分子性

To study the molecular mechanism of Larix somatic embryogenesis, a differentially expressed cDNA library of Larix somatic embryo in the period of maturation was constructed using suppression subtractive hybridization. The cDNA from the cultures at the stage of somatic embryo maturation of embryogenic cell line Y35 of L. leptolepis ×L. principis-rupprechtii was used as the tester and the cDNA from its subcultured callus was used as the driver. Eight hundreds randomly selected positive clones were sequenced, and 468 UniGenes were obtained finally.

为研究落叶松体细胞胚胎发生的分子机理，文章以日本落叶松×华北落叶松杂种无性系胚性细胞系Y35体细胞胚成熟阶段培养物的cDNA为实验组，继代培养阶段胚性愈伤组织的cDNA为对照组，利用抑制性消减杂交技术(Suppression subtractive hybridization， SSH)构建了体细胞胚成熟阶段的差异表达基因文库。

To study the molecular mechanism of Larix somatic embryogenesis, a differentially expressed cDNA library of Larix somatic embryo in the period of maturation was constructed using suppression subtractive hybridization. The cDNA from the cultures at the stage of somatic embryo maturation of embryogenic cell line Y35 of L. leptolepis L. princi-pis-rupprechtii was used as the tester and the cDNA from its subcultured callus was used as the driver. Eight hundreds ran-domly selected positive clones were sequenced, and 468 UniGenes were obtained finally.

为研究落叶松体细胞胚胎发生的分子机理，文章以日本落叶松华北落叶松杂种无性系胚性细胞系Y35体细胞胚成熟阶段培养物的cDNA为实验组，继代培养阶段胚性愈伤组织的cDNA为对照组，利用抑制性消减杂交技术(Suppression subtractive hybridization， SSH)构建了体细胞胚成熟阶段的差异表达基因文库。

To study the molecular mechanism of Larix somatic embryogenesis, a differentially expressed cDNA library of Larix somatic embryo in the period of maturation was constructed using suppression subtractive hybridization. The cDNA from the cultures at the stage of somatic embryo maturation of embryogenic cell line Y35 of L. leptolepis × L . princi-pis-rupprechtii was used as the tester and the cDNA from its subcultured callus was used as the driver. Eight hundreds ran-domly selected positive clones were sequenced, and 468 UniGenes were obtained finally.

为研究落叶松体细胞胚胎发生的分子机理，文章以日本落叶松×华北落叶松杂种无性系胚性细胞系Y35体细胞胚成熟阶段培养物的cDNA为实验组，继代培养阶段胚性愈伤组织的cDNA为对照组，利用抑制性消减杂交技术(Suppression subtractive hybridization， SSH)构建了体细胞胚成熟阶段的差异表达基因文库。

It is especially discussed that the principle, operation process and effects of the operation parameters on the characteristic of the products when the hydrophilic drug micronized with the supercritical assisted atomization which is an improvement on the supercritical anti-solvent technology. It is also introduced that the embedment of hydrophobic drug into biodegradable materials with the use of surfactant as well as the effect of different molecular weight of carrier and different ratio of complex carrier on the characteristic of products is made. It is pointed out that the use of surfactant will improve the micronization of microparticles of the hydrophobic drugs.

在药物微粉化方面，着重介绍了超临界辅助原子化法对水溶性药物的处理，包括该方法的原理、操作方法以及操作参数对产品性能的影响，证明该方法用于改善水溶性药物的微粉化具有良好的效果；在制备药物微球方面，重点介绍了不同操作条件和不同分子质量的载体、不同配比的复合载体对药物微球性能的影响以及表面活性剂在亲脂性及离子型药物微球制备中的应用，指出添加表面活性剂将更有利于得到该类药物的微球。

Sulfonic-4-(β-sulphatoethyl-sulphone) phenylamine is often applied to producing bifunctional-soluble reactive dyes and is an important dye intermediate. It plays an important role in the synthesis of KN style reactive dye M style double reactive dye and modified dissimilar double reactive dye. Used 2-sulfonic-4-(β-sulphatoethyl-sulphone) phenylamine as raw material can improve the uniformly dying performance and scourablility of dyestuff molecules and can improve the firm degree of colour staining.

2-磺酸-4-苯胺是制备水溶性双活性染料的重要中间体，在KN型活性染料、M型双活性染料以及改性异种双活性染料的合成中起着重要的作用，用2-磺酸-4-苯胺作原料合成活性染料可增加染料分子的匀染性和易洗涤性，提高染料的粘色牢度。

This paper reviewed the research advances in the resistance to in-suck fungicide, including the biological characteristics of resistant and sensitive phytopathogenic fungi, the mechanism of in-suck fungicide to phytopathogenic fungi, the resistant mechanism of phytopathogenic fungi to in-suck fungi, molecular detection techniques of fungicide resistance to phytopathogenic fungi and the application of the resistance-fungicide of phytopathogenic fungi.

本文综述了近年来国内外植物病原真菌对内吸性杀菌剂抗药性研究在抗性菌和敏感菌的生物学特征、内吸性杀菌剂对植物病原真菌的作用机制、植物病原真菌对内吸性杀菌剂的抗药机制、抗性菌株的分子检测技术以及抗药性利用等方面的成果。

Finally, the article discussed the possible mechanism of multi-phase electrochemical degradation of wastewater through the XPS spectrum of the used catalysts surface: In the acid solutions, the electro-generated O_2 may get electrons on the porous graphite cathode and then form H_2O_2, H_2O_2 may diffused to the surface of catalyst and reacted with its active part to produce OH, which in turn can destruct organics. The catalyst functioned as both the catalyst to decompose H_2O_2 and the flocculants to sedimentate the degraded waste.

最后全文结合研究反应后催化剂表面的XPS光电子能谱推断了该体系降解污废水可能的反应机理：在酸性条件下，石墨双阳极产生的O_2被多孔扩散性石墨阴极还原成H_2O_2，并通过扩散传质作用在催化剂的表面活性中心分解为强氧化性中间产物·OH，强氧化性攻击有机物，使其降解为小分子有机物或CO_2和H_2O，催化剂在体系中即起催化作用也起吸附、絮凝作用。

NO may be one of the important molecular regulators to permeability of strial capillary endothelial cells by TNF-α,and changes of F-actin content probably relate to the regulation in these cells.

TNF-α能增加耳蜗血管纹微血管内皮细胞的通透性，NO可能是TNF-α调控内皮细胞通透性的重要分子机制之一；耳蜗微血管内皮细胞中F-acfin含量的改变可能与其通透性的调控有关。

This suggested that the purified and dialyzed SjR47 fractional protein should have better immunogenicity and immunoreactivity, and share egg origin antigen.

实验结果表明，经纯化透析后的SjR47组分蛋白具有较高的免疫原性和免疫反应性，该蛋白分子属血吸虫卵源性组分抗原。

Most of water-soluble cyanine dyes have -SO3H and –COOH in their molecules, which will influence the biomolecules' functional characters. A new kind of water-soluble cyanine dyes with several cations in molecules were designed and synthesized, preparation conditions of intermediate and dyes were researched and optimized, the yield of dye is 25%. The novel kind of cyanine dye are water-soluble , spectrum characters was researched , all the dyes were charactered by 1H NMR and TOF-MS, spectrum in different solvent of novel dyes were focused on, one of the kind dyes show higher fluorescence quantum yield than the result reported. Because of several cations in the structure, it is promising when used in DNA assay and other bio-assay field.

大多数水溶性的菁类荧光染料分子结构中都带有-SO3H和-COOH等基团，过多的阴离子的引入容易造成对生物分子的功能属性的影响，本论文设计并合成了分子结构中带有多个正电荷的水溶性的一类新型菁染料，考察并且研究了中间体和染料的合成条件，最终的目标产物产率达到25％，染料通过了质谱和核磁的表征，新型的染料具有良好的水溶性，研究了新染料在多种溶剂的的光谱性质，其中一种染料的在水中的荧光量子产率比文献报道得到了提高，由于富集了正电荷，这类菁染料在DNA标记等生物中有一定应用前景。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。