分子层

Our three groups in SCBB will conduct one project in this grant (three projects in this grant total), which is "Screen mutants interfering the development or regeneration of endodermal organs and study their functional mechanisms". In this project, stem cell and zebrafish will be used as models to elucidate the mechanisms of the development of endodermal organs.

我院的三个课题组负责此项目的课题三《正向遗传突变筛选具有内胚层器官发育或再生缺陷的突变体及致变基因的功能机制研究》，将采用干细胞和斑马鱼模型，研究内胚层器官的机制，特别是分子调节网络的整体性和时空性，为防治肝脏和胰腺的重大疾病提供坚实的理论基础。

METHODS AK was purified by DEAE ion-exchange cellulose(DEAE-52) and sepharose Cl-4B .The component of the saccharide was determined by GC and TLC.Its MW was measure d by gel filtration.The form of glucosidic bond was detrermined by IR.The conten t of the saccharide was measured by colorimetry.

采用DEAE纤维素(DEAE-52)柱层析和凝胶柱层析分离纯化草乌多糖，气相色谱法和薄层层析法测定其多糖组成，凝胶过滤法测定其相对分子质量，红外光谱测定其苷键类型，比色法测定其糖含量。

Through PCR and sequencing, a fragement sequence with lamin gene feature was obtained. It was of of transcriptional activity. In addition, we also cloned and identified giardial DNA topoisomerase gene.Our phylogenetic analysis showed that although Giardia diverged early but not first, thus, it is not as primitive as thought before.

然后进行了核纤层蛋白基因的扩增和鉴定，获得了一个具有明显核纤层蛋白基因特征的基因序列片段，并证明它是表达的且不具内含子；此外还克隆鉴定了作为核骨架重要成分的DNA拓扑异构酶II的基因；在此基础上结合已报导的不同进化地位生物上的数据进行了分子系统学分析，结果显示贾第虫虽然分支较早，但不象过去所认为的那么原始。

With the help of the rich and varied supramolecule, organic substances, inorganic substance, polymer and biological substance and so on, it can make various design of modifying layers on the surface of electrode. It includes multivariate, multilayers, polycomponent, micronize, etc. That will make the detection more sensitive and more widely applied in analytic chemistry.

化学修饰电极可利用丰富多彩的超分子、有机物、无机物、聚合物和生物物质等，在电极表面进行各式各样的修饰层设计，包括多元、多层、多组份、微型化等，已期达到高选择和高灵敏的检测，使化学修饰电极在分析化学中得到广泛应用。

The well type gas nitrogenation oven uses the multi-layered multistage multi-link multi-spot assignment type ammonia input mode, enables the active nitrogen member fast evenly with the work piece surface union, forms the even fast nitrided level, the coordinate high speed spiral air flue, the effective addressing work piece blind hole and the deep hole nitriding craft, nitrides strongly by the microcomputer programmed control infiltrates, the diffusion technology heating curve.

井式气体氮化炉采用多层多段多环多点分配式氨气输入方式，使活性氮气分子能快速均匀地与工件表面结合，形成均匀快速氮化层，配合高速螺旋风道，有效解决工件盲孔和深孔地氮化工艺，由微电脑程序控制氮化强渗、扩散工艺加热曲线。

By tailoring the scale between hydrophilic region and lipophilic region, smectic, columnar and cubic mesophases were observed in compounds I-F and II-F. By changing the length of the lateral chains, a series of new columnar phases and a non-conventional semctic phase were obtained in III-F.

通过调节亲水部分和亲脂部分的比例，在I和II-F中观察到了从近晶层相到柱相再到三维立方相的液晶相序列；在波拉分子III-F中通过改变侧链的长度，获得了一系列柱相及新型层相。

The electroluminescence devices (ITO/NPB/emitting layer /BCP/Mg : Ag) based on 1, 4-bis (4-N, N-methylamino styryl) naphthalene as emitting layer have been designed by the energy band matching principle.

以自制的&D-π-D&对称型有机绿色发光分子1，4-双（4'-N，N-二甲基氨基苯乙烯基）萘为发光层，在结构为ITO/NPB/BMABN/BCP/Mg： Ag的器件中，研究了空穴阻挡层厚度对器件发光性能的影响。

Because the BPE molecule is long enough to eliminate the interlayered antiferromagnetic interaction, B2 become ferrimagnatic based on the faced-shared trimeric cobalt units below 20K.

因为BPE分子够长足以消除无机层间的反铁磁作用，使得化合物B2显现无机层的氧铁磁性。

If the applied voltage is large enough, the liquid crystal molecules in the center of the layer are almost completely untwisted and the polarization of the incident light is not rotated as it passes through the liquid crystal layer.

如果外加电压足够大时，液晶分子在中心层几乎完全untwisted和两极化的入射光是不旋转，因为它通过液晶层。

The structures of the intercalates formed by reaction of four guests with Zn, Al-HTlc were characterized by XRD and IR techniques. And the charge density on the oxygens of each of the carboxylate groups for each of the four anions was investigated utilizing ab initio (HF/6-31G) method by G98w. The orientation of 1,2-,1,3-,1,4-benzenedicarboxylate or benzenecarboxylate anions between the layers was also proposed.

利用XRD和IR测试技术对样品结构进行表征，UV及HPLC对有机酸的反应量进行定量，并采用Gaussian-98软件包中ab initio分子轨道法(HF/6-31G)计算了各有机酸阴离子的分子结构，分析了其结构与插层行为的关系，并理论结合实验给出了各有机酸阴离子在锌铝类水滑石层间可能的空间构型。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。