分子生物学

Methods A clinical infection fungus strain was isolaled from mouth pseudomembrane vaginal,nasal secretions,urine,faces,and live puncture tissue,injured skin tissue of a female patient,who had no clear underlying disease.

方法从 1名未确诊女性患者受损皮肤，皮痂，口腔伪膜，阴道及口腔分泌物，尿便及肝穿刺组织，分离与培养出一种真菌，经生化分析，菌种鉴定分子生物学实验及DNA测序。

In this paper,the research progress on psychrophilic methanogens was reviewed,with the focus on thei...

本文针对研究者广泛关注的热点问题，从分离培养及生理生化特性、适冷机制和分子生物学研究几个方面，对嗜冷产甲烷菌的研究进展进行了全面的综述，并对其在低温厌氧生物处理技术中的应用前景进行了分析和展望。

In this paper, the research progress on psychrophilic methanogens was reviewed, with the focus on their culture, isolation, physiological and biochemical characters, molecular biological characters, and cold -adapted mechanisms. The application prospects of psychrophilic methanogens in low-temperature anaerobic technology were also analyzed.

本文针对研究者广泛关注的热点问题，从分离培养及生理生化特性、适冷机制和分子生物学研究几个方面，对嗜冷产甲烷菌的研究进展进行了全而的综述，并对其在低温厌氧生物处理技术中的应用前景进行了分析和展望。

Psychrophilic methanogens ; low-temperature anaerobic bio-treatment ; cold-adapted mechanism ; molecular biological technology

嗜冷产甲烷菌；低温厌氧生物处理；适冷机制；分子生物学技术

Along with progress of molecule biology,gene engineering will be very important approach to quinic acid.

随着分子生物学的不断发展，结合现代生物技术，应用基因工程方法，必将成为获得奎尼酸的重要途径。

Objective To investigate the cause of multiplicity,recurrency and invasiveness of bladder cancer on biological basis.

目的 探讨膀胱癌多发，复发和浸润的分子生物学基础。

Beijing Medical University,Beijing 100034 Objective To investigate the cause of multiplicity,recurrency and invasiveness of bladder cancer on biological basis.

目的 探讨膀胱癌多发，复发和浸润的分子生物学基础。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

This paper mainly investigated the effect of maternal undernutrition during late pregnancy on the levels of hormones which take the somatotropic axis as the center in ewes and lambs, the expression of partil hormones'mRNA and the gene localization in tissue cells on the basis of animal nutrition, endocrine physiology and by modern molecular biology technique.

中文摘要：本研究依据动物营养学和内分泌生理学理论，应用现代分子生物学技术对限制营养的妊娠母羊和羔羊以内分泌生长轴为中心的激素水平变化和部分激素及其受体mRNA表达水平的影响以及在组织细胞中基因表达定位规律等进行研究，进一步探究反刍动物补偿生长的机理，为更科学地将动物的补偿生长能力应用于畜牧业生产上奠定理论基础。

This study mainly investigated the effect of maternal undernutrition during late pregnancy on the levels of hormones which take the somatotropic axis as the center in ewes, the expression of partil hormones'mRNA and the gene localization in tissue cells on the basis of animal nutrition, endocrine physiology and by modern molecular biology technique. The experiments explored the mechanism of restriction and compensatory in order to pave the way for applying compensatory growth for animal husbandry scientifically.

本研究依据内分泌生理学和动物营养学理论，应用现代分子生物学技术系统研究了限饲及补偿生长对母羊生长轴激素基因表达水平的影响以及部分激素在母羊组织细胞中基因表达定位规律，进一步探究了绵羊补偿生长与内分泌生长轴的关系，为更科学地将动物的补偿生长能力应用于畜牧业生产上奠定理论基础。

The basic concept of FOP can be summarized as to further optimize effective prescription according to the standard of curative effects and with the aid of modern science and technology and theories of traditional Chinese medicine.

其基本内涵可概括为：以确有疗效的中药复方为研究对象，以现代科学技术和传统中医药理论为技术支持，以该复方所治病证的药效响应为评价标准，以优化重组疗效更优的新复方为研究目的。

Ever since our world has been a world, native forests have been indiscriminately exploited by man.

自从我们的世界一直是世界原生森林被任意剥削人。