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Methods The total proteins of untreated and INH-treated mc^2 155 cells were separated by ampholyte pH gradient-based two-dimensional gel electrophoresis (2-DE). Tire differential expression proteins were analyzed by using image analysis software. Results Proteins of control group arid INH-treated group contained 159 and 221 protein spots, respectively.

观察不同浓度的异烟肼处理前后耻垢分枝杆菌生长曲线,确定药物处理SM细胞的最佳时间和浓度,并提取在最佳处理时间和浓度条件下的SM细胞总蛋白,采用载体两性电解质pH梯度双向凝胶电泳技术分离异烟肼处理前后耻垢分枝杆菌mc^2 155细胞的总蛋白质,用PDuest 7.3.1图像分析软件比较分析,以识别差异表达的蛋白质。

Our AFM study shows that both the compact and ramified Ag clusters observed by the optical microscope are composed of smaller atomic clusters (~50 nm).

原子力显微镜表面分析结果显示:用光学显微镜所观察到的准圆形团簇和分枝状凝聚体的分枝均是由一颗颗更小(~50nm)的团簇组成的。

Leaf sheaths shorter than internodes, loose, pilose or glabrescent; leaf blades linear, flat or convolute, rigid, 15–30 cm × 5–8 mm, glabrous or hairy, apex finely acuminate; ligule 0.3–0.5 mm. Panicle usually contracted, dense, infrequently looser, 15–35 cm; central axis and branches scabrid-hispidulous; branches 2.5–10 cm, fasciculate in middle part, ascending; pedicels scabrid, apex often with stiff bristles.

叶鞘短于节间,松散,具柔毛的或后脱落;叶片线形,平或,硬质,15-30厘米×5-8毫米,无毛或有毛,好渐尖的先端;叶舌0.3-0.5毫米圆锥花序通常签合同,浓,很少发生松散,15-35厘米;粗糙主轴线和分枝具短硬毛;分枝2.5-10厘米,束状的在中部,上升;粗糙的花梗,顶经常有硬的硬毛。

Methods From June 2005 to March 2008, treated 20 cases of the nontuberculous mycobacteria hand infection patients after the start of functional rehabilitation treatment time were divided into Group A, B, each of 10 cases, both surgical debridement and anti-Mycobacterium therapy, rewirable after the first day until the wound for wound healing time, follow-up to 6 months after the operation, observe the wound healing time and the recurrence; according to the joint activity score standard score comparison group A, B group of before and after six months of the functional differences.

对2005年6月~2008年3月收治的20例手部非结核分枝杆菌感染患者按术后开始进行功能康复治疗的时间随机分为A组(术后1周)、B组(术后3周),每组10例,均行手术清除病灶和抗分枝杆菌治疗,以术后第一日起至伤口拆线为伤口愈合时间,随访到术后6个月,观察伤口愈合时间、复发情况;根据关节活动度评分标准评分比较A组、B组术前与术后6个月功能的差异。

We concluded that sputum cultures in the Taipei Hospital Branches for Communicable Disease Control revealed that 4.06% were multi-drug resistant strains of Mycobacterium tuberculosis and a very high percentage of multi-drug resistance in Nontuberculous Mycobacterium, which requires the attention of health workers.

研究分析结果显示台北市立联合医院疾病管制院区所培养分离的菌株中结核分枝杆菌多重性抗药菌比例达4.06%,但非典型结核分枝杆菌抗药性之菌株比例却非常的高,值得密切的注意。

Methods Targeting the most common mutation types at codon 526 and codon 531 of Rifampiein Resistance Determining Region of rpoB gene from Mycobacterium tuberculosis ,labeled probes (526CAC, 526TAC, 531TCG and 531TTG) were designed to detect 38 Rifampin-resistant clinical isolates with known RRDR sequence, 24 Rifampin-sensitive clinical isolates with wild type sequence of RRDR and 5 nontuberculous mycobacteria isolates, then a method using real-time PCR was established.

方法针对结核分枝杆菌rpoB基因利福平耐药决定区(Rifampicin Resistance Determining Region,RRDR)526密码子和531密码子常见的突变形式设计探针(526CAC,526TAC,531TCG和531TTG),应用已知rpoB基因RRDR区序列的38株利福平耐药临床分离株和24株利福平敏感临床分离株以及5株非结核分枝杆菌菌株建立荧光定量PCR检测基因突变的方法。

All specimens are of small size, mostly less than 150 μm in diameter. In gross morphology, those problematical microfossils consist of stout tubes with closed base, tiny internal mold of elongated tube, parallel clustered unbranching tubes, spicule-like structure with expanded circular base, and branching tubes of various bifurcating styles.

作者在处理陕南末元古纪的化石过程中,发现了大量不同形态的微体管状化石(直径大多小于150μm),包括末端封闭的粗短管体,不分枝管体,末端扩展的骨针状构造及具多种分叉样式的分枝管体。

[Objective]To investigate the microbiostatic mechanism of ethambutol, proteomic methods were used to find differential expression proteins in Mycobacterium smegmatis mc2155 cells treated with ethambutol.

[目的]利用蛋白质组学方法,识别乙胺丁醇处理前后耻垢分枝杆菌mc2155细胞后差异表达的蛋白质,为进一步探讨乙胺丁醇抑制分枝杆菌生长的作用机制及寻找新的抗结核药物作用靶标奠定基础。

The growth functions of the plant root systems were used to control the increase of the length of internodes and branching axis and the increase of the branching angles.

生长函数用于控制内节点的长度、分枝根的长度和分枝角的增加。

Because the seedlings that live in gaps get light intermittent, overall bifurcation ratio are significantly lower than that under canopy and full sun.

在林隙中的幼苗受光的间歇性影响,总体分枝率明显小于全光、林冠下的幼苗分枝率。

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