分析性

In this experiment, we screen the major protective antigen gene-SOD gene of M. paratuberculosisin order to study the sensitive, specific diagnostic reagent and prophylaxis preparation, especially theDNA vaccine. The SOD gene was amplified from Mycobacterium paratuberculosis C-2 chromosomalDNA by using the PCR technique and cloned into pMD18-T Vector System. We gained a SOD gene of624bp.The recombinant clone was identified byα-complementarity, enzyme digestion and PCRidentification. The result indicated that the recombinant plasmid pMD18-T-SOD was successfullyconstructed. Moreover, through sequential determination and DNASTAR analysis between the clonedSOD gene of M. paratuberculosis C-2 and that of the M.paratuberculosis K-10 strain, the sequentialhomogeneity reached 99%, and the amino acid homogeneity reached 99.5%. The preceding analysisindicated that the SOD gene was very conservative in M. paratuberculosis.

为了研制副结核病敏感、特异的诊断试剂和新型、高效的预防制剂，尤其是DNA疫苗，本研究筛选了M.paratuberculosis主要保护性抗原SOD基因，以M.paratuberculosis C-2染色体DNA为模板，以SOD基因的特异性引物进行PCR扩增，获得了624bp的SOD基因，通过T-A克隆技术，将PCR产物克隆至pMD18-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pMD18-T-SOD，序列测定及DNASTAR分析表明，所获得的M.paratuberculosis C-2 SOD基因与Gen Bank中M.paratuberculosis K-10 SOD基因的大小完全一致，两者核苷酸序列的同源性为99％，氨基酸序列的同源性为99.5％，表明该基因在副结核分枝杆菌中是高度保守的。

It also can be used as alternative antigen of newgeneration vaccine.In this experiment,we screen the major protective antigen hsp65 gene of MAP in order to developnew vaccine especially the DNA vaccine for the prevention of paratuberculosis disease.The hsp65 genewas amplified from MAP C-2 chromosomal DNA by using the PCR technique.We gained a hsp65 gene of 1 626bp.Then PCR product was cloned into pGEM-T vector by T-A clone technique and therecombinant clone was identified by plasmid size,enzyme digestion and PCR identification.The cloneplasmid of pGEM-T- hsp65 was successfully constructed.The nucleotide sequence and deduced aminoacid sequence ofclone gene was analyzed by DNASTAR software.The result indicated that the size ofhsp65 gene consist with M.paratuberculosis K-10 strain in GenBank and the sequential homogeneityreached 99.1%,the amino acid homogeneity reached 99.3%.The preceding analysis indicated that thehsp65 gene was very conservative in M.paratuberculosis.

为了研发预防副结核病的新型疫苗尤其是DNA疫苗及相关蛋白功能，本研究选择了MAP的主要保护性抗原Hsp65蛋白，以副结核分枝杆菌C-2株染色体DNA为模板，以hsp65基因的特异性引物进行PCR扩增，获得了1 626bp的hsp65基因，通过T-A克隆技术，将PCR产物克隆至pGEM-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pGEM-T-hsp65，以DNASTAR软件分析了所克隆基因的核苷酸序列和推导的氨基酸序列，结果表明，所获得的hsp65基因与GenBank中MAPK-10株该基因核苷酸大小完全一致，两者核苷酸序列的同源性为99.1％，氨基酸序列的同源性为99.3％，表明该基因在副结核分枝杆菌中高度保守。

In this paper, the petrology, mineralogy and geochemistry of basic-ultrabasic volcanic rock, granite and skarn related to the Sn-Cu skarn-type ore and the vein-type ore in Laochang ore field have been researched systematically, some conclusions are summarized as follows:In Gejiu tin-polymetallic district, the middle Triassic volcanic rocks belonging to calc-alkaline basalt-tholeiite serie, mainly consist of pyroxene, hornblende, feldspar, chlorite and phlogopite; All the granites are peraluminous granites related to the Yanshanian collision-orogeny. Laoka granite closely related to the tin-polymetallic deposits emplaced in the post- orogenic.The metal elements show obviously vertical zones with the variety of skarn types in dimensional extension; The occurrence of garnet skarn and diopside skarn alternately developed in the horizontal zones of the contact between granite and carbonatite are mainly related to two factors: one factor is the infiltration metasomatism of post-magmatic hydrothermal solution, the another is the metrical strata of marble and calcic-dolomite developed in country rocks.

本文重点以老厂矿田中的接触带锡铜矿床和脉状矿床为研究对象，采用化学分析、ICP-MS、X射线粉晶衍射分析、电子探针等测试手段，对本区与成矿相关的基性-超基性火山岩、花岗岩、夕卡岩、电气石-石榴石等进行了系统的地质地球化学研究，研究表明：个旧矿区中三叠纪个旧组地层中的火山岩为钙碱性玄武岩-拉斑玄武岩系列，岩石为辉石-角闪石-长石-绿泥石-金云母组合；本区的花岗岩为过铝质花岗岩，主要与燕山期碰撞造山作用有关，锡多金属成矿与形成时代最晚、分异较好的花岗岩关系密切；本区夕卡岩中的有用共生组份随着夕卡岩类型及其矿物组合的不同具有明显的分带性。

The measurement system error model analysis is introduced into power system real time network state analysis as an important part for the first time, which can improve on the state estimation quality and provide the capability to monitor the operation of the measurement system; 2. The theory and algorithm of the on-line estimation and update of measurement noise variance based on the relation between the residual variance and noise variance. The statistic properties of the sample variance are discussed and the relation between the estimation precision and sample size under given confidence level is derived; 3. The theory and algorithm of detection and identification of measurement bias are presented, which is based on the relation between residual mean and noise mean. The statistic properties of sample mean are discussed and the relation between estimation precision and sample size is derived; 4. The Givens orthogonal transformation algorithm is selected to be the essential algorithm of state estimation, the fast orthogonal transformation algorithm with damp factor and the algorithm which can handle the zero injection measurements efficiently are presented; 5. The quantity analysis theory of bad data detectivity and identifibility are presented, which describes the relation between the elements in matrix W〓 and bad data amplitude and can provide the theory base for measurement system design and valuation.

一、首次将量测系统误差模型分析做为一个环节引入电力系统实时网络状态分析中，为EMS系统增加了实时监视系统运行、修正量测系统误差模型的新功能，进一步发挥了实时网络状态分析应用软件的潜力；二、首次提出了应用样本方差在线估计与修正量测系统误差方差的基本理论，讨论了样本方差的统计性质和概率分布，推导出了样本容量、估计精度和置信度之间的关系，给出了在线估计与修正量测系统误差方差的算法；三、首次提出了应用样本均值在线检测与辨识量测偏差的基本理论，讨论了样本均值的统计性质，推导了样本容量、估计精度和置信度之间的基本关系，给出了在线检测与辨识量测偏差的算法；四、在状态估计算法设计中，以Givens变换算法做为基本算法，提出了快速正交变换阻尼因子法和可以有效地处理零注入量测的混合法，并对实时应用中的一些问题进行了讨论；五、提出了不良数据可检测性与可辨识性的定量分析理论，揭示了描述量测系统配置、网络结构与参数的残差灵敏度矩阵中的元素与不良数据的幅值在可检测、可辨识能力上的定量关系，为量测系统配置设计与评价提供了理论基础；六、综合国内外最新研究成果，采用自适应自回归预测技术和稀疏矢量技术，构造了较完善的不良数据检测与辨识算法。

The research is carried on from four aspects. One is, based on answering the above open problem on a finite dimensional Euclidean space by means of partially ordered theory, to research the existence of solutions, global error bounds of proximal solutions and sensitivity of parametric unique solutions and present a class of variable-parameter three-step iterative algorithms for generalized set-valued variational inclusion problems by using - resolvent operator of set-valued mapping.Two is to consider the convexity, closedness and boundedness of the solution set of general set-valued variational inclusion problems and the sensitivity of the parametric solution set by means of graphical convergence theory. Three is to discuss directly the existence of solutions by using analytical methods for set-valued mixed quasi-variational-like inequalities and suggest a class of direct variable-parameter three-stepiterative algorithms for solving generalized set-valued variational inclusions.

研究分有三个方面：一是借助于偏序理论在有限维欧氏空间中解决了上述公开问题，在此基础上利用集值映射的η-预解算子，研究了广义集值变分包含问题解的存在性、逼近解的全局误差界、参数唯一解的灵敏性，并提出了一类变参数三步迭代算法；二是借助于图收敛理论研究了一般集值变分包含问题解集的凸性、闭性和有界性以及参数解集的灵敏性；三是用分析的方法直接讨论了集值混合拟类变分不等式问题解的存在性并提出了一类求解广义集值变分包含问题的直接变参数三步迭代算法。

During this course, there are contradictions between modern and postmodern, struggle between rationalism and sensualism, antinomy between structuralism and deconstructionism, dissension between homogeneity and heterogeneity... These contradictions and dissension are the embodiment of transformation from industrial society to post-industrial society in the cultural domain. It provides references for the socialist culture construction of our country.

从文化学层面分析，它充斥了文化同质性与文化异质性的对抗与消解，文化高雅性与文化通俗性的对峙与融合，文化工具性与文化价值性的斗争与合流；从哲学层面分析，它又体现了文化一元主义与多元主义的对立与统一，文化结构主义与文化解构主义的交锋与并立，文化绝对主义与文化相对主义的冲突与共存，这一切集中反映了西方在由工业社会向后工业社会转变过程中文化的特有矛盾和状态，也为我国社会主义文化建设提供了借鉴和参考的价值。

An algorithm of cluster analysis with statistic significance test to classification was developed in this paper.

鉴于此，本研究用随机化方法对分类进行统计显著性检验，建立了具有统计显著性标记的聚类分析算法，用于对若干个样品进行有显著性标记的聚类分析。

Based on the test of 23-year and 10-year old slash pine,this papersystematically studied the effect of different thinning intensity and different densityof plantation on the wood qualities and properties.Many statistical means such asanalysis of variance and cluster analysis were used to analyze the difference of thewood qualities and properties among the different silvicultural measures.

本文分别以23年生、10年生的湿地松为实验材料，全面地研究了不同间伐强度、不同种植树密度对人工林湿地松材质材性的影响规律，并利用方差分析、聚类分析等统计手段，定量地分析了不同栽培措施之间材质、材性的差异。

AFLP marker technique was used to investigate the genetic relationships between 50 accessions of maize and its wild relatives including teosinte, Tripsacum and Coix With twenty one AFLP primer combinations of PstⅠ/MseⅠ, 641 AFLP amplified bands were obtained, including 586 polymorphic bands with an average of 30 52 per primer combination and the polymorphism percentage of 91 4% Cluster analysis using UPGMA method based on the data of AFLP showed that the 50 materials could be divided into...

用AFLP分子标记技术对 5 0个有代表性的玉米及其野生近缘种大刍草、摩擦禾、薏苡材料进行了遗传关系分析。利用 2 1个PstⅠ MseⅠ引物组合检出可辨认带共 6 4 1条，其中多态性带 5 86条，平均每对引物得到 30 5 2条多态性带，多态性 91 4 %。聚类分析结果表明，可以将 5 0个材料分为 3大类，与已有的玉米族分类一致，但与玉蜀黍属内的种间和种内分类存在差异，说明大刍草各种内存在较大的遗传多样性。本实验发现玉蜀黍属与摩擦禾关系比与薏苡的关系更近，但并没有发现玉米与小颖玉米亚种比与其他大刍草的遗传关系更近。

This paper presents a new method of the comprehensive parameter identification for real - time transient security analysis.

本文提出了一种应用综合参数识别进行电力系统实时暂态安全分析的新方法、用在电力系统实时暂态安全分析监测系统上，提高了暂态安全分析的实时性、通过对某一实际电力系统的仿真研究表明。这种方法具有好的实时性和实用性。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失