分析曲线

Adopting the model of uniform load and triangular load separately,MRFs of different story numbers and bay numbers are analyzed by static Pushover analytical method,and the global capacity envelopes are obtained.Then,through a method for establishing the inelastic demand spectrum of design intensity and rare intensity based on elastic spectrum of seismic design code for buildings,the structural target displacement of design intensity and rare intensity are estimated by improved capacity spectrum method.The corresponding structural ductility reduction factor,the overstrength factor,the structural influencing coefficient and displacement amplification factor are determined.

分别采用水平均匀分布力和倒三角分布力加载模式对不同层数和跨数的钢框架结构进行了静力推覆分析，并得到了结构的能力曲线，在此基础上，应用改进的能力谱法，以规范反应谱为基础建立设防烈度和罕遇烈度下的弹塑性需求谱，确定出结构在设防烈度和罕遇烈度下的目标位移，进而求解出结构的延性折减系数、超强系数、结构影响系数和位移放大系数等参数；同时，分析了不同加载模式、层数和跨数等对各系数的影响。

TyPe II collagen induced arthritisln the rat ank1e joint andoVathumin as antigen induced arthritis WA in the rabbit knee joint wereestab1ish2 Qualitative evaluation of me in skin, muscle, synovium, cedilagearound joint and blood was performed by OMA3 The CIA rats were treated on day 7 after hind paw swelling and erythemaAnimals were injected intravenously with ase at a dose of 10mg/kg,tWenty minuots 1ater, one ankle of the rats random1y assigned was exPosedlaser irradiation at l00J/cm fOr l000 seconds, and another ankle wasM grouP wihout laser The other two groups is unmanipulatedcontrol group and untreated CIA group Bimaleolar ankle widthmeasuremellts were taken in all animals every tWo days using amicrometer The histopathology of the ank1e Joint was assessed at day 21after disease onset4 The pro1iferating cell nuclear antigen WCNA of CIA treated by PDT andthe HMME group without laser was doterdrined by immunohistochemiStry5 The AfA rabbits were treated on day 7 after knee swelling and erythemaThe theraPy invo1ved lntravenous injection of l0mg/kg HMME, fOl1owedby 20 minues period in dim light, and transdermal light treatment with\l00 J/cm2 fOr l000 seconds The inner sides of the treated Anees wereirradiated at first, and then the outer side did 24 hours later, the synovialtissue of the Anees joint were removed and in situ cel1 aPoptosis wasdetCCted With tednal deoxync1eotidyl transferase-mediated dUTP nickend labelingR6suIt8:l The pathologic changes of CIA and AIA include subsynovial inflammation,opovial hyPerplasia, pannus formation, cartilage and bone destructionresemble RA.2 The studies demonstrated that there are different uptake of HMME withinskin, muscle, synovium, cartilage and b1ood, and the synovium cou1draPidly uPtake more ase than skin and cartilage at the firSt 30 minuesaller intravenous injection of HMME3 The bimaleolar anke width had no different among PDT treated group,H group withollt 1aser and untreated CIA group But hlstologicalevaluation showed statiStical1y significallt reductions in synovialhyperplasia, pannus formation and cart1lage reosion, bone destruction andtotal score in PDT treated group4 Image analysis showed that the ratlo bforeen the areas of the coufltedobect to that of the entire area in PDTtreated grOup is lower than that in conirol group, but the integrated oPticaldensity had no different between the two groups5 Imape analysis showed that the ratio between the area of the countedobject to that of the e

治疗组在大鼠出现踝关节红肿后1周，炎症达到高峰时进行PDT治疗。随机治疗大鼠一侧的踝关节，另。2。一一侧作单纯HMME 对照。治疗方法是大鼠麻醉后尾静脉注入 HMME10ngkg，20分钟后踝关节照光，激光波长627.sum，功率密度 100mwcm'，照射时间1000秒，能量密度100）／。治疗后避光喂养72 小时。隔日一次测量大鼠的踝关节左右横径，治疗后两周取关节进行病理d 观察。 4。大鼠CIA模型用上述方法进行PDT治疗后，治疗组和单纯HMME 组用兔疫组化SP法检测石蜡切片的核增殖抗原。 5。兔AIA模型在关节炎出现第七天进行PDT治疗，随机治疗一侧膝关节，另一侧作自身对照。兔耳静脉注入I'arrainrelomg／Kg，20分钟后，膝关节用金蒸气激光照射，激光能量密度100）儿旷。24 ／J'时后取膝关节滑膜作病理检查，并用脱氧核昔酸末端转移酶介导的缺口末端标记法原位检测凋亡细胞。结果： 1。模型观察：CIA大鼠炎症高峰期滑膜下炎细胞浸润明显，滑膜细胞明显增殖，炎症达到高峰后二周，血管缀形成，并侵蚀和破坏软骨和骨， CIA模型病理改变与人类RA相似。兔AIA模型膝关节滑膜病理可见滑膜细胞增生，滑膜下炎细胞浸润，也与人类RA滑膜改变相似。 2。关节周围组织中光敏剂含量的测定结果表明，各组织对HMME 的吸收速度和吸收量不同，荧光值一时间曲线不同，滑膜组织比皮肤和软骨对 HMME的吸收多，在 2 0分钟时即有明显差异。 3.PDT对CIA模型的治疗结果表明：PDT治疗后关节炎组、单纯 HMME组和治疗组踝关节左右横径统计学检验差异没有显著性，但病理评分PDT治疗组滑膜增生、血管资形成及软骨破坏、骨破坏和总分比关节炎对照组和HMME对照组好，统计学检验差异有显著性。。3＿军医进修学院硕士学位论文中文摘要 4.PDT治疗组PCNA阳性细胞较对照组少，图像分析结果表明面密度（阳性染色的面积总和与统计视野面积的比值）治疗组小于对照组，统计学检验差异有显著性。。 5.PDT治疗组凋亡阳性细胞较对照组明显增多，图像分析结果单位视野内阳性细胞数和面密度PDT治疗组高于对照组，统计学检验差异有显著性。凋亡细胞核直径PDT治疗组较小，与对照组相比，统计学检验差异有显著性。结论：二。CIA、AIA的病理改变类似人类RA，可作为研究RA病因、发病机制、检查及治疗方法的模型。 2。各组织对HMME的吸收速度和吸收量不同，滑膜组织比皮。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Ringy amorphous alloy Fe36Co36B20Si4Nb4 is obtained by the method of copper mould suction casting. Wholly amorphous structure is proved by x-ray diffraction and differential scanning calorimeter. The parameters obtained from DSC proves that it has high glass forming ability.

采用铜模吸铸法，制备出管状非晶态合金Fe36Co36B20Si4Nb4.X-射线衍射分析与差示扫描量热曲线DSC分析表明，Fe36Co36B20Si4Nb4完全是非晶态，具有很强的玻璃形成能力，并确定了它的热力学参数。

It is indicated that, the bending curve is not asymmetrical and there will not have moment of flexure on the top of the bending section. Based on a slantwise constant-thick beam which foot is fixed, and took deadweight of bending section to account, a theoretical differential equation of third order for buckling equilibrium state was established.

在分析了顺层斜坡地质原型变形形态的基础上，由考虑自重的阻滑端固定的斜置等厚弹性板梁模型，提出了以地质原型变形形态抽象出相应边界条件的分析方法，及理论上求解挠曲线的适定三阶微分方程。

In this research, the spectra of five typical varieties of yogurt were measured by a spectroradiometer. Then clustering analysis was made using principle component analysis. Artificial neural network with principle components as input was used to establish discrimination model.

研究中，采用光谱仪获得5种典型酸奶品种的光谱曲线，然后用主成分分析法对5种酸奶品种进行聚类分析，再结合人工神经网络技术建立模型进行品种鉴别。

Methods. PSF 1000 analyzer was used to measure retinal image quality for eye of 26 subjects (spherical ranged from –3.00 ~-6.00Ds, astigmatism ranged from –0.75~-3.00Dc) fitted with three forms of full correction in random order : unaided eye, with rigid gas permeable contact lenses and toric soft contact lenses. The modulation transfer function curve was observed, 12 points of the MTF curve ( equivalent 12 points of LogMar VA) was used for analysis. Results.

利用人眼点扩散函数分析系统（PSF-1000）检查和评估26例近视散光患者（年龄18~28岁，球镜–3.00DS~-6.00 DS，角膜散光-0.75DC ~-3.00DC）在裸眼、配戴散光软性角膜接触镜和透氧性硬性角膜接触镜三种全矫状态的光学成像质量，采用26眼屈光介质MTF曲线中的12个点（即分别等同于对数视力表4.0~5.1的12个点）进行比较和统计学分析，为了避免主观误差，三种矫正方式的先后顺序随机选择。

By analyzing the lithology, physical property and the logging response characteristics ofthe tuffaceous sands reservoir in Xing'anling Group of Hailar Basin, this text identified thetuffaceous sands with both crossplot and probability statistical analysis, and establisheddiscriminating standards.

本文通过对海拉尔盆地兴安岭群凝灰质砂岩储层岩性、物性及测井响应特征的分析，应用测井曲线交会图和概率统计分析两种方法分别进行凝灰质砂岩的识别，并建立其识别标准。

The results of this study indicate that: 1 the post–surcharge secant secondary compression index decrease with the increase in surcharge ratio; 2 the amended post-surcharge secant secondary compression index also decrease with the increase in surcharge ratio; 3 time at which secondary compression reappears increases with the increase in surcharge ratio; 4 the post-surcharge secondary compression strain rate under the same final load with surcharging and without surcharging have a linear logarithm to logarithm relationship.

根据研究的结果显示：1超载比愈大，后超载割线二次压缩指数所受到的折减愈大，表示对软弱粘土的二次压缩沉陷量改良效果愈好；2修正之后超载割线二次压缩指数亦随超载比的增加而折减；3二次压缩再现时间与超载比的关系显示，增加超载比会延迟二次压缩再现的时间并可将分析结果以一均值曲线表示； 4对后超载应变速率分析发现在相同最终载重，未受超载作用与受超载作用的二次压缩应变速率呈现一双对数的线性关系。

In this paper, purification of growth hormone receptor from ovine liver and identification of its biochemical character have been done.Firstly, by homogenizing, triturating, high-speed centrifuging, hypervelocity centrifuging and sucrose density grade centrifuging cell membrane of ovine liver was separated and its electron microscope slice wasprotein was disL~olved with TritonX-100,amL-thebinding activity between cell membrane or membrane protein and ~I-hGH using RRAwas measured, perfect saturated curve wasgained. Thirdly, specific binding of GILR protein was measured ,by SDSPAGE, its molecular weight is about 1 19KD.

本研究采取新鲜羯羊肝脏，匀浆、研磨，经高速离心，超速离心和蔗糖密度梯度离心，分离肝脏质膜，得到肝脏质膜的电镜切片：其次，用去垢剂TritonX-100增溶膜蛋白，利用放射受体分析法，测定细胞膜及膜蛋白与~(125)I-hGH的结合活性，均得到理想的饱和曲线；再次，将两点结合试验法与凝胶层析法结合检测到特异结合物，然后，经SDS-PAGE电泳分析，纯化后的样品呈现一条带，与标准分子量比较，得出分子量为119KD。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。