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METHODS: Fetal liver was obtained sterilely. Monoplast suspension was collected by collagenase digestion and mechanical separation, and then centrifuged using 1.070 g/mL Percoll separating medium and 1.077 g/mL Ficoll separating medium. Cells achieved from the interface of separatory liquids were cultured in DMEM/F12 medium, supplemented with 0.1 volume fraction of fetal bovine serum, 20 μg/L hepatocyte growth factor and 40 μg/L epidermal growth factor, for 9 days.

无菌状态下取出胎儿肝脏,以胶原酶消化法和机械分离法获得胎儿肝脏来源单细胞悬液,分别采用1.070 g/mL Percoll分离液和1.077 g/mL Ficoll分离液进行密度梯度离心,吸取界层细胞,添加含体积分数为0.1 FBS、20 μg/L肝细胞生长因子、40 μg/L表皮生长因子的DMEM/F12新鲜培养基诱导9 d。

If G satisfies max{dG(u,dG}≥ n/2 for each pair of nonadjacent vertices u,v ∈ V, then G has a fractional k-factor.

若对于每一对不相邻的顶点u,v∈V都有max{dG,dG}≥n/2,则G有分数k-因子。

For keeping brittleness,oenanthe Javanica was dipped in 0.2% CaCl2 for 30min.

采用质量分数为0.2%的CaCl2溶液对水芹菜进行浸泡30min可明显改善其脆度。

When the aldehyde content of DAC is 50%, the best absorption capacity of oxycellulose for urea can be achieved.

氧化纤维素醛基质量分数为50%时对尿素氮的吸附性能最高。

The fractional area changein different parasternal crosssectional levels of short axel of left ventricular cavity were studied with manual and acoustic quantificationmethod in 25 subjects.

用手动轨迹球法及声学定量技术对25例二维超声心动图不同左室短轴切面的面积变化分数进行比较研究。

The thickness of the removed surface layer was dependent upon polishing time, current density and the concentration of perchloric acid.

分别在体积分数为5%, 10%和15%的三种高氯酸溶液中和在20, 25和30 Adm^(-2)三个电流密度下进行电化学抛光。

Moreover, with the increase of undercooling, the volume fraction of peritectic phase increases and microstructure refinement occurs.

随着过冷度的增大,包晶相体积分数增大,两相枝晶发生细化。

Aggregate score repositories may be a blight on the pimply countenance of games journalism, but they may also be able to tell us something about the ...

总计分数存放可能成为疫病对pimply国家进行的游戏新闻,但他们可能还可以告诉我们一些有关。。。

To the third quarter intel plans to bring the fraction of polynuclear processors in the assortment of its own production to 80%.

到第三季度英特尔计划把分数多核处理器在自己的生产品种80%。

For example, in the third quarter of 2007 intel expects to bring the fraction of polynuclear processors in its assortment to 80%.

例如2007年第三季度英特尔预计将分数多核处理器在其致病的80%。

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Do you know, i need you to come back

你知道吗,我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。

Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。