分子分离

Isolated from the marine tube worm cephalodiscus gilchristi in the Indian Ocean, cephalostatin 1 is among the most potent antitumor agents ever tested by the National Cancer Institute.

中文摘要 Cephalostatin 1是从印度洋的海洋寄生虫cephalodiscus gilchristi体内分离得到的十三环吡嗪双甾体，是迄今为止NCI测试过抗肿瘤活性最好的天然产物分子之一。

The study was conducted to construct the cDNA library of Chimonanthus praecox flower and analyze the expression of LTP cDNA.

构建蜡梅花cDNA文库，分析蜡梅脂转移蛋白基因的表达，为探索蜡梅冬季开花分子机理，分离克隆特色基因奠定基础。

In this article,the development of molecular imprinted technology and mechanism of chiral recognition were introduced.

介绍了分子印迹技术及其手性识别机理的最新研究进展，对该技术在色谱分离领域的应用现状、存在的问题及解决方法进行了重点评述，并展望了其今后的发展方向。

The total RNA from brain tissue of substantia nigra of PD rats is extracted at the 1st, 2nd, 4th and 8th week and comparison is made between it and that from normal rats. The total RNA, after RNA formaldehyde degenerated sepharose electrophoresis and separation and Northern transfer, is adsorbed unto colloxylin membrane. Then ECL labeled Selenoprotein P and an unknown gene fragment are used as probes to hybridize with the RNA on the colloxylin membrane. And the hybridized result is obtained with enzyme-linked immunosorbent assay. Finally, analysis of OD is made using ONE-Dscan software.

分别提取1周、2周、1月、2月的帕金森大鼠（各8只）黑质区脑组织总RNA，并取相对应时间的正常大鼠的脑组织总RNA作为对照，经RNA甲醛变性琼脂糖凝胶电泳分离后，通过Northern转移，将其吸附在硝酸纤维素膜上，然后利用ECL标记的Selenoprotein P和一段未知基因为探针，与硝酸纤维素膜上的RNA分子进行杂交，再通过显影取得杂交的结果，应用ONE-Dscan软件进行光密度值分析。

The sequence variation analyses showed that there are 236 variable and 221 parsimony-informative in the aligned 605 nucleotide sites, in addition, the relative frequenencies of four nucleotides were not equal an this fact showed that the base compositions were biased. The results of phylogenetic analyses showed:(1) the phylogenetic relationships of the Cypraeidae taxa was basically in consistent with morphology-based ones;(2)Cypraeovulinae was monophyletic in all trees, whereas Nariinae and Cypraeinae were not;(3)the monophylies of Mauritia, Palmadusta, Erosaria, Monetaria, Staphylaea and Pustularia were strongly supported by molecular data;(4)Meyer's speculation that Chelycypraea came out from Luria were also supported.

结果表明：(1)宝贝科28种动物的分子系统树大部分与形态学分类一致；(2)在系统树上，疹贝与鼻贝亚科的其它分类群的距离相隔较远，提示它们之间有较远的亲缘关系，建议将疹贝属列入宝贝亚科；(3)卵宝贝亚科包括希达贝、脊焦贝、筛目贝、拟枣贝、焦掌贝以及呆足贝等共计15个属，它们在系统树上构成独立的一个大支系，构成单系群；(4)支持Meyer将龟甲贝从禄亚贝总属中分离出来的观点，但龟甲贝和林西那贝之间的系统发生关系及其分类地位还有待于进一步探讨。

Nepalensis wall polysaccharide was prepared with hot water extraction,ethanol precipitation,dialyzation,and lyophilization.the crude polysaccharide was separated by deae-cellulose chromatography and purified by sephadex g-200 chromatography.the inhibit effect of the polysaccharide on lipid peroxidation of mice liver was conducted by tba method.the model of liver-injury induced by carbon tetrachloride（ccl4was used to validate the variety of the realated ast.results onefold ingredient polysaccharide was obtained after isolated and purified.conclusion the ingredient can remarkably inhibit lipid peroxidation of rat liver and significantly reduce activity of ast.

用热水提取，乙醇沉淀提取粗多糖，再经sevag法去除蛋白，透析去除小分子物质，冷冻干燥得楮头红多糖粗品；采用deae-52纤维素柱层析和sephadex g-200凝胶柱层析进行分离纯化；用tba法考察多糖的抗脂质过氧化作用；采用ccl4急性肝损伤模型，测定其血清中ast变化。结论获得一个楮头红多糖组分。tba法表明该组分对小鼠自发性脂质过氧化具有显著的抑制作用；在保肝试验中，给药组能明显抑制ast的升高，对肝损伤有一定的保护作用。

By molecular typing analysis, it was demonstrated that S. senftenberg isolated in 2006 and S. thompson isolated in 2007 belong to two and one clone types respectively, S. enteriditis and S. typhimurium were the two dominant diarrheic Salmonella found in Shanghai.

结果 血清型谱显示本市不同来源的沙门菌型在分布上存在优势交叉，GSS分离菌株中有7个型为国内首次报告；通过分子分型证明本市2006年的山夫登堡和2007年的汤卜逊病例分别属于2个和1个克隆型。

After separation and purification of the expression products by FPLC,the electrophoric patterns of the various eluted peaks are not different with the culture products on SDS-PAGE.

产物经SDS-PAGE、免疫印迹试验与FPLC分离等分析，均表明该工程菌的共表达产物为AchR α-亚基结构域(相对分子质量为13×103)及其与GroES的络合物(23×103)。

Objective Molecular typing 12 strains of the Salmonella enteritidis isolated from different origins.

目的 对不同来源的12株肠炎沙门菌分离株进行分子分型。

Methods XbaI was used to digest the total DNA of Salmonella enteritidis strains isolated from the food poisoning samples.

对食物中毒中分离的肠炎沙门菌用XbaI酶切总DNA，用脉冲场凝胶电泳法进行分子分型，用BioNumerics软件对PFGE分型图谱进行电子化数据分析，绘出聚类分析图。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。