分支杆菌

Hairy roots were induced from tobacco leaves with Agrobacterium rhizogenes LBA1334 harboring agropin type PRil855 and binary vector PIG121HM carrying a kanamycin resistant gene nptll and GUS gene and the good hairy root clones were selected. The root with many root hairs and branches grew vigorously toward all the direction on MS medium without plant hormone and on kanamyain containing medium (km:30mg/L). The structure of the hairy root tip is different from that of ordinary root. Witch was not typical root cap maybe the main cause of losing the geotropism of hairy root. Plantlet regenerated from hairy root when cultured on Ms medium without plant hormone. The nicotine content of the hairy root was little higher than that in the natural roots.

本研究通过发根农杆菌工程菌LBA1334(含有野生型pRi1855质粒和pIG121HM双元载体质粒，编码NPTⅡ基因和GUS基因)转化烟草沙姆逊，获得了具有卡那霉素抗性烟草发状根，并筛选出良好的单克隆株系，该发状根具有典型的发状根特性(多根毛、多分支、在无激素培养基上快速生长)，通过根尖压片分析，烟草发状根不具备典型的根冠；发状根中烟碱含量1.61％稍高于与天然栽培品种母体根的含量(1.5％左右)，发状根向培养液中释放烟碱，释放量最多时占总烟碱量的81.1％；在无激素的培养基上获得了再生植株。

Meanwhile, a new biger genetic group containing 6 cilicate sWains was formed, and it was different from the reference strains. The phylogenic tree of the representative sWains HLJ01 (GenBank accession EF031269) and HB 16 (GenBank accession EF031270) was constructed by analysis of the 16S rDNA full sequence, the results of analysis of 16S rDNA sequence revealed that swain HLJ01, HB 16 and Bacillus mucilaginosus and Bacillus edaphicus were located in the same phylogenic branch. HLJ01 and HB16 had high homology with Bacillus mucilaginosus in the phylogenic tree, but the 16S rDNA sequence of HLJ01 only has the identity of 96.7% with Bacillus mucilaginosus, and of HB16 has the identity of 95.5% with Bacillus mucilaginosus.

根据16S rDNA-RFLP聚类结果，选取供试硅酸盐细菌菌株HLJ01和HB16作为代表测定了其16S rDNA全序列，其Genbank序列登录号分别为EF031269和EF031270.16SrDNA序列分析表明：代表菌株HLJ01与HB16虽然与胶质芽胞杆菌位于同一个系统发育分支，但相似性分别只有96.7％、95.5％；与土壤芽孢杆菌的相似性则分别为94.6％和93.3％。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失