分子生物学

This research, which provides new molecular level data for species identifying and resources evaluation of different geographical Engraulis japonicus populations in Huanghai and Donghai sea areas, fills up the blank in the fields of population genetics and molecular biology of marine animals.

本研究填补了海洋动物种群遗传学与分子生物学领域的空白。

Therefore, the object of this study is to investigate the prevalence of both enteric virus in Taiwan by molecular method (RT-PCR and ELISA).

因此，本研究的目的是以分子生物学的方法(RT-PCR和ELISA)调查轮状病毒与肠病毒在台湾的交叉流行期分布。

Screening toxic proteins to M alternatus from entomogenous fungi is the base of control pest. It will reveal the mechanism how the fungi infect their host from the view of biochemistry and molecular biology, and base for culture trees of anti-longhorn beetle.

本研究是从虫生真菌中寻找对松墨天牛有毒杀作用的蛋白，该研究可从生物化学和分子生物学的角度揭示虫生真菌的致病机理，同时为培育抗天牛苗木奠定基础。

Homology modeling of 3-D structure of two AChE from L.entomophila were constructed using H.sapiens(1p0i:A) native BuChE structure and Drosophila melanogaster(1d×4:A) native AChE structure as templates,respectively,by SWISS-MODEL.The catalytic triad were found and denoted in the 3-D structure of AChE from L. entomophila referring to T.californica.2.2 Gene cloning ofβ-actin and mRNA expression levels of two AChE genes from L. entomophilaBecause no reference gene has ever been used in Real Time PCR for L.entomophila in GeneBank,a fragment ofβ-actin gene was cloned from L.entomophila(GenBank Accession No.: FJ041117).It consists of 822 bp encoding a protein of 273 amino acids residues.

利用蛋白质结构同源建模工具，分别以人丁酰胆碱酯酶(1p0i：A)和果蝇乙酰胆碱酯酶(1d×4：A)的蛋白晶体结构为模板，对嗜虫书虱2个AChE的三维结构进行同源建模，并在三维结构中发现了AChE的酶解活性位点，证明嗜虫书虱体内也存在2个AChE基因。2.2嗜虫书虱β-actin基因克隆及乙酰胆碱酯酶基因mRNA表达水平研究目前关于嗜虫书虱的分子生物学研究较少，在GenBank中没有可用作内参基因的序列，因此本研究从嗜虫书虱体内克隆获得β—actin基因片段(GenBank登录号：FJ041117)，该片段长度为822 bp，编码273个氨基酸残基，同源性比对分析表明该片段与其它昆虫的β—actin基因具有很高的同源性。

Pentylenetetrazol is a central nervous system drug that induce chronic and acute epilepsy model to study epileptogenous mechanism .Kindling is a natural epileptogenous phenomenon.

我们前期研究工作发现：应用电镜、Timm染色等从形态结构方面已证实在点燃前神经元发生了可塑性改变包括苔藓纤维芽生，新突触形成(突触密度增大或数量增多)与突触重排等，但这些只是一个初步探索，仍不能够说明问题的全部，如其分子生物学基础、神经元凋亡情况等尚不清楚，需进一步研究探讨。

In term of molecular biology, we adopt RAMP in the study for indentifltion of Hericium erinaceus, which were widely cultivated in Jilin province .DNA were extracted. PCR expansion and gel. The results show that there were plentiful expression and great specificity on DNA patterns among strains and the degree of specificity is different, too.

在分子生物学方面，本论文采用RAMP技术，对猴头菌属菌株进行DNA提取、PCR扩增及图谱分析，发现各菌株在图谱上均有丰富的表达，并呈现很强的特异性，其差异程度也各不相同。

Its pathologic mechanism has the close relation to high blood sugar, the excrescent of kidney blood dynamics and the household hereditary, etc. Following the improvement of molecular biology, in recent years, many people discovered that many varieties of cell factors take part in the process of DN and exertive important functions.

近年来随着分子生物学技术的进步，研究发现有多种细胞因子参与DN的发生发展过程，其中以转化生长因子、血小板衍化生长因子为核心因子，其作用涉及到肾小球血流动力学改变、细胞外基质代谢、细胞增殖和细胞肥大等诸多方面。

T2 plants of the 2 transgenic plants are analyzed by PCR and positive plants are gained which shows the exogenic gene can inherit generations.

充分利用分子生物学证据证明了花粉管通道法转化大豆的可行性。

With the development of molecular biology, based on yeast two-hybrid, many techniques such as yeast one-hybrid, reverse two-hybrid, three-hybrid, and extranuclear two-hybrid were set up to be applied in the research on the interaction between protein-protein, protein-DNA, protein-RNA, and protein-ligand.

随着分子生物学的发展，在酵母双杂交的基础上又建立起了酵母单杂、反向双杂交、三杂交及核外双杂交等多项技术，并已经成功地运用于蛋白质之间、蛋白质与DNA、RNA和配体之间相互作用的研究。

In particular, it is suggested that resistance mechanisms to environmental stresses and related aspects in molecular biology will be the areas of emphasis in the future, in order to expand Festuca arundinacea research and provide scientific basis for its utilization.

展望了高羊茅在生理生态以及生化特性方面的研究趋势，特别是高羊茅的抗性机理以及有关分子生物学方面的研究将成为未来研究的重点和热点，为今后深入开展高羊茅的研究和利用提供科学依据。

The basic concept of FOP can be summarized as to further optimize effective prescription according to the standard of curative effects and with the aid of modern science and technology and theories of traditional Chinese medicine.

其基本内涵可概括为：以确有疗效的中药复方为研究对象，以现代科学技术和传统中医药理论为技术支持，以该复方所治病证的药效响应为评价标准，以优化重组疗效更优的新复方为研究目的。

Ever since our world has been a world, native forests have been indiscriminately exploited by man.

自从我们的世界一直是世界原生森林被任意剥削人。