞭毛虫的基因组中找到个含有ARID 的基因,分别是garid1和garid2,我们首先对於garid1做分析;将AU1标记接到gARID1转染形鞭毛虫,用免疫萤光染色可发现gARID1存在於细胞核中。gARID1的讯息RNA在囊体化后会明显下,过其阳性染色和蛋白质表现有明显增加;EMSA实验中也发现gARID1会明显的与cwp1基因启动子之AT-rich initiator结合,经由突变序分析,也显示gARID1的结合序为AGATC和AATAAAATA,随后我们也用ChIP证明gARID1在细胞内也的确会和cwp1基因的启动子结合。

Combining ion exchange process and silicon hydrolyze process Silicon dioxide mother nuclide with a specific particle size was prepared from sodium silicate through the dropwise technique , then the large particle size , high concentration and stable silica sol was prepared . The stability of silica sol also can be improved by enwrap .

和"单质硅一步溶解法"相结合的方法,用离子交换法制备晶种,并采用滴加工艺制备一定粒径大小的二氧化硅作为母核,在催化剂和分散剂共同作用下水解单质硅的方法使二氧化硅母核颗粒进一步增长成大粒径的二氧化硅颗粒,并另外还采用水溶性高分子对纳米SiO_2粒子进行"原位表面包覆"提高其稳定性,制备了大粒径,高浓度,稳定的纳米硅溶胶。

Now we have known that SARS-CoV encoded 11 varietal proteins. They have 4 constitutive proteins: spike protein, membrane protein, envelope protein and nucleocapsid protein. Spike protein trimer form spike and exsertion? virus peplos on the surface. Spike protein is archi-surface antigen component of virus, it has acceptor binding and membrane fusion activity. Spike protein has important effect at tissue tropism, cell-fusion and toxicity. Nucleocapsid protein can bind genome RNA to shape neucleocapsid. Membrane protein and envelope protein are necessary constituent to shape virus peplos. Spike protein is I-type membrane glycoprotein, it mediate virus binding with host cell epimembranal acceptor and to fuse host cell membrane.

SARS-CoV属于巢状病毒目、冠状病毒科,为单正链RNA病毒,基因组全长约29.7kb,已知编码11种蛋白,其中包括4种结构蛋白,分别是刺突蛋白、包膜蛋白、小包膜蛋白和核蛋白或核壳体蛋白。S蛋白三聚体形成突起,伸出包膜表面。S蛋白是病毒的主要表面抗原成分,具有受体结合和膜融合活性,在组织嗜性、细胞融合和毒力等方面起重要作用。N蛋白与基因组RNA结合形成核衣壳。M蛋白和E蛋白是包膜形成所必需的成分。

This study adopted Logistic Regression Mode by using ○ bank 2005-2006 pass personal consume fiduciary loan case as sample. Classify risk feature variables from 11 select items. The result discovered that the important redit risk variable for personal consume fiduciary loan customers to break contract is age, service seniority, annual income, personally debt……etc.

本研究采用罗吉斯回归模型,以○银2005年至2006年核贷之个人消费信用贷款案件为样本,选取十一项具代表性之风险特徵变做为分,实证结果发现,影响个人消费信用贷款核贷户违约之重要信用风险变数,为龄、服务资、年收入、本人负债情形等。

The present invention discloses beet noctuid nuclear polyhedrosis virus insect killing suspending agent, in which the virus bearer content is 5-20 hundred million PIB/ml., itis composed of chlorbenzuron analogue chlorfluazuron, aromatic amino acid tyrosine, fluorescent whitener, dispersant, glycerin, sterilized water.

本发明公开了一种甜菜夜蛾核多角体病毒杀虫悬浮剂。该剂型的主要成分是甜菜夜蛾核多角体病毒,悬浮剂中病毒包涵体含量5-20亿PIB/ml,它由灭幼脲类似物氟啶脲、芳香族氨基酸酪氨酸、精制荧光增白剂、分散剂、甘油、无菌水组成。

One problem which should be solved is how to determine window width when theories and methods of nonparametric kernel density are employed to simulate stand structures,which is also the core of applying the nonparametric kernel density estimation in practice and directly related to simulation precision.

应用非参数核密度估计理论和方法,对林分结构进行模拟中,一个必须解决的问题就是窗宽的确定,这也是非参数核密度估计在实际应用中的核心问题,它直接影响模拟精度。

Faba root tip was studied through detection of micronucleus technique Both of nitrate (0.08 mol/L) and nitrite (0.80 mmol/L) with low concn of could significantly induce the formation of micronucleus of V. faba root tip (P.01), and showed an obvious dose effect, i.e. the permillage of micronucleus was in linear increase with rising up of mutagen concn within a certain range.

结果]低浓度的硝酸盐(0.08mol/L)、亚硝酸盐(0.80mmol/L)均能显著诱发蚕豆根尖微核的形成(P.01),并呈现出明显的剂量效应,即微核千分率在一定范围内随诱变剂浓度的增加而线性增加。

She started to receive traditional Chinese medicine treatment on May 17 2008. Hodgkin's lymphoma falls into the area of scrofula, phlegm nodule, or tuberculosis of lymph node in TCM, and the mechanism is phlegm obstruction transforming into fire with stagnation of qi and blood, deficiency of liver and kidney and deficiency of both qi and yin.

本病是属於中医"痰核"、"瘰癧"、"恶核"等范畴,其病机为痰凝化火加上气血瘀结为患,就中医辨证分型而言,其病位在脾肝肾,病机在肝肾亏损、气阴两虚、痰凝兼有血瘀,并出现阴虚有热之证,秦艽鳖甲散可滋阴清热,软坚散结故作为本病主方,配合一贯煎来平补肝肾之阴,再以沙参麦冬汤来养阴生津清热。

As to Balilla erect panicle type,(the same subject for the following 11-16), the curvature of panicle neck had a very significantly positive correlation with panicle length, neck panicle length, neck panicle internode length, the secondary internode length, leaf length and leaf sheath length, and had an opposite correlation with leaf width and density of seed setting.

来自吉林的直立穗型品系Z1和来自黑龙江的直立穗型品种龙交91060—2与巴利拉型直立穗型品种的直立穗其因分属于3个不同的位点,各自位于3对同源染色体上,彼此遵循基因的自由重组和独立分配规律。Z1由1对显性主效核基因控制,龙交91061—2由1对隐性主效核基因控制。3个直立穗型基因位点可能具有累加效应,直立穗型效应位点越多,则颈穗弯曲度越小,穗的直立性越强。

Linked quadrilateral pyramid and hexangular pyramid as a precursor nucleus,two new microcrystals of silver bromoiodide were prepared.

本文研究了用连体四角锥碘化银为晶核制备类似于四面体碘溴化银微晶和用六方角锥碘化银为晶核制备片状碘溴化银微晶的方法,并对两种不同微晶的结构,形态和性能进行了比较和分

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