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Methods The 26th exon, the 30th exon and the 21st intron of gene GP Ⅱb in 110 individuals were amplified by polymorphism and sequenced to investigate whether there was linkage among the polymorphisms of the gene. Human platelet antigen-3 (HPA-3) gene frequency was detected by Fok 1 enzyme in 147 patients with hematologic diseases, and was compared with that in 110 normal individuals. Forty-four patients who received apheresis platelet transfusion repeatedly were randomly divided into the HPA-3 homotype group and the control group. The antibodies of the platelet were detected after 3 times of platelet transfusion.

聚合酶链反应-单链构型多态性分析检测110例正常人GP Ⅱb基因第26和30外显子(Exon 26, Exon 30)及21内含子(Intron21)基因多态性,进行基因序分析,研究这些基因多态性是否存在连锁关系;应用Fok1酶切法对147例血液病人人类血小板抗原-3(human platelet antigen-3, HPA-3)基因进行分型,并与110例正常人进行比较;将接受单采血小板输注的44例血液病人随机分为HPA-3同型输注组和对照组,血小板输注3次以后检则血小板抗体。

The study of Constitutional science must be from normative analysis constitution to constitutional interpretation, the reason is that normative analysis constitution has an insolvable tension between fact and norm, namely, value and fact, availability and actual effect, norm and interpretation.

中国宪法学50年之发展,自注释宪法到规范分析宪法,其方法论已经发生了具有转折性的置换,但宪法学研究之所以需要从规范分析宪法到宪法解释学,实乃规范分析宪法学在事实与规范之间始终存在着难以逾越的张力,从而必然会导致价值性与事实性、有效性与实效性以及规范性与解释性的分离与脱节之弊。

Based on English definition of the six properties which a relation R defined on a set A and utilizing the logical characteristic of implications, two aspects of ordered-pair and the matrix MR are considered, and the properties of the R is analyzed one by one. A few useful conclusions are summarized as determining the properey of the R. The following collections are also analyzed between reflexive and irreflexive, symmetric and antisymmetric, symmetric and asymmetric and among irreflexive, antisymmetric and asymmetric.

根据定义在一个集合A上的关系R的六个性质的英文定义,利用蕴含式的逻辑特点,从有序对和关系R的矩阵M两个角度对每个性质逐一进行分析,得出在判定关系R的性质时可用的结论,并且分析了自反性与非自反性之间、对称性与反对称性之间、对称性与非对称性之间、非自反性和反对称性以及非对称性之间的联系。

Objective To analyze the CT and MRI manifestations of oral carcinoma,and to evaluate the value of imaging technologies in the diagnosis of oral carcinoma,thus to explore the most reasonable imaging method Methods 46 cases with oral carcinoma,proved by surgery and pathology (2 lip tumors,8 carcinomas of the gingival ridge,5 carcinomas of the buccal mucosa,24 tongue carcinomas,5 carcinomas of the floor of the mouth,2 tumors of the hard palate),were analysed retrospectively32 cases were examined with CT and 14 cases with MRIImaging presentations were compared with surgery and pathology Results Of the 46 cases with oral carcinoma,22 were ulcerative lesion of infiltrative growth,24 were masslike lesion of extruded trowthOf the 32 cases examined with CT scan,27 were detected,5 were missed,the 4 missed cases were confirmed to be superficial ulcers by surgery and pathologyOf the 10 cases with bone destruction examined with CT scan,8 were manifested,2 were missedAnd of the 14 cases with MRI scan,all 14 were detected Conclusion CT and MRI scan are valuable for diagnosis of oral carcinomasThey can manifest the morphology and size of primary tumor,contiguous structures,bone invasion and cervical lymph node metastasisMRI is the optimal imaging method for superficial ulcerative lesion of infiltratibe growth

徐 锋,罗德红,唐威,欧阳汉,周纯武目的分析口腔癌的CT及MRI表现,评价影像检查在口腔癌诊断中的应用价值,探索合理的影像学检查方法。方法回顾性分析经手术及病理证实的口腔癌46例(唇癌2例,牙龈癌8例,颊黏膜癌5例,舌癌24例,口底癌5例,硬腭癌2例),其中32例行CT扫描,14例行MRI扫描,将影像表现与手术所见及病理结果进行对照分析。结果 46例口腔癌中,呈浸润性生长的溃疡型病变22例,外生性生长的肿物型病变24例。CT扫描32例,明确显示病变27例,遗漏病变5例,其中4例为表浅溃疡型病变。CT扫描病例中,10例有骨质侵犯,CT扫描准确评价8例,遗漏2例。MRI扫描14例,均准确评价肿瘤及其与周围结构的关系。结论 CT、MRI对口腔癌原发肿瘤的形态、大小、周围结构侵犯、颈部淋巴结转移的评价有重要价值。对于较小及呈浸润性生长的表浅溃疡型肿瘤,CT扫描尤其是CT平扫难以准确显示,应首选MRI。

Analyzing the DNA Polymorphism: 14 random primers were selected to analyze the DNA polymorphism of 13 species of Melampsora larici-populina, the result indicates: The isolates of MLP have resemblance and difference, thee differences reflect the DNA Polymorphism of them.But the Polymorphism is not related to their origin.the result is consistent with departed one.

2DNA多态性分析:用所选出的14条随机引物对13种落叶松-杨栅锈菌进行RAPD分析,发现落叶松—杨栅锈菌菌种之间既有一定相似性,又具有明显的遗传多态性且多态性与地理来源没有必然的相关性,这与以往的结论相一致。

Focusing on the PGE enrichment mechanism in the basic-ultrabasic intrusions in the Panxi rift, firstly, through the interpretation of TM photograph, data processing of regional geochemical exploration and geophysical prospecting results of gravity, magnetic force and seism, we analyzed the controlling effect of structure and magmatic movement on the PGE mineralization; Secondly, through the study of volcanic apparatus, petrography, measurement of comprehensive geochemical section, isotope tracing and dating of typical PGE-rich melanocratic rock series, we discussed the sources of the PGE-bearing mafic-ultramafic intrusions and basalts, their timing, evolvement sequences, the geochemical processes of magma crystallization and fractionation, and the relationship between magma evolution and structural development; Finally, through the analysis of PGE and chalcophile elements concentrations in the melanocratic rock series and the contrastive study with the Noril''sk magmatic Ni-Cu-PGE sulfide deposit, its related continental flood basalts and Siberia Traps, we discussed PGE mineralization characteristics and its enrichment mechanisms in the melanocratic rock series in the Panxi rift.

本项目首先围绕川西地区主要的PGE矿床及伴生矿的地质环境和有关的地质构造背景,通过TM卫片解译、区域化探和重、磁及地震物探资料分析,总体把握构造及岩浆活动对PGE成矿的控制作用;然后通过对典型富PGE暗色岩套的火山机构地质构造、岩石岩相学、地球化学、同位素定年和示踪等方面的工作,分析本区基性-超基性含矿岩体与喷出岩之间的成岩物质来源、侵入时代及演化序列,研究该区同一岩浆房分异结晶的地球化学过程及其随构造发展的耦合关系;最后通过对暗色岩套PGE地球化学剖面分析以及与俄罗斯诺里尔斯克陆内裂谷溢流型玄武岩中PGE矿化的岩石-构造环境对比研究,探讨该区暗色岩套中的矿化特点和富集部位及其PGE富集机制。

Furthermore, out of 497 fAFLP markers, 80 special bands were found to be able to distinguish the four groups from each other and may be applied for germplasm characterization and molecular assistant classification of Meretrix clam.4 Molecular classification of two species of Meretrix clam based on fAFLP and ITS sequences4.1 The results of fAFLP maker analysis of S, G and W showed that each group had their own specific loci among which there were 53 special loci in W group, much more than those of S group (14) and G group (21). Among the 53 loci, nine were all dominant loci. These unique loci could be taken as molecular markers to distinguish W from other groups. The genetic similarity indexes and distance matrix between S and G groups were 0.9585 and 0.0424 respectively, but the genetic similarity indexes and distance matrix between W group and S or G group was 0.7939 or 0.7941, and 0.2308 or 0.2305 respectively. The results revealed that significant difference existed between W and S or G groups in molecular genetic structure. The phylogenetic trees by the methods of UPGMA and NJ also indicated that S and G populations were very closely related, while W population was a relatively independent cluster, lying beyond the species which S and G belong to.4.2 The internal transcribed spacer region of the rDNA from S group, G group and W group were PCR amplified and sequenced. The results showed that the size of ITS ranged between 1266-1269bp in W group, while those in G and S groups were 1614bp and 1520bp respectively. The GC content ranged 62.32-62.62% in W group while it was 61.77% in G group. The genetic distances between three populations of W group were 0.001~0.003, but it was 0.110 or 0.147 respectively between W group and G group or S group. Phylogenetic trees by NJ method also showed that G group was very closely related to S group, while W group was a relatively independent cluster.

在457个总扩增位点中找出了53个W的特有位点,远多于S群体(14)和G(21)群体,而且在53个特有位点中有9个出现频率为100%的位点,这些位点可以作为区分其它2个群体的特征性标记;S– G群体特有的位点有112个,其中有4个位点出现频率为100%,可作为S– G群体区别于W群体的特征性标记。S群体和G群体间的遗传相似性系数为0.9585,遗传距离只有0.0424,在NJ和UPGMA法构建的亲缘关系的树状图上均首先聚在一起,说明二者的亲缘关系很近,应属于种内群体间的关系;而W与S和G的遗传相似性系数均较小(0.7939和0.7941),相对遗传距离很大而且十分相近(0.2308和0.2305),在亲缘关系树状图上单独分出一支,也表明W与S和G群体间的亲缘关系较远。4.2 ITS序列比较分析通过对白壳文蛤、山东文蛤和广西文蛤的ITS序列扩增电泳、PCR-RFLP分析和ITS序列分析发现,W的ITS序列长度在1266-1269 bp,而S和与G的ITS序列总长度分别为1520 bp和1614 bp;从ITS1和ITS2长度来看,W分别为739-741 bp和316-317 bp,S为895 bp和414 bp,G为987 bp和416 bp;而从ITS碱基组成来看,W的GC含量在62.32-62.62%之间,而G群体为61.77%。W的3个壳色不同群体间的遗传距离仅0.001、0.002和0.003,S与G群体间的遗传距离是0.010,说明W群体内变异很小,而S与G群体间已出现明显的遗传分化,但还均属于种内群体间的遗传变异;而W与G和S的遗传距离分别达到0.110、0.147,两个类群差异显著,已远超出种内群体间的遗传变异。

By dehydrochlorination reaction the poly[(2-methoxyl-5-octyloxy)phenylene vinylene] is synthesized and the material"s structure is characterized by nuclear magnetic resonance spectroscopy, infrared absorption spectrum, ultraviolet and visible spectrophotometry. At the meantime, the fluorescence spectrum is measured and the mechanism of fluorescence occurring is analyzed. Thermal Gravimetric Analyzer"s results show that the MO-PPV"s thermal stability is fine. Also the experiment of resolvable property indicates that the MO-PPV can well dissolve in chloroform. Through analyzing the film"s surface by Metaloscope and Atomic Force Microscope, the good filming is found.

通过脱氯缩合法合成了聚(2-甲氧基-5-辛氧基)对苯乙炔,并分别用核磁共振波谱法、红外吸收光谱法、紫外-可见分光光度法等对其进行了结构表征;测量了样品的荧光光谱,分析了荧光产生的机理;热失重仪分析了材料的热稳定性,研究表明MO-PPV材料具有良好的热稳定性;溶解性实验发现材料在氯仿中溶解性较好;金相显微镜和原子力显微镜研究了薄膜的表面形貌,发现该材料的成膜性良好;材料的导电性研究表明,固体材料在掺杂下才可以表现出导电特性,而配成溶液的材料导电性因溶剂的不同而不同,并随溶解时间的长短发生变化。

Based on the failure analysis of hot roll, this paper optimizedly adjusts alloy system and summarizes the experience of rolling wiresand improves ail processing properties. Then, it analyses the effects on some natural parameters such as alloy transfer coefficient, surfacing layers, welding current and arc voltage etc.. It confirms the fitting ageing technique. Also, it discusses the microscopic structural transformation back and forth ageing and the effects on hardness change of overlaying metal by some alloying elements including C, Cr, V, W, Mo, Ni, Mn, B, Re through OM, SEM and X-ray diffraction analyses. Finally the abrasive resistance, red hardness, thermal fatigue property and anti-high temperature oxidability are investigated in experimentation.

全文在热轧辊失效分析的基础上优化调整合金体系,总结了焊丝轧制的经验,改善各种工艺性能,分析了几个特性参数的影响,确定了适宜的时效工艺,并通过光学显微镜、扫描电镜和X-射线衍射对时效前后的显微组织转变以及部分合金元素如C、Cr、V、W、Mo、Ni、Mn、B、Re对堆焊层硬度变化的影响进行分析,最后通过试验考察了堆焊层金属的耐磨粒磨损性能、红硬性、耐热疲劳性和抗高温氧化性。

ABSTRACT Objective To discuss the clinical pathologic features and differential diagnosis of mixed germ cell sex cord stromal tumorwith malignant variant of germ cell tumor Methods The clinical pathologic datum and immunophenotype were studied in the case of ovary MGCSCST with malignant variance of germ cell tumor Results The patient's genital anatomy and female phenotype were normal,karotypes was 46xx with uterogestation The tumor tissue consisted of mixed germ cells and sex cord stromal cells with evident malignancy of mixed germ cells There was no atypical structure of gonadoblastoma in tumor tissue Conclusion MGCSCSST has complex morphosis,thus,to prevent misdiagnosis,more samples should be collected and more slices be cut The patient may have prognosis malo when there are high malignant variants of mixed germ cells in tumor tissue

目的 探讨混合性生殖细胞性索间质肿瘤(mixed germ cell sex cord stromal tumor,MGCSCST)伴恶性生殖细胞肿瘤变异型的临床病理特点及鉴别诊断。方法卵巢MGCSCST伴恶性生殖细胞肿瘤变异型的病例进行临床病理特征和免疫表型分析。结果患者生殖器的解剖结构和女性表型正常,染色体组型是46xx,足月妊娠。肿瘤组织由混合性生殖细胞和性索间质肿瘤构成,混合性生殖细胞有显著的恶性特征。瘤组织中没有典型的性腺母细胞瘤结构。结论 MGCSCST有复杂的形态结构,因此更多的取材和切片可以防止误诊,当肿瘤组织中的混合性生殖细胞有高度恶性变异时,患者预后不良。卵巢肿瘤;生殖细胞性索间质肿瘤;免疫组织化学

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。