分子生物学

These studies cover all areas of molecular, cellular, organistic, population, and ecosystem aspects of marine biology.

对海洋生物学的研究覆盖了分子、细胞、器官、种群和生态系统各方面的所有领域。

The SP cells in the ovarian cell 2780 strain possess the traits of ovarial CSC and expressed ABCG2 molecule maintains the SP cells phenotype,especially in multidrug resistance.

A2780细胞株中的SP细胞基本符合CSC的生物学特征，其高表达的ABCG2分子参与维持SP细胞表型，特别是多药耐药性。

The Emergence of Oxygenic Photosynthesis Plants carry out one of the most difficult chemical reactions in biology: the oxidation of water to protons, molecular oxygen, and, most importantly, high energy electrons for subsequent use in reductive biosynthesis.

产氧光合作用的出现植物执行生物学上最难的化学反应：氧化水产生质子和分子氧，最重要的是，还产生高能量的电子，随后在还原的生物合成反应中利用。

To verify pathopoiesis of PRKAG2 with a novel missense mutation G100S responsible for Chinese PRKAG2 cardiac syndrome and promote pathogenic investigation of the PRKAG2 gene,we study the functional consequences of the mutation in the level of the cell and molecule by overexpressing mutants of PRKAG2 in CCL13 cells.

为了验证PRKAG2 G100S新突变在中国人PRKAG2心脏综合征家系中的致病作用，深入认识PRKAG2 G100S错义突变引起的功能变化，推动PRKAG2基因的致病机制研究，我们通过在CCL13细胞中过表达突变基因，从细胞、分子水平研究了PRKAG2 G100S突变的生物学功能。

To elucidate the molecular mechanisms underlying the neural plasticity after CNS injury, the gene expression profile in the rat hippocampus following perforant path transections was explored by several methods including custom differential screening, custom cDNA array and cDNA microarray. Of 2300 cDNA clones from low-abundance rat brain cDNA bank, 6 were identified as differentially expressed genes/ESTs in the hippocampus 35 days after lesion, in which none was confirmed by Northern blot. Of 8000 cDNA elements, custom cDNA array identified 47 as differentially expressed genes/ESTs with above 3-fold changes in the hippocampus 35 days after lesion, in which 25 were up-regulated and 22 down-regulated.

中文题名去传入海马的差异表达基因筛选和Cystatin C表达副题名外文题名 Screening of the differentially expressed genes and cystatin C expression in the deafferented hippocampus 论文作者应国新导师周长福研究员学科专业神经生物学研究领域\研究方向学位级别博士学位授予单位中国科学院上海生命科学研究院学位授予日期2002 论文页码总数121页关键词海马星型胶质细胞 Cystatin C 基因表达去神经海马馆藏号BSLW /2003 /Q42 /27 为了阐明中枢神经系统损伤后神经可塑性的分子机制，本实验探索了多种方法，包括传统差异筛库、传统cDNA array和cDNA microarray，对穿通纤维切断后大鼠海马的基因表达图谱进行了研究。

One effective way of addressing this issue is to explore the relationship between molecular evolution of functional genes and their phenotypic effects.

基因型变异和表型变异的相互关系问题是尚未解决的生物学基本问题之一，而解决这个问题的一个有效方法是研究基因组中功能相关基因的分子进化和表型效应的相互关系。

This paper reviewed the research advances in the resistance to in-suck fungicide, including the biological characteristics of resistant and sensitive phytopathogenic fungi, the mechanism of in-suck fungicide to phytopathogenic fungi, the resistant mechanism of phytopathogenic fungi to in-suck fungi, molecular detection techniques of fungicide resistance to phytopathogenic fungi and the application of the resistance-fungicide of phytopathogenic fungi.

本文综述了近年来国内外植物病原真菌对内吸性杀菌剂抗药性研究在抗性菌和敏感菌的生物学特征、内吸性杀菌剂对植物病原真菌的作用机制、植物病原真菌对内吸性杀菌剂的抗药机制、抗性菌株的分子检测技术以及抗药性利用等方面的成果。

To study the biologicaleffect of pulsed electromagnetic fieldson the proliferation of Immunomagneticseparated human precartilaginous stem cellsin vitro.To establish immortalizedhuman precartilaginous stem cellsstrain and provided stable cell resource to studythe molecular mechanism of gene targeting on differentiation of PSCs.①The cells from the aborted fetus\'s metaphysis were digested by collagenase,the PSCs were isolated by magnetic-activated cell sorting,then were subculturedand amplified.

探讨体外分离、培养人前软骨干细胞的可行性，分析其表型特点；研究脉冲电磁场对免疫磁性细胞分选的人前软骨干细胞增殖的生物学影响；构建猿肾病毒40大T抗原基因(SV40Tag)介导的永生化人前软骨干细胞株，为下一步基因打靶研究其分化分子机制提供稳定的细胞来源。

To study furderly the activity of CNTF mutant designed by computer molecular modling,this study used the methods of dissociated cultures of chick dorsal root ganglion、TF-1 prolification and the normal mice′weight loss tests.

为了进一步研究我室应用计算机分子模拟设计并表达纯化的睫状神经营养因子突变体蛋白的生物学活性，分别采用鸡胚背根神经节无血清培养法、TF-1细胞增殖法、正常小鼠减重法对其活性进行研究。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

中文题名斜带石斑鱼生长激素分泌及其基因表达调控的研究副题名外文题名 Regulation of growth hormone and its gene expression in orange-spotted grouper，Epinephelus coioides 论文作者冉雪琴导师林浩然学科专业水生生物学研究领域\研究方向学位级别博士学位授予单位中山大学学位授予日期2003 论文页码总数140页关键词生长激素基因表达石斑鱼馆藏号BSLW /2003 /Q959 /39 应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原，免疫家兔制备特异性抗体，通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交／核糖核酸酶保护试验及化学发光检测技术，研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制，对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。