分子分离

Objective Molecular typing 12 strains of the Salmonella enteritidis isolated from different origins.

内容摘要：肠炎沙门菌；随机扩增多态性DNA；分型摘要：目的对不同来源的12株肠炎沙门菌分离株进行分子分

The unicellular alga Chlamydomonas offers a simple life cycle,easy culture and isolation of series of mutants,established the techniques and tool kit for molecular genetics and genetics analysis.It is now becoming the model organism for studies on photosynthesis in plant,flagellar assembly and function,cell cycle and circadian rhythms,signal transduction,light perception and cell recognition.

单细胞衣藻由于其生活周期简单，培养方法简便，易于分离得到系列的突变体，并已建立了分子遗传学研究技术与遗传分析系统，成为植物光合作用、鞭毛组装与功能、细胞周期及节律、细胞信号传导与光感受、细胞识别等重要生物学过程研究的模式生物体。

The successive χ2 test begins with the determinations of gametic or zygotic selection types, followed by the estimation of recombination fractions between two markers with the Newton-Raphson iteration method.

对取自MAPMAKER软件小鼠F2群体(含333个体)的5个RFLP连锁标记数据作了共显性分子标记偏分离的分析。

Divergences among living genera in the Eublepharidae and the Eastern Gondwanan lineages (Diplodactylidae, Pygopodidae and Carphodactylidae) may be older than those in the Gekkonidae.

这为壁虎类的早期分化与东、西冈瓦纳古陆的分离相关的假说提供了分子证据。

METHODS AK was purified by DEAE ion-exchange cellulose(DEAE-52) and sepharose Cl-4B .The component of the saccharide was determined by GC and TLC.Its MW was measure d by gel filtration.The form of glucosidic bond was detrermined by IR.The conten t of the saccharide was measured by colorimetry.

采用DEAE纤维素(DEAE-52)柱层析和凝胶柱层析分离纯化草乌多糖，气相色谱法和薄层层析法测定其多糖组成，凝胶过滤法测定其相对分子质量，红外光谱测定其苷键类型，比色法测定其糖含量。

HaNPV is the earliest commercial baculovirus insecticide in China and about 100, 000 hectare cotton farmland had been used such insecticide to prevent Heliothis armigera by the end of 1999. Many aspects of HaNPV such as the isolation and identification of different virus strains, bioassay of their toxicity, infectivity and duplication in culture cells, genome sequencing and some functional genes have been researched.

国内已对HaNPV进行了多方面的研究，包括病毒株的分离鉴定、形态特征、病毒生物活性测定、离体细胞感染和复制、测序和功能基因研究，基因工程病毒杀虫剂的构建等；但对其复制侵染与宿主细胞互作的分子机理，目前仍缺乏足够且深入的了解。

As a kind of artificial molecular recognition materials ,the applications of imprinted polymers including in Chromatogram analysis and separate,SolidPhaseExtraction, emulational transducer and so on which were summarized in detail . Analytic technique of iatric ort was introduced briefly.

对药物残留分析技术也作了扼要的介绍，指出了利用分子印迹技术合成磺胺类药印迹聚合物来分离和测定磺胺类药物，对食品工业和制药工业的质量控制有十分重要的意义。

The number of rabies infections increase these years,because there is no universal investigation in China, so it is unsure about the epidemicsituation of rabies in China. It is essential to study the molecular epidemiology ofstreet rabies virus isolated from the nature in order to prevent rabies infection andunderstand different inquilinous bodies of virus in different areas.

中国自2000年以来狂犬病的流行呈逐年上升的趋势，由于中国尚未开展大面积普遍的狂犬病流行病学调查，所以对中国境内狂犬病的流行情况还不明确，为了有效防制狂犬病，非常有必要对自然界分离的狂犬病街毒进行分子流行病学研究，以了解不同地域不同宿主病毒的流行特点。

Now other separatist leaders—especially in oil-rich Aceh and Irian Jaya, which has vast copper and gold mines—argue that "any other area that has been colonized by the Indonesian government has the right to its independence," as an Acehnese rebel commander recently put it.

现在其他一些地方，特别是石油丰富，有大型铜、金矿的地区Aceh和 Irian Jaya，分离分子们争吵着，一位Aceh的将领说&这是另一个被印尼殖民占领的地方，有独立的权利。&

The near isogenic lines, Ps and Pf, were developed by anther, which serves as materials for isolating and cloning Rf3 gene by using of two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) we had improved in this study.

该特异蛋白质点的出现与消失可能与玉米CMS-S的育性恢复有一定关系，对其纯化分离将为Rf3基因的克隆奠定基础和进一步了解玉米质核互作不育表达模式的分子机制。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。