分子性

Since most of the large molecules containing phosphorus element have double bonds, it is necessary to consider the special structures of double bonds in order to deal with these large systems more accurately. So, based on density functional theory and electronegativity equalization principle, applying a general atom-bond electronegativity equalization method , using separated program, through regression and least-squares optimization , the parameters of atoms and bonds in molecules are determined . By these parameters , the charge distributions and the energy of some molecules containing phosphorus element, especial the fragment of DNA, RNA, are calculated . The results are quite good.

由于这些含磷生物大分子体系都含有双键，为了明确的处理双键，进而更准确的解释带有双键和共轭双键的含磷生物大分子结构与性质的关系，我们以密度泛函理论~([1-4])和电负性均衡原理~([5-9])为基础，应用和开发原子-键电负性均衡方法中的σπ模型，利用最小二乘法，拟合确定了一些分子（含碳、氮、氧、氢以及磷等原子）的各种类型原子及化学键区域的参数，并利用上述参数计算了一些多聚核苷酸的电荷分布以及分子的总能量，进行了讨论，得到的结果可以和从头算相媲美。

In this paper, suah as Molecular electro-negativity distance vectors , Molecular Electronegativity Interaction Vector were further Studied.

文中对分子电性距离矢量/分子电性相互作用矢量等进行深入的研究。

In chapter five, effect of temperature on the chiral separation of the molecular imprinting polymer was investigated and it was found that the chiral separation process was enthalpy-controlled.

第五章考察了温度对分子烙印聚合物的手性拆分能力的影响，进一步加深了对分子烙印手性固定相分离对映体的作用机理的认识。

Based on these previous work, in this research, in order to explore the the molecular mechanism of enhanced tumor cell immunogenicity treated by elemene and mytomycin C compositely, we used murine Hca-F ascitic hepatic carcinoma ( high lymphatic metastasis substrain of H22) as an experimental model to compare the immunoprotective effects on Hca-F cells as well as the mechanism of the effects caused by HSP70-peptide complex purified from untreated tumor cells, EC-TCV and BCG; We studied the mechanism of increased tumor cell membrane HSP expression treated with elemene by the techniques of RT-PCR and immunoelectron microscopy; In order to deeply elucidate the antitumor effects and the molecular mechanism of elemene in increasing tumor cell immunogenicity we analyzed and compared the effects of elemene and heat shock on the gene expressions of tumor cell using gene expression profile chip.

本论文在上述研究成果的基础上，选择小鼠肝癌H22的高淋巴道转移亚系Hca-F为模型，进一步比较了未处理肿瘤细胞来源、榄香烯复合瘤苗来源的和卡介苗来源的HSP70-肽复合物对Hca-F的免疫保护效应、效应机制，探讨榄香烯-丝裂霉素复合处理增强肿瘤细胞免疫原性的分子机制；以Hca-F和人肝癌细胞株HepG〓等为模型研究了榄香烯处理增强肿瘤细胞膜热休克蛋白表达的机制；以HepG〓为模型用表达谱基因芯片研究比较了榄香烯与热休克处理对肿瘤细胞基因表达的影响，从而进一步阐明榄香烯抗肿瘤作用及增强肿瘤细胞免疫原性的分子机制。

We are interested in the molecular mechanisms underlying neuronal migration and our work published in 1999 indicates that there are diffusible molecules in the brain which can guide the direction of migrating neurons; specifically, a secreted protein called Slit is repulsive to neurons and its concentration gradient guides neuronal migration.

我们在分子水平对这个问题进行了研究。1999年发表的结果给出这样一个答案：脑内存在导向性分子，可以指导神经细胞的迁移方向；具体的发现是，一个叫Slit的分泌性蛋白质，对神经细胞有排斥性作用，它的浓度梯度指导神经细胞迁移的方向。

The results showed that the reaction activity between glycinin (7S subunit) and maltodextrin was high, and the reaction proceeds quantitatively regardless of the variation of protein and polysaccharide mass ratio. The molecular properties of the formed conjugates are also highly uniform. The reaction activity between β-conglycinin (11S subunit) and maltodextrin is relatively lower Compared to that of glycinin, and the molecular properties of these conjugates are affected by the variation of the R values.

电泳分析表明：7S亚基与麦芽糊精的反应性较高，而且在文中所研究的蛋白质与多糖质量比的范围内，7S亚基都能与麦芽糊精发生定量的共聚合反应，形成分子特性高度一致的共聚物；而11S亚基与麦芽糊精间的反应性较低，共聚物的分子特性受蛋白质与多糖质量比的影响。

The effects of active molecule and repressive molecule on the binding process of allosteric enzyme and substrate are discussed by considering the heterotropic effect of the regulating molecule that binds to allosteric enzyme.

文摘：考虑调节剂结合别构酶的异位效应，讨论了与别构部位结合的活性剂分子和抑制剂分子对别构酶和底物结合过程的影响以及它们对协同性效应和抗协同性效应的影响。

Sodium lauroyl sarcosine was used as a denaturant, a novel protein refolding method, which was proposed on the basis of one step-AMC and IEC, one step-AMC-IEC was used on the refolding of denatured/reduced lysozyme.

一步人工分子伴侣-离子交换色谱法辅助还原变性溶菌酶的复性：以月桂酰基肌氨酸钠(Sodium Lauroyl Sarcosine，Sarkosyl，SLS)作为蛋白变性剂，发展了一步人工分子伴侣-离子交换色谱法，对还原变性溶菌酶进行了复性研究。

It has also been discovered that, before complete purification, the synthetic HWTX-Ⅰ, after oxidative treatment, showed wider and more asymmetric peaks than the native HWTX-Ⅰ that was denatured and then renatured under the same experimental conditions, which suggests that there is even greater conformational unhomogeneity in the synthetic HWTX-Ⅰ.

推测除了在合成HWTX-Ⅰ复性过程中有少数分子发生二硫键错配外，还有部分分子在固相合成中发生了消旋作用，需要交替采取多种色谱法才能完全分离纯化复性后的合成多肽产物。

The results show that all the samples perform good foaming capability and emulsifying power, which is changed regularly with molecular weight.

结果表明：室温下，不同相对分子质量的HDP-HPCHS均具有良好的泡沫性和乳化性，并且均随其相对分子质量的改变而呈现规律性变化。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。