分子生物学

Besides, motivated by a practical problem from molecular biology we encountered, we have studied the complicated system of inequalities with possible inconsistency involving multilevel "and","or" relationships.

此外，受分子生物学中一个实际问题的促动，我们还研究了包含多层"and"、"or"关系的可能不相容复杂不等式组问题。

They were expressed specifically in pollen, tapetum and ripped anther, showing a high level of S haplotype specific sequence polymorphism, similar to that of the S-RNases.

通过分子生物学方法和杂交授粉试验证明所分离的F-box基因是花粉自交不亲和基因，但目前尚未分离出该类基因相应的表达蛋白。

The recent improvements in the molecular biology and genetics of meningiomas may enhance the abilities to predict prognosis and to develop targe...

近年来，脑膜瘤分子生物学和基因研究的发展在临床提高了对侵袭性脑膜瘤预后的预测能力，并促进发展新的靶向治疗方法以提高疗效。

Since F3, the tetraploidy was maintained from one generation to another and to date FH allotetraploid hybrids were producedF:!

四倍体鱼不但兼有父母本的扩增带，而且也有变异，为四倍体鱼是异源四倍体而不是同源四倍体提供了分子生物学证据。

The cloned BBE gene sequences were 94.84% identified with the reported BBE genes in GenBank previously. Based on the cDNA sequences of COR and BBE ,two fragments about 400～500 bp from each gene with lower identity among them were cloned. The fusion gene BC (744 bp) is fused by the PCR technique. Then the promoter CaMV 35S driven, containing'forward BC fusion fragments-reverse pdk intron-reverse BC fusion fragments', plant siRNA expression vector were constructed based on the vectors pHANNIBAL and pART27.The work will lay the foundation for breeding a low morphine and high thebaine poppy.

利用blast及分子生物学软件DNAStar对COR和BBE基因的cDNA序列同源性进行分析比较，分别从各基因中筛选和克隆了一段同源性极低、约400～500 bp的片段；并应用重叠PCR法将其拼接成744 bp的融合基因BC，以中间载体pHANNIBAL和植物表达载体pART27为基础，构建了以CaMV 35S启动子驱动的含有"正向BC融合片段- pdk内含子-反向BC融合片段"的ihRNAi植物表达载体，通过转化野生罂粟，初步研究了以COR和BBE基因为靶标的RNAi对内源吗啡合成的抑制效果，为进一步培育低吗啡高蒂巴因的罂粟种质提供了依据。

In this dissertation, firstly, the conditions of isolation and purification of specific toxin fractions produced by Exserohilum turcicum has been studied, and a high-efficient method has been established;secondly, the toxins were analysed by UV-Vis spectrophotometry;then the effect of specific toxin on death rate of corn leaves protoplast was studied by FDA as a tinct reagent;finally, using ANS as a probe and SDS-PAGE to study the membrane protein of the corn leaves with Htl gene. In a word, the aim of the research is to search some existent evidences of the toxin binding site on protoplasmic membrane, and provide proofs for nosogenesis of specific toxin in terms of cytology and the molecular biology.

本研究对玉米大斑病菌1号小种毒素的特异性组分进行分离，建立了高效的特异性毒素分离纯化方法，并对其进行了紫外波谱分析；用荧光素双醋酸酯染色法，测定了毒素特异性组分对原生质体死亡率的影响；进而用1-苯胺基-8-萘磺酸荧光探针标记法和SDS-PAGE电泳法对特异性毒素组分与Htl基因玉米细胞互作过程中的质膜蛋白进行了研究，旨在证明Htl基因玉米细胞质膜上存在特异性毒素组分结合蛋白，为从细胞和分子生物学角度阐明毒素的致病机理提供证据。

Mastering microbiology, molecular biology and genetic engineering theory and technology, Plant tissue culture theory and technology, etc.

熟练掌握微生物学、分子生物学及基因工程的理论与技术、植物组织培养的理论与技术等。

In order to clear the aphid and Citrus tristeza disease epidemiology, in this research, to determine the dominant stocks of aphid on citrus by investgatting the occurrence and the damage of the aphids; to clear the relation of aphids and citrus tristeza disease epidemic by analysissing the identity and the efficiency of T.

为了解蚜虫与CTV流行的关系，本试验通过调查柑桔蚜虫的发生与为害，明确柑桔蚜虫的优势种群；就橘蚜传播柑桔衰退病的传毒特性、传毒率、影响传毒率因素等的分析，明确蚜虫在柑桔衰退病流行中的作用；以及对带毒橘蚜体内的CTV分子生物学检测。

Arabidopsis thaliana is always best model plant for the research of the plantmolecular biology, but it is true glycophyte and can unclose less salt-toleranemechanisms.

拟南芥一直是研究植物分子生物学途径的最好模式系统，但拟南芥是真正的甜土植物，因此，使用拟南芥将只能揭示关于植物耐盐性较少的信息。

Molecular techniques are used for uncultured microorganism s to explore the diversity of microbial communities and novel resources without the need of cultivation.

分子生物学技术对不可培养微生物的研究，开启了探索微生物多样性和新资源的大门。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。