分枝的

In this experiment, we screen the major protective antigen gene-SOD gene of M. paratuberculosisin order to study the sensitive, specific diagnostic reagent and prophylaxis preparation, especially theDNA vaccine. The SOD gene was amplified from Mycobacterium paratuberculosis C-2 chromosomalDNA by using the PCR technique and cloned into pMD18-T Vector System. We gained a SOD gene of624bp.The recombinant clone was identified byα-complementarity, enzyme digestion and PCRidentification. The result indicated that the recombinant plasmid pMD18-T-SOD was successfullyconstructed. Moreover, through sequential determination and DNASTAR analysis between the clonedSOD gene of M. paratuberculosis C-2 and that of the M.paratuberculosis K-10 strain, the sequentialhomogeneity reached 99%, and the amino acid homogeneity reached 99.5%. The preceding analysisindicated that the SOD gene was very conservative in M. paratuberculosis.

为了研制副结核病敏感、特异的诊断试剂和新型、高效的预防制剂，尤其是DNA疫苗，本研究筛选了M.paratuberculosis主要保护性抗原SOD基因，以M.paratuberculosis C-2染色体DNA为模板，以SOD基因的特异性引物进行PCR扩增，获得了624bp的SOD基因，通过T-A克隆技术，将PCR产物克隆至pMD18-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pMD18-T-SOD，序列测定及DNASTAR分析表明，所获得的M.paratuberculosis C-2 SOD基因与Gen Bank中M.paratuberculosis K-10 SOD基因的大小完全一致，两者核苷酸序列的同源性为99％，氨基酸序列的同源性为99.5％，表明该基因在副结核分枝杆菌中是高度保守的。

Tricholoma may be a paraphyletic genus two. The collections identified as T. caligatum is a mixture. Some parts of them from France are members of T. matsutake, the other parts from North America are closed to T. bakamatsutake and T. fulvocastaneum. Tricholoma robustum and T. focale form a clade which is obviously a sister group of the stirp Subannulate. The stirp Caligata was defined as a group mainly on the basis of the predominant veil. But from the result of molecular systematic analysis, the presence of veil is not a stable character in the matsutake group. Species having symbiosis with conifers and broad leaf tree are all transformed from those whose host specificity is not strict. The phylogenetic tree based on ITS sequences resembled the tree of NJ algorithm based on cladistic coding. In the phylogenetic tree based on morphylogical characters, T. zangii and T. bakamatsutake form a clade, but it is the result of convergent evolution as suggested by molecular analyses. The distribution area of the matsutake group can be divided into six regions: middle and northern Europe region, Mediterranean region, Hengduan Mount.— Southeastern Tibet region, Northeastern China—Japan—Far-east Russia region, the Atlantic—North America region and the Rocky Mountain region. The abundance center of the matsutake group are Hengduan Mount.—Southeastern Tibet region and the Rocky Mountain region. Hengduan Mount.

松口蘑群的分子系统学研究得出的结果有：由于粗壮口蘑和羽衣口蘑明显和亚环族(Tricholoma，section subannulate)的种类是来自同一个谱系，和其它松口蘑群的种不具有最近共同祖先，因此松口蘑群是一个多系群；松口蘑群和口蘑属其他种类的亲缘关系比和蜜环菌属更接近；梭柄松苞菇和松口蘑群的差距明显小于油黄口蘑(T.flavovirens)和棕灰口蘑；口蘑属是一个多系群；鉴定为欧洲口蘑的标本明显不是一个单系群，其中一部分是松口蘑，另一部分和黄褐口蘑及傻松口蘑(T.bakamatsutake)比较接近；划分松口蘑群的标准是菌环的存在，但从分子系统学的结果来看，菌环的存在与否并非是一个很稳定的性状；和针叶树专性共生及和阔叶树专性共生均由寄主专化性不强的种类转化而来；在形态学系统发育树上青冈口蘑和傻松口蘑被聚为一个分枝，分子系统学的研究结果表明，这是趋同进化的结果；用支序方法编码作出的NJ树的结果更接近ITS序列的结果。

After two-cluster testing,branch length testing and relative rate testing ,two sequences(Melantitta nigra and Aythya americana) of 15 Cty b sequences were deleted because thek evolutionary rate were heterogeneous. The divergence between Anseriformes and Galliformes was chosen as an anchor point. Nucleotide sequence and amino acid sequence of 13 sequences data set was used to calibrated Anatidae molecular clocks. The results indicated that amino acid sequence is better than nucleotide sequence when divergence time is calculated with two kinds of sequences. The reliable divergence time of Anatidae were estimated with """"1+2TiTv"""" model.

经过二簇检验、分枝长度检验和相对速率检验，删除2条速率不恒定的序列后，用剩余的13条序列，以鸡形目与雁形目的分歧时间(89.8MYA)为锚定点，用顺序标定法标定鸭科鸟类分子钟的分析结果表明，用&1+2TiTv&分析得到的鸭科鸟类的分歧时间比较可靠；氨基酸序列用泊松校正模型和Γ模型估计的分类单元间的分歧时间则比较一致，表明氨基酸序列更适合用于鸭科鸟类分子钟的研究；用同一基因标定分子钟时，较长序列得到的结果标准误比较小；与核苷酸序列相比，氨基酸序列更适于用作分子钟的标定。

The results showed that plant height, branching number, dry matter weight and uptake of B and Mo of Chamaecrista rotundifolia were increased by application of 1.0, 3.0, 7.0 mgkg^(-1) of boric acid or 0.5, 1.0, 4.0 mgkg^(-1) ammonium molybdate, the stabilities of cell wall, cell membrane and chloroplast membrane structure enhanced, the number of chloroplast increased, and the best effects were observed by the application of 3.0 mgkg^(-1) boric acid or 4.0 mgkg^(-1) ammonium molybdate.

结果表明，施用1.0、3.0、7.0mgkg^(-1)的硼酸或0.5、1.0、4.0mgkg^(-1)的钼酸铵都能增加圆叶决明的株高、分枝数、干物质重和硼／钼元素的吸收，稳定细胞壁、细胞膜、叶绿体膜的结构，增加叶绿体的数量；其中以3.0mgkg^(-1)的硼酸或4.0mgkg^(-1)的钼酸铵为最佳施用量。

By means of GPC,IR,GC-MS,~(13)CNMR,~1HNMR,Methylation analysisetc,structural properties of PST-1 were identified as follows:The Mwof PST-1 was 3.44×10~6 Da and its optical rotation was _D~(20)=+0.110°(c0.1, H_2O); PST-1 constituted 8 simple sugars and the molar ratio was 2,4-Dimethoxy-Mannose:Rhamnose:Ara-binose:Xylose:Galactose:D-Galacturonic acid:Mannose:D-glucuronic acid=2%:5%:24%:9%:3%:1%:46%:10%;The chief bone of PST-1 was 1,3,6-linked-β-D-Man residue and the side chains contained Furanoid and Pyranoid residues.

结合GPC、旋光度测定、IR、GC-MS、~(13)CNMR、~1HNMR、高碘酸氧化法、Smith降解以及甲基化方法等分析测试方法，得到PST-1的单糖组成及结构表征，实验结果如下：红豆杉多糖PST-1是重均分子量为3.44×10~6 Da的支链多糖，旋光度为20D=+0.110~0(c0.1，H_2O)；PST-1单糖组成为：2，4-Dimethoxy-Mannose：Rhamnose：Arabinose：Xylose：Galactose：D-Galacturonic acid：Mannose：D-glucuronic acid=2％：5％：24％：9％：3％：1％：46％：10％；PST-1的骨架结构为：具有1，3，6-连接的β-D-甘露糖残基骨架，侧链分枝包括非还原末端的呋喃型α-L-阿拉伯糖残基、吡喃型α-L-阿拉伯糖残基、β-D-木糖残基、β-D-甘露糖残基、2，4-二氧甲基-β-D-甘露糖残基和α-D-葡萄糖醛酸残基；侧链的糖残基也可能存在2，5-二氧-取代呋喃型α-L-阿拉伯糖基、3-氧-取代的β-D-木糖残基、6-氧-取代的α-D-半乳糖醛酸残基、6-氧-取代的α-D-半乳糖残基、4-氧-取代的α-D-葡萄糖醛酸残基和2-氧-取代的α-L-鼠李糖残基，同时后者也可能穿插在主链上。

Stipules green, falcate, rarely ovate or ovate-lanceolate, herbaceous, margin sharply serrate or lobed, rarely entire, apex acute or acuminate; petiole sparsely pilose or pubescent; leaf blade interrupted imparipinnate with (2 or)3 or 4 pairs of leaflets, reduced to 3 leaflets on upper leaves; leaflets sessile or shortly petiolulate, obovate, obovate-elliptic, or obovate-lanceolate, 1.5–5 × 1–2.5 cm, abaxially appressed pilose on veins, or densely pubescent or tomentose-pubescent between veins, rarely glabrescent, markedly or sparsely glandular punctate, adaxially pilose, or hirsute or hirtellous on veins, rarely glabrescent, base cuneate to broadly so, margin acutely to obtusely serrate, apex rounded to acute, rarely acuminate. Inflorescence terminal, spicate-racemose, branched or not; rachis pilose.

绿色，镰刀形的托叶，卵形或卵状披针形的很少，草本，边缘锐切成锯齿状或者有叶，很少全缘，先端锐尖或渐尖；叶柄疏生柔毛或短柔毛；叶片间断奇数羽状具(2或)3或4小叶，退化至3小叶在上部叶片上；短的小叶无柄或具小叶柄，倒卵形，倒卵状椭圆形，披针形或倒卵形， 1.5-5 * 1-2.5 厘米，背面的贴伏的具柔毛的在脉上，在脉之间的或密被短柔毛的或被绒毛的短柔毛，很少脱落无毛显著具点，正面具柔毛的或疏生腺体，或具粗毛或在脉上，很少脱落无毛，基部楔形到宽楔形，花序顶生，穗状总状，与否的分枝；轴具柔毛。

Inflorescence a raceme with 2 or 3(–6) spikelets, rarely a panicle with many spikelets, peduncle, axis, branches, and pedicels slender, glabrous, scabrous or pubescent along longitudinal angles, branches usually subtended by a scaly bract at base. Spikelets ± compressed at maturity, several to many florets; rachilla disarticulating below fertile florets, extended beyond palea of uppermost floret, internodes flattened or concave on side facing floret.

花序为一总状花序具2或3(-6)小穗，一圆锥花序具很多小穗，花序梗，轴，分枝和花梗纤细，沿着纵向的有棱的无毛，粗糙的或短柔毛的很少，成熟时的小穗多少压扁，数枚到多数小花；在肥沃的小花下面脱节的小穗轴，在最重要的小花的内稃以外延长，在边面对小花上节间被变平或者凹。

The numerous macro-plants during the Doushantuoian were branching and semi-floated and erected on seafloor. They advanced the photosynthetic efficiency and increase oxygen in water to improve in environment for metazoans; and they served as a primary producer in the paleoecosystem to provide an alternative and new food of metazoan.

陡山沱期的宏体藻类多分枝并直立固着于海底，提高了光合作用的效率，增加了海中水体中的含氧量，不仅改善了环境，为依赖一定氧而生活、生长和繁殖的后生动物给予了必要的支持，而且作为生态系统中的必要基础，为宏体后生动物的快速发展和演化提供了一个新的食物源。

Perhaps the short-day treatment of SD is necessary to the fertility.(2): Compared the 88-428 SD(14.8-15.2h/d) with the 88-428 LD(14.9-15.3h/d), a new isozyme band of Rf=0.19 was found for the first time in the leaves of the 88-428 SD from the branching.

大豆光敏雄性不育材料88-428在出苗后经过连续20天的短光照处理后，与长光照条件下的88-428相比，从分枝期起多了Rf=0.19的酶带，特别值得指出的是Rf=0.19的酶带在以后的发育时期内一直存在，说明经过短光照的诱导以后产生了新的物质，而且这种物质可以在细胞间运输，影响到植株的生殖生长。

Dead branches or knots in different sizes of tree was studied and different branch development phases of growth,suppression,death and self-pruning was analyzed to evaluate the effect that knot size and distribution impact on timber quality.Branch and knot dynamic models were developed using stand and tree variables.Based on multipleunit stepwise regression method, the individual tree growth model was established using forest and crown factors as the variable. The best taper equation model of Larix olgensis was bucking data of 130 sample trees selected in the studied area.

本研究以不同年龄、不同密度及不同立地条件的落叶松人工林作为研究对象，基于19块标准地中95株标准木的树干解析、枝解析及节子剖析数据，揭示了不同林分条件下林木的枝条数量及在树冠内的空间分布格局、生长发育规律；研究了不同大小树木的死枝和节子大小及其变化规律，分析了枝条的生长、被压、寿命和自然整枝过程，评价了节子大小和分布对木材质量的影响；以林分变量、林木变量和着枝深度等为自变量建立枝条生长和节子大小的动态预测模型；选择林分因子和林木的树冠因子作为竞争因子，用多元逐步回归的方法建立落叶松人工林与距离无关的单木生长模型，并根据研究地区选取的130株造材样木数据，建立了落叶松人工林最佳削度方程。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。