分子生物学

Methods:DNA from 358 patients with prostate cancer were assayed for the number of CAG repeats.

采用分子生物学方法对358例前列腺癌患者AR基因CAG进行测试，分析CAG长度与临床各种指标的关系。

In order to overcome the problem of multidrug resistance in human epidemic carcinomata anti-adriamycin cells (KB-A-1), the antisense and antigene oligonucleotides were used to investigate their effectiveness on inhibiting the mdr1 gene expression. The effectiveness of antisense or antigene oligonucleotide on inhibiting the multidrug resistance was detected by MTT colometric assay and ELISA.

中文题名反义与反基因寡核苷酸及其萘二酰亚胺偶联物对靶基因表达的抑制作用研究副题名外文题名 The inhibition of the targeted gene expression by the antisense or antigene oligonucleotides and their naphthylimide-conjugated derivatives 论文作者李军生导师张元兴魏东芝教授学科专业生物化工研究领域\研究方向生物化学与分子生物学学位级别博士学位授予单位华东理工大学学位授予日期2001 论文页码总数106页关键词基因表达基因治疗反义寡核苷酸反基因寡核苷酸核酸馆藏号BSLW /2001 /Q78 /264 针对人表皮癌抗阿霉素细胞株（KB-A-1）的多药抗药性问题，本文从反义核酸和反基因核酸角度，通过MTT法检测细胞生长情况，ELISA法检测基因表达产物P-gp表达水平的变化，对寡核苷酸抑制肿瘤细胞MDR1基因表达的机制进行了探讨。

Acremonium. However, low frequency of integrative genetic transformation is a major obstacle to gene cloning in Cephalosporium acremonium, for example, screening and identification of genes from a DNA bank by complementation of blocked mutants, knock out or replacement of genes.

过去的一些研究表明质粒对顶头孢霉的转化率太低，限制了许多分子生物学技术的应用，如利用互补突变株从顶头孢霉的DNA文库筛选和鉴别基因、基因敲除或者基因替换。

Objective To observe the expression of bone morphogenic protein-2(BMP-2),transforming growth factor-β1(TGF-β1) and vascular endothelial growth factor in fracture healing after treated with Cervus and cucumis polypeptide injection so as to find out its action mechanism.

目的观察鹿瓜多肽注射液对骨折愈合过程中骨形态发生蛋白2(BMP-2)、转化生长因子(TGF-β1)和血管内皮生长因子等生长因子表达的影响，了解鹿瓜多肽注射液促进骨折愈合的分子生物学机制。

Thus, the development of an effective treatment tosolve the chemoresistance of pancreatic cancer remains an urgent task.

近年来随着分子生物学研究的深入发展，以凋亡抑制蛋白为靶点的基因治疗备受关注，目前的实验研究已经取得了初步的成效和进展，可能为有效提高胰腺癌治疗效果提供了一个新的方向。

However takes one kind of precious plants,not actually have the reseach for the chimonanthus praecox in molecular biology.

在分子生物学技术不断取得前进的前提下，人们发现在植物的花药发育和花粉成熟过程中，有大量的基因表达，其中有许多是花药和花粉组织特异性的，目前花药和花粉特异性表达的基因及其调控序列已经成为研究的热点。

Besides these a classification based onthemorphological and molecular biological characters of Chironomus species were carried on in Shenzhen,south of China. All of these studies have laid a basic foundation for the IPM of Chironomus Kiiensis.

并针对深圳地区摇蚊种类的多样性和形态相似性，利用分子生物学方法对摇蚊种类进行了初步分类。

The various parameters observed were the following : modification of the skin status, coat, eyes, mucous membrane, apparition of secretions or excretions and neurovegetative reaction (tears, piloerection, variation of the pupillary diameter, variation of the respiratory rhythm…), walking modifications, changes in the posture, reaction to manipulation, presence of clonic or tonic movements, stereotyped or unusual behaviors, sensorial reactivity to various stimuli (auditory, visual, proprioceptive), prehension strength and moving activity.

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Vascular hyperpermeability is one of the important causein hypovole-mia and organs disfunction after various kinds of shock,and its treatment is still one of the problems in the medical field. For example,the operative methods of preventing hyperpermeability and plasm bleeding is still unreliable after severity burn,and traditional treatment just passively transfuses according to the burn clysis formula instead of inhibiting permeability by injecting drugs. The principal reason is that pathogenesis of vascular endothelial cell permeabilty has not been entirely revealed at the level of molecular biology.

血管通透性增高是休克时有效血容量减少和器官功能损害的重要原因之一，它的防治一直是医学界的一个难题，如在大面积烧伤时，至今未能找到一个简单有效的防治血管通透性升高和血浆渗出丢失的方法，传统的方法只是根据烧伤补液公式测算进行被动的补液来纠正，而不能依靠注射化学制剂来终止其渗出，主要原因是未能从分子生物学水平揭示血管内皮细胞渗出的机制。

So in this paper,firstly the law of somaclonal variation of Lilium concolor,L.regale and L.henryi were investigated systematically from three ways, including Morphology,cytology and molecular biology.Secondly,breeding heat-resistant cultivars for Lilium regale was carried out.

因此本研究从体细胞无性系变异规律入手，选取了耐热性较好的原生种渥丹、岷江百合和湖北百合，从形态学、细胞学和分子生物学三方面进行了较为系统的研究，并在此基础上对岷江百合开展了体细胞无性系耐热选育工作。

The basic concept of FOP can be summarized as to further optimize effective prescription according to the standard of curative effects and with the aid of modern science and technology and theories of traditional Chinese medicine.

其基本内涵可概括为：以确有疗效的中药复方为研究对象，以现代科学技术和传统中医药理论为技术支持，以该复方所治病证的药效响应为评价标准，以优化重组疗效更优的新复方为研究目的。

Ever since our world has been a world, native forests have been indiscriminately exploited by man.

自从我们的世界一直是世界原生森林被任意剥削人。