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The stromas of them can be distinguished by the following features: the stromas of Cordyceps nigrolla has few branches and the largest diameter in these 8 kinds of Cordyceps while each stroma of Codyrceps sobolifera has branches and the diameter of stroma of each branchlet is comparatively smaller.

拟黑虫草和蝉花虫草的寄主相似,二者菌核最为粗大,但子座有所区分,拟黑虫草子座少有分枝,在这8种虫草中子座直径最大,蝉花虫草子座都有分枝,未见单枝者,且每一小枝子座直径较小

Respectively use chelex 100 resinate,boiling extraction,proteinase K digest/isopropanol precipitation,proteinase K digest/phenol/chloroformextra ction,alkaline lysis,guanidine thiocyanate lysis,guanidine hydrochloride lysis,spin column method to extract Mycobacterium tuberculosis DNA for multiplepolymerase chain eraction.

选择3份含结核分枝杆菌分别为4.325×103、6.857×104、5.356×105 Copies/ml的痰液,分别用Chelex 100树脂法、煮沸法、蛋白酶K消化异丙醇沉淀法、蛋白酶K消化酚/氯仿抽提法、碱裂解法、异硫氰酸胍裂解法、盐酸胍裂解法、离心柱法抽提结核分枝杆菌DNA作多重聚合酶链反应,显色法芯片检测结核分枝杆菌RFP和INH耐药基因。

Two supernumerary spikelet lines and two normal-spiked multispikeletlines of common wheattriticum aestivum L.

玉皮分枝麦和骊英3号分枝麦是稳定的穗分枝型普通小麦品系。

In addition, trichome of AtCK4 transgenic plants has only two branches or no branching; however, that of wild-type plants has three branches.

另外,野生型的叶片表皮毛为三个分枝,而转AtCK4基因植株的表皮毛多为两个分枝,甚至不分枝。

The main results were showed as follows:Through screen and identification of morphology in M_2 and M_3, respectively, the mutants with mutative traits of plant were found. In which, 11 mutations were leaf variations, including light-green leaf, deep-green leaf, durative yellowish leaf, temporary yellowish leaf, inlaid yellowish leaf, wrinkly leaf, smooth-edged curly leaf, splitting-shaped curly leaf, peduncular-shaped leaf, round leaf and larger leaf, 12 were mutations on plant type, including excessive branches, lacking branches, highness, dwarf-like, erect posture, reclinate posture, strong stalk, slender stalk, tufty branches, wrinkled plant, purple stalk and hair-covered plant, 14 were flower variations, including dense flowers, light-yellow petals, white petals, white-mosaic petals, wrinkled petales, back-rolled petals, narrow petals, larger petals, smaller petals, apetalous flowers, variable-numbered petals, fertile pistil protraction, sterile pistil protraction and multi-anther, and 5 were physiologic mutants, including genic male sterile, cytoplasmic male sterile, bud-dead, and early and later bloom.

主要结果如下:通过田间M_2筛选和M_3重复鉴定,在田间农艺性状方面共获得浅绿叶色、深绿叶色、转绿黄化叶、持续黄化叶、边缘黄化叶、皱缩叶、光叶型卷叶、裂叶型卷叶、薹叶形叶、宽圆叶和大叶等11种不同的叶部性状突变体;同时还筛选到多分枝、少分枝、高杆、矮杆、株型紧凑、株型松散、粗茎、细茎、丛生分枝、整株皱缩、紫茎、整株被毛等12种植株性状突变体,密花、浅色花瓣、白色花瓣、白斑花瓣、皱瓣、卷瓣、窄瓣、大瓣、小瓣、无瓣、花瓣数目不定、可育型柱头外露、不育型柱头外露和多雄蕊等14种花器性状突变体,以及细胞核雄性不育、细胞质雄性不育、死蕾、早花和迟花等5种生理性状突变体。

The results demonstrated that 22 of 50 were positive by PCR-ELISA method while only 7/50 and 18/50 by antiacid staining and culture respectively, and no positive result was found in 20 sputum specimens without Mycobacterium tuberculosis by the methods; Conclusion The pCR-ELISA method can objectively detect Mycobacterium tuberculosis from clinical specimens with high specificity and sensitivity.

对50份来自结核病人高度怀疑有结核分枝杆菌的标本检测表明,PCR-ELISA检出22份阳性,比抗酸染色法(7/50)、培养法(13/50)和PCR-电泳法(18/50)检出率高,而20份证实无结核分枝杆菌的标本,几种方法检查均为阴性。结论该法可敏感、特异和客观地检测临床标本中的结核分枝杆菌。

In order to investigate new methods to the detection of Mycobacterium tuberculosis and diagnostic value of FQ-PCR in the detection of DNA in Mycobacterium tuberculosis, FQ-PCR was used to detect DNA of Mycobacterium tuberculosis in body fluid from 68 patients with tuberculosis and 68 patients without tuberculosis and it was also compared with the two methods of smear acid-fast stainning and antituberculotic antibody.

为探讨结核分枝杆菌检测的新途径及其FQ-PCR技术在结核分枝杆菌DNA诊断中的实用价值,运用FQ-PCR技术对68例结核病患者和68例非结核病患者体液进行结核分枝杆菌DNA测定,并与涂片抗酸染色及ELISA—抗结核抗体法进行比较。

Trees evergreen; trunk monopodial, straight; branches nearly whorled; branchlets ridged and grooved, with each leaf borne on a persistent, peglike base; short branchlets absent; winter buds ovoid, conical, or subglobose, resinous or not, scales on terminal buds recurved or not, persistent at base of branchlets.

常绿乔木树干单轴分枝,直;分枝差不多轮生;小枝具脊和凹槽,有每叶生于一持久,钉状基础;短小枝无;卵球形,圆锥状的冬芽,或近球形,与否树脂,由顶芽开出的鳞片与否,宿存在基部的小枝。

The determination of differential proteins between Mycobacterium tuberculosis and nontuberculosis mycobaeteria may he helpful to differentiation of mycobacteria.

结核分枝杆菌和非结核分枝杆菌差异蛋白的发现,可能有助于分枝杆菌的鉴别。

ABSTRACT:Nine proteins encoded by Mycobacterium tuberculosis RD1 region are important protective antigens that become absent in long passaging of Mycobacterium tuberculosis. They only exist in pathogenic Mycobacteria and are absent in Bacille Calmette-Guerin and environmental Mycobacteria. With good immunogenicities, they may play an important role in the diagnosis and prevention of Mycobacterium tuberculosis.

RD1区编码的9个蛋白,是结核分枝杆菌在长期传代过程中丢失的重要保护性抗原,仅存在于致病性分枝杆菌中,在卡介苗及环境分枝杆菌中缺失;其有较强的免疫原性,在结核病的诊断中发挥重要作用。

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