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In this study, we isolate, purify and characterize endo-β-glucanase by Ascomycete Xylaria regalis. The cellulose-containing medium is used to induce the production of the cellulose-degrading enzymes. When these enzymes are produced at maximum level, we harvest and filtrate the culture medium. The filtrate is purified by anion exchanger column and hydrophobic interaction column (phenyl-650M). The purification fold of endo-β- glucanase increases 4.3 times, the specific activity approaches 3.7 unit/mg, and the recovery is 27.6%, respectively.

为了分离且纯化此株菌产生在培养基中的endo-β-glucanase,先将此菌在cellulose液态培养基中大量培养,在此酵素达到最高峰时,利用滤纸过滤将菌丝及菌液分开,收集粗酵素液,再经过阴离子交换树脂及疏水性层析管柱(Phenyl-650M)等步骤予以纯化,所得到最终酵素纯度为 4.3倍,比活性为3.7 unit/mg,回收率为27.6%。

For things are not really separated, the existence is a whole of asexual climax, and it's an organic entity.

因为事物并不是真正分开的,存在是一个性高潮的整体,它是一个有机的联合体。

Coral polyps can reproduce asexually, forming cloned buds that separate from the "parent."

珊瑚虫可以是无性繁殖,形成无形繁殖的芽再分开来自它的:"双亲"。

If we do not hang together, we shall most assuredly hang separately.

如果我们不能密切的合作,我们就因该最大可能的被分开

Those,whom God has joined together,let no man put asunder .

对那些由上帝结合在一起的人,谁也不要分开

Divided there is little we can do -- for we dare not meet a powerful challenge at odds and split asunder.

分开的,我们没有能做的——在争执或五分四裂的情况下,我们不能面对强大的挑战。

According to Danto, W.H. Auden's poetry "is indistinguishable from ordinary talking."

照丹托看来,奥顿的诗"和寻常的拉呱不可能划分开"。

Auster writes in a little apartment about three blocks from the house he shares with his wife (Hustvedt has "a very nice room" on the top floor of their house where she works:"I think it's better for both of us to be apart during the day.").

奥斯特在离家三个街区的一间小公寓里写作,(而他的妻子Hustvedt则在家里顶楼一间很舒适的房间里工作:"我觉得对我们两人来说,白天分开工作比较好。"

But you are gonna have to get away from your friends.

但是你必须与你的朋友分开

Before going to bed at the hairline from the forehead back comb the scalp at the hairline, hands the little finger connected to the other four fingers naturally separated along the top of the head backward in order, so you can make facial massage to a transparent and eliminate fatigue, promote sleep Gallbladder Meridian .

睡前从前额发际线处向后发际线处梳理头皮,双手小指相连,其余四指自然分开,沿头顶向后梳理,这样可以按摩到使面色通透、消除疲劳、促进睡眠的胆经。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。