分枝性

objective to study the curative effect of fulguration combining mycobacterium injection in treat ment of condyloma acuminatum.methods the patients with ca were treated by fulguration in large range and local mycobacterium injection under fulguration wound.results the recurrence rates were 20.59% and 58.82% respectively in the observation group and in the control group.

目的 探讨电灼联合局部注射草分枝杆菌治疗尖锐湿疣的效果。方法治疗组应用电灼大范围炭化疣体，并在创面即疣体的基底部注射草分枝杆菌。对照组单纯采用高频电灼治疗。结果两组愈后复发率分别为20.59%、58.82%，前者明显较后者低，两组比较差异有非常显著性（ p ＜0.01）。

It also can be used as alternative antigen of newgeneration vaccine.In this experiment,we screen the major protective antigen hsp65 gene of MAP in order to developnew vaccine especially the DNA vaccine for the prevention of paratuberculosis disease.The hsp65 genewas amplified from MAP C-2 chromosomal DNA by using the PCR technique.We gained a hsp65 gene of 1 626bp.Then PCR product was cloned into pGEM-T vector by T-A clone technique and therecombinant clone was identified by plasmid size,enzyme digestion and PCR identification.The cloneplasmid of pGEM-T- hsp65 was successfully constructed.The nucleotide sequence and deduced aminoacid sequence ofclone gene was analyzed by DNASTAR software.The result indicated that the size ofhsp65 gene consist with M.paratuberculosis K-10 strain in GenBank and the sequential homogeneityreached 99.1%,the amino acid homogeneity reached 99.3%.The preceding analysis indicated that thehsp65 gene was very conservative in M.paratuberculosis.

为了研发预防副结核病的新型疫苗尤其是DNA疫苗及相关蛋白功能，本研究选择了MAP的主要保护性抗原Hsp65蛋白，以副结核分枝杆菌C-2株染色体DNA为模板，以hsp65基因的特异性引物进行PCR扩增，获得了1 626bp的hsp65基因，通过T-A克隆技术，将PCR产物克隆至pGEM-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pGEM-T-hsp65，以DNASTAR软件分析了所克隆基因的核苷酸序列和推导的氨基酸序列，结果表明，所获得的hsp65基因与GenBank中MAPK-10株该基因核苷酸大小完全一致，两者核苷酸序列的同源性为99.1％，氨基酸序列的同源性为99.3％，表明该基因在副结核分枝杆菌中高度保守。

Specific features different from normal objects for visualization cover self-similarity, ramification, branch bending, phototaxy, smoothly and un-smoothly blending branching etc.

树木可视化还有不同于其他物体的特性，如多级分枝、枝条的弯曲、自相似、向光性、树木分枝间的光滑与非光滑连接等。

We observed marophages pre-treated with ConA spread more rapidly on the well bottom compared with PBS injected controls under phase-contrast microscope. SEM of macrophages treated with ConA showed marked larger size and many surface ridges or ruffles and fine fingerlike processes or filopodia in comparison to controls, surface of which were smooth.

结果表明：①ConA处理后，倒置显微镜下可见Mφ粘附性增强；扫描电子显微镜下见其体积增大，指状突起增多；透射电子显微镜下见Mφ内线粒体等细胞器增多，伸出伪足包绕结核分枝杆菌并内吞，ConA活化的Mφ吞噬结核分枝杆菌数量增多，菌体的完整性受损。

In this study we build a Mycobacterium marinum random mutant libratory with MycoMarT7 transposon phage, found two cell wall virulence genes with colony morphology and cording structure strategy and build up virulence detection system in cell and animal model. We concluded that PDIM is critical for the virulence of Mycobacterium marinum.

本课题利用MycoMarT7噬菌体建立了海分枝杆菌随机突变库，以海分枝杆菌为模型筛选出两个分枝杆菌细胞壁毒力相关基因，并建立了海分枝杆菌的毒力检测系统，发现PDIM在海分枝杆菌的致病性中起到重要重要的作用。

Morifolium is different in some botanic characters:"Hongxinju" was higher than other cultivars on height of plant, length of lamina, diameter of stem and agricultural character;"Xiaobaiju" is the best on the ramification and the quantity of capitulum on individual plant;"Dabaiju" is the most on the quantity and layer number of ligulate flower;"Changbanju" is me longest on the capitulum diameter and corolla of ligulate flower.2, The plant height, rate of dried material accumulate and chlorophyll content of four medicinal C.

本研究采用比较分析的方法，于2001年和2002年在同一栽培条件下，对江苏省射阳县多年栽培的药用白菊花种内分化的4个主要药用白菊花栽培类型，进行植物学形态、内在质量、苗期耐盐性、产量性状等方面进行了比较系统的研究，结果如下： 1、4个主要药用白菊花栽培类型在植物学性状方面存在一定的差异；红心菊株高最高，茎杆较粗，叶片最大，农艺性状较好；小白菊每株头状花序数最多，小白菊分枝性好；大白菊舌状花层数、数目最多；长瓣菊花序直径最大，舌状花花冠最长。

Results: Cultures for Mycobacterium were positive in a total of 2657 people, among them 1848 strains (69.55%) were Mycobacterium tuberculosis, and 809 strains (30.45%) were Nontuberculous Mycobacterium. Among the 1848 strains of Mycobacterium tuberculosis, 337 strains (18.24%) were drug resistant, and 75 strains (4.06%) of them were resistant to both Isoniazid and Rifampin which made them multi-drug resistant. Among the 548 strains of Nontuberculous Mycobacterium, 453 strains (82.66%) were drug resistant. There were a total of 261 rapidly growing mycobacteria, and 242 (92.72%) of them were drug resistant.

结果：分枝杆菌培养阳性共2657菌株/人，其中1848株(69.55%)为结核分枝杆菌，809株(30.45%)为非典型结核分枝杆菌感染。1848株结核分枝杆菌中，337株(18.24%)有抗药性问题，其中75株(4.06%)同时对Isoniazid和Rifampin有抗药性为多重性抗药菌。548株非典型结核分枝杆菌中453株(82.66%)有抗药性问题；快速生长菌群共261株，其中242株(92.72%)有抗药性问题。

We concluded that sputum cultures in the Taipei Hospital Branches for Communicable Disease Control revealed that 4.06% were multi-drug resistant strains of Mycobacterium tuberculosis and a very high percentage of multi-drug resistance in Nontuberculous Mycobacterium, which requires the attention of health workers.

研究分析结果显示台北市立联合医院疾病管制院区所培养分离的菌株中结核分枝杆菌多重性抗药菌比例达4.06%，但非典型结核分枝杆菌抗药性之菌株比例却非常的高，值得密切的注意。

ABSTRACT:Nine proteins encoded by Mycobacterium tuberculosis RD1 region are important protective antigens that become absent in long passaging of Mycobacterium tuberculosis. They only exist in pathogenic Mycobacteria and are absent in Bacille Calmette-Guerin and environmental Mycobacteria. With good immunogenicities, they may play an important role in the diagnosis and prevention of Mycobacterium tuberculosis.

RD1区编码的9个蛋白，是结核分枝杆菌在长期传代过程中丢失的重要保护性抗原，仅存在于致病性分枝杆菌中，在卡介苗及环境分枝杆菌中缺失；其有较强的免疫原性，在结核病的诊断中发挥重要作用。

To obligatory pathogens like Pseudoperonospron cubensis, it could not only hold back the infection of hypha,but also heal the spot where infected by hypha.(8)The study on mechanism of action showed that the crude extract of S-5210 had a strong inhibition of the mycelia growth and spore germination which led to some abnormal growth such as the twisted and inflated mycelia, the abnormal increased branching, the broken mycelia wall and leakage of cytoplasm from hyphal tips.Alao the spores and the rate of germination were decreased, the germ tube was malformed in shape et al.The pathogenicity of hypha and spore was weakened.

8S-5210 活性产物的拮抗机理初步研究表明：S-5210 粗提物能强烈抑制病原菌菌丝的生长和孢子的萌发，引起病原菌菌丝扭曲或膨大、分枝增多、分枝顶端细胞壁破裂、原生质外溢；分生孢子数减少，孢子萌发率降低，芽管畸形等异常现象；菌丝和分生孢子致病性减弱。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。