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Using semi-quantitative RT-PCR, the differential expression profiles of eleven selected genes were confirmed in the ovaries of triploid and diploid. These genes fell in gene categories with a wide range of functions. The results indicated that triploidy affects the dynamic gene regulatory network in triploid ovary. This study established a firm basis for future investigation on characterization of crucial molecular events for normal ovarian development in shrimp.To further dissect exact gene functions for gonad development of shrimp, three differentially expressed genes between diploid and triploid ovary, PCNA (proliferating cell nuclear antigen), CAS/CSE1 (cellular apoptosis susceptibility protein/chromosome segregation 1) and SSRF (spermatogonial stem-cell renewal factor) were characterized on certain aspects.

利用抑制性消减杂交技术,建立了对虾二倍体和三倍体卵巢间的2个消减文库;在正向消减文库(以三倍体卵巢作为实验组,二倍体卵巢作为驱动组)中,鉴定到54个基因;在反向消减文库(以二倍体卵巢为实验组,三倍体卵巢为驱动组)中,鉴定到16个基因;选取11个差异表达的基因,利用半定量RT-PCR的方法对其在二倍体和三倍体卵巢间的表达进行了检测,均能很好地与消减结果相吻合;这些差异基因编码多种功能的蛋白,分析表明染色体的三倍化使三倍体卵巢中的基因调控网络受到了影响;为深入揭示维持卵巢正常发育的关键分子调控事件奠定了基础。

Besides, it contact with ovarian carcinoma characteristics, clinic classification and tum

Endoglin可能成为在卵巢上皮性肿瘤诊断和生物靶向治疗的重要靶分子。

It is considered that the DNA sequences and amino acid sequences of psaA are conservative, which is unadvisable to use for molecular interspecific classification ofthe genus Phaeocysitis.

因所获得的psaA基因片段序列及氨基酸序列具有种的极端保守性,不适宜用作Phaeocystis属种间的分子分类研究。

Arabidopsis thaliana is always best model plant for the research of the plantmolecular biology, but it is true glycophyte and can unclose less salt-toleranemechanisms.

拟南芥一直是研究植物分子生物学途径的最好模式系统,但拟南芥是真正的甜土植物,因此,使用拟南芥将只能揭示关于植物耐盐性较少的信息。

Fluorescence probe study, surface tension and dye solubilization test indicate the watersoluble calixarene, 5, 11, 17, 23, 29, 35, 41, 47-octosulfonato-49, 50, 51, 52, 53, 54, 55, 56octododecyloxycalix [8] arene (1〓-C〓) acts as"unimolecular"micelle in water.

荧光探针、表面张力及染料增溶实验表明,水溶性杯芳烃5,11,17,23,29,35,41,47-octosulfonato-49,50,51,52,53,54,55,56-octododecyloxycalix[8]arene(1〓-C〓)在水溶液中以单分子胶束的形式存在。

If RB1 molecular genetic testing is not available or is uninformative, indirect testing using polymorphic loci linked to the RB1 gene can be used in familial RB to clarify the genetic status of at-risk family members.

如果无法对 RB1做分子遗传学检测或检测无有效信息,用与 RB1基因连锁的多态位点进行间接的检测同样能用于在家族性 RB 中阐明易感家族成员的遗传状况。

As a result of the evolution of AP equation, parton distribution at small x will lead to the violation of the unitarity of S-matrix.

但AP方程没有包括此效应,因而根据AP方程演化得到的部分子分布在小x区域会导致散射截面幺正性的破坏。

Therefore SFO belongs to a typical circumventricular organs . SFO, Organum vasculum lamina terminalis and area postrema are also called the sensory CVOs by Gross et al, which could sense changes of the bloodbearing molecular signals, so called windows of the brain.

因此它是典型的室周器官(circumventricular organs,CVOs)之一,Gross等人还将穹窿下器、终板血管器和最后区等称为感受性室周器官(sensory CVOs,SCVOs),认为它们可能作为脑的窗口能感受血携免疫信息分子的信息变化。

Molecular genetic studies on HD have been mainly focusing on photoperiod and vernalization, very few studies have involved thermosensory.

植物成花分子遗传学研究主要集中在光周期及春化作用上,关于感温性的遗传研究很少。

However, the yield and quality of cotton have been seriously influenced by the Verticillium wilt. Molecular biology has been employed to make cotton more resistant to this disease.

棉花是我国最重要的经济作物之一,但黄萎病对棉花种植的影响非常严重,使棉花的产量和品质受到了巨大破坏;为解决这个问题,人们尝试利用分子生物学的手段来提高棉花自身的抗病性。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。