分子分离

Anthocyanidin extract in methanol showed 3 absorption peaks, at 270, 340 and 520 nm, in the UV-vis spectrum, with bathochromic and hypsochromic shift with methanol+AlCl3 and methanol+AlCl3+HCl, respectively; the ratio of the absorbance at 440 nm to that at 520 nm was 0.29. No fluorescence occurred under UV irradiance, and 5 absorption peaks occurred at 270 nm after HPLC. Five molecular ion peaks occurred on MS: 595, 381, 611, 587 and 571, respectively, and monosaccharides in the extract were identified as rhamuose and glucose on HPLC.

一品红花色素的甲醇溶液分别在270、340 和520 nm 处有 3 个吸收峰；在 440 nm 吸光度与可见光最大吸收波长 520 nm 吸光度的比值为0.29；花色素的甲醇溶液中加入AlCl3后发生红移，再加入HCl后发生蓝移；色素溶液在紫外光下无荧光；色素样品经液相色谱分离后在 270 nm检测有 5 个比较明显的吸收峰；质谱中得到 595、611、381、571 和 589 等对应的分子离子峰；花色素酸解液高效液相色谱图谱和鼠李糖、葡萄糖的出峰时间一致。

A F2 population derived from a cross between apetalous line' APT02 'and normal petalled cultivar 'ZS NO.4' was used for molecular markers and mapping of the gene controlling petal-loss trait in Brassica napus.20 pairs of AFLP primers and 170 pairs of SRAP primers were selected between two parents.

在无花瓣品系APT02和正常有花瓣品种中双4号构建的的F2分离群体中，运用AFLP和SRAP两种标记技术对甘蓝型油菜花瓣缺失基因进行分子标记和图谱定位。

Recently, Auricularia auricular polysaccharide is being paied great attention by scientists because of its significant health protection functions.

由于黑木耳多糖具有重大保健价值而备受学术界重视，本文在前人工作的基础上开展了对黑木耳多糖的提取、分离纯化、分子修饰、降血糖活性等方面的研究。

Methods TGGP was purified from Balanophora japonica Makino by n-hexane and ethyl acetate extraction and column chromatography.

用萃取和柱层析方法分离提纯TGGP，用酶联免疫测定法、天然聚丙烯酰胺凝胶电泳、分子排阻高效液相色谱法检测其抑制HIV gp41六螺旋束结构形成的作用，用细胞－细胞融合方法检测TGGP抑制HIV进入靶细胞的活性。

The BAW detector combined with ion exclusion chromatography is used to determine short-carbon-chain organic acids in beehoney and beverages. NA and NAD in foods is also determined by IC-BAW.

本文以离子排斥色谱分离结合BAW检测，对峰蜜和饮料中的低分子有机酸进行了测定；也用离子交换色谱-BAW测定了常用食品中烟酸、烟酸胺的含量，实现质量监控。

The major results obtained are: PCR-RFLP method is suitable for molecular identification of Shihu; 205 compounds were isolated from 10 species of Dendrobium, including 21 new compounds, and 28 compounds isolated from genus Dendrobium for the first time. Each of the extracts and compounds were screened for anticoagulant and antitumour activities separately. The results indicated that the ethyl acetate extract from Dendrobium chrysanthun has significant anticoagulant function; the antitumour function of the 4 bibenzyl components from Dendrobium chrysotoxum is stronger than that of phenanthrene, of which the erianin has significant anti-angiogenic effect both in vivo and in vitro; components with moderate or stronger antitumour activities were found in the extracts of D. thyrsiflorum, D. chrysanthun and D. trigonopus; quality standard for 5 species of Dendrobium was drawn up use active or indicative components as control.

重要研究结果有：经方法学研究，认为PCR-RFLP法适于作为石斛属药材分子标记鉴定方法；对10种石斛进行化学成分分离鉴定，共分得205个化合物，其中新化合物21个，首次从石斛属分得的28个；对提取物和各单体化合物分别作抗凝血、抗肿瘤等活性筛选，结果说明束花石斛乙酸乙酯部位有显著的抗凝血作用，鼓槌石斛的4个联苄类成分抗肿瘤作用强于菲类，联苄类毛兰素在体内外有显著抗血管生成活性，球花石斛、束花石斛及翅梗石斛均筛到中等或较强抗癌活性的成分；选择有活性或指标性成分作对照品，对5种石斛草拟了质量标准。

The courses in this academic program are political theory, foreign languages, applied computational technology, experimental biology, microbiology, molecular biology, genetic engineering, bioseparation engineering, bioreaction engineering, chemical thermodynamics, mass transfer, chemical process simulation, pharmacology, preparation of finished traditional Chinese medicines, pharmaceutical chemistry, GMP in pharmaceutical industry, liquid-to-liquid extraction, pharmaceutical properties measurement and quality control, pharmacy administration, etc.

研修的主要课程有：政治理论课、外语课、数值分析、计算机应用技术、现代生物学与技术实验、现代微生物学、分子生物学、基因工程、生物分离工程、生物反应工程、化工热力学、化工传递过程原理、化工过程模拟、药理学新进展、中成药的制备、药物化学的进展、制药工业的 GMP、液液萃取基础、药品性能检测与质量控制、药业管理学等。

The courses in this academic program are political theory, foreign languages, applied computational technology, experimental biology, microbiology, molecular biology, genetic engineering, bioseparation engineering, bioreaction engineering, chemical thermodynamics, mass transfer, chemical process simulation, pharmacology, preparation of finished traditional Chinese medicines, pharmaceutical chemistry, GMP in pharmaceutical industry, liquid-to-liquid extraction, pharmaceutical properties measurement and quality control, pharmacy administration, etc.

其工程硕士学位授权单位培养从事生物制药、合成制药、中药开发、药剂、药物性能检测与分析的高级工程技术人才。研修的主要课程有：政治理论课、外语课、数值分析、计算机应用技术、现代生物学与技术实验、现代微生物学、分子生物学、基因工程、生物分离工程、生物反应工程、化工热力学、化工传递过程原理、化工过程模拟、药理学新进展、中成药的制备、药物化学的进展、制药工业的GMP、液液萃取基础、药品性能检测与质量控制、药业管理学等。

Finally, two components mixture of caprylic acid and lauryl alcohol was used to attestate the validity of the process model. The model was also used in the simulation of the paroxetine free base and PB, compared with experimental outcome, analyzed the relatively error between experiments and simulations to further check the rationality of the model, which is beneficial to establish a more perfect model in the future.

应用正辛酸-十二烷醇对模型进行验证，并应用此模型模拟计算了帕罗西汀碱和胡椒基丁醚的分离过程，进一步验证了此模型的合理性，分析了产生实验值与模拟值相对误差的原因，为进一步完善刮膜式分子蒸馏过程模型奠定了基础。

This experiment with dilute alkali swelling on collagen extraction and separation, then purified by gel from collagen, and then glutaraldehyde, carbodiimide, has three types of di-isocyanate cross-linking agent on the plastic recombinant protein molecules cross-linked, the final test of infrared spectroscopy analysis of the results of their cross-linking to determine the extent of the effectiveness of cross-linking cross-linking agent to have an initial understanding.

本实验用稀碱溶胀法对胶原蛋白进行提取与分离，接下来用凝胶层析纯化所得胶原蛋白，然后用戊二醛、碳化二亚胺、己二异氰酸酯三种交联剂对胶原蛋白分子进行重组交联，最后通过对红外光谱测试结果的分析，对其交联程度进行判断，对交联剂的交联功效有一个初步的了解。，谢谢。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。