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The puerperant and her relatives all signed informed consents. METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity.

无菌条件下截取流产胎儿双侧四肢骨,PBS冲洗髓腔,离心去脂肪及上清液,L-DMEM重悬细胞,沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中,离心后吸取中间界面白膜层,加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基,接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液,去除未贴壁细胞,以后每2 d换液一次。

It was revealed that the film was constituted mainly by Zr-C, C=C (s^2) and C-C(sp^3) bonds; structure of the films was crystalline ZrCN embedded in an amorphous carbon and carbonitride matrix. With increasing flow rate, film Zr content decreased, but C and N contents increased.

实验结果表明薄膜结构是由ZrCN晶粒镶嵌在无定形碳和碳氮化合物基体中;膜中的化合键主要以Zr-C,C=C(sp^2)和C-C(sp^3)形式存在;随着混合气体流量的增加,薄膜的锆原子分数减小,而N和C的原子分数逐渐增大。

In this context, we have reported recently that the volume of myocardial tissue occupied by perimysial and endomysial collagen was lower in hypertensive patients with HF and depressed ejection fraction, than in hypertensive patients with HF and preserved ejection fraction and normotensive subjects.56 Of interest, the interstitial and perivascular accumulation of collagen fibres was abnormally high in the two groups of patients.

在这种情况下,我们已经说了占用体积perimysial心肌组织人肌内膜胶原水平低的高血压患者用高频和沮丧的射血分数在高血压患者的心力衰竭和保存被罚出场分数与正常血压subjects.56舒张压的利息、间质和血管堆积过高的胶原纤维是在两组病人。

For the production of reactive polyurethane cross-linkinger and curing agents,2,4-diisocyanate toluene terpolymer,which possesses the rigid structures of hexatomic ring and three reactive functional groups,was synthesized and characterized by the Fourier transform infrared,the gel permeation chromatography and the chemical analysis methods.

结果表明采用Cat-3作催化剂,在(60±2)℃下反应,可制得分子量为530~550,Mw/Mn=1.10,NCO质量分数为(25.0±0.5)%窄分子量分布的TDI三聚体;随着反应时间延长,TDI会进一步多元聚合生成多聚结构;而加入质量分数为0.4%的苯甲酰氯作为稳定剂,可有效抑制多元聚合的发生,所制备的TDI三聚体稳定性可在半年以上

Isocyanurate was qualitatively identified by infra-red absorption spectroscopy analysis, the quantity of isocyanurate was calculated according to mass fraction of NCO measured by di-n-butylamine method, the thermal stability of prepared elastomers was characterized by thermogravimetric analysis.

用红外光谱法证实了产物中异氰脲酸酯的存在,根据用二正丁胺法测得的NCO基团的质量分数可计算出异氰脲酸酯的质量分数,用热重法分析了产物的热稳定性。

Results The optimum conditions for the extraction of Peucedanum praeruptorum Dunn was as follow: 80 % alcohol was used as solvent, and 8 times the amount of alcohol was added, the mixture was kept refluxing for 1.0 h for the first time; then 6 times the amount of alcohol was added and the mixture was kept refluxing for 0.5 h for the second time; Four times the amount of alcohol for 0.5 h for the last time.

结果 白花前胡的最佳提取工艺为用质量分数80 %的乙醇提取3次,提取时间分别为1.0、0.5、0.5 h,3次所用溶媒量分别为药材质量的8、6、4倍;按照筛选出的最佳工艺条件进行的3次重复性实验,测得浸膏中8-甲氧基补骨脂素含量质量分数为0.094 %,折合1 g生药中含量为0.171 2 mg,与预测值基本一致。

Activity of alkaline phosphatase and ability of formation for calcifying nodules were determined.

抽取健康成人骨髓组织,用Percoll分离液分离出骨髓中的单个核细胞,在含体积分数为10%小牛血清的高糖DMEM培养液中,置于37℃、含体积分数为5%的CO2湿化空气孵箱中培养,通过传代培养扩增骨髓基质干细胞,传三代时改用含地塞米松、β-甘油磷酸和维生素C的条件培养基培养,用倒置显微镜、HE染色观察增殖和分化情况,并测定碱性磷酸酶活性和钙结节形成能力。

Since the scores represent the most precise information they need, they can make accurate decisions based on these scores much faster.

由于分数所代表的最精确的他们所需要的信息,他们可以作出正确的决定,根据这些分数快得多。

Based on the above two experimental studies,we inferred the main combustion characteristic at high altitude which was that the flame radiation coefficient was obviously lower than that in atmospheric region and the mechanism which was due to higher volume fraction of carbon particles than that under atmospheric pressure.To validate this infer,the focus of last experimental study was on the volume fraction of carbon particles in the flame.

前两部分实验研究我们分析得到高原地区的主要燃烧特性是火焰辐射系数明显低于常压地区,其机理是由于火焰中的碳颗粒体积分数高于常压下的,为了验证这一推断,最后一部分实验研究的重点是火焰中碳颗粒的体积分数。

Through research, the relationship among cured depth and concentration of photoinitiator, exposure energy, concentration of monomer, ratio of monomer to cross linker, refractive index of liquid, volume fraction of powders, mean diameter of powders are confirmed.

通过对浆料固化性能的研究,得出了固化厚度随引发剂浓度,曝光能量,单体质量分数,单体和交联剂比例,单体溶液的折射率,粉末体积分数以及粉末粒径的变化关系,并得出相应的最佳值。

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