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Methods: NGF was prepared from mouse submaxillary glands by the way of elution with CM 52 column. The molecular weight and purification of NGF were detected by SDS-PAGE polyacrylamide gel electrophoresis.

以小鼠领下腺为原料,採用CM52多次洗脱的力法分离神经生长因子,SDS-PAGE电泳检测NGF的分子品质和纯度,採用鸡胚背根神经节培养方法检测NGF的生物活性。

In order to identified phase Ⅱ conjugates, the aglycones were identifiedafter rat bile was treated with either glucuronidase or sulfatase firstly. Phase Ⅱ conjugates were also separated anddetermined for their molecular ions by LC-MS, at last they were verified by identifying characteristic product ionsor precursor ions by MS-MS.

胆汁经过葡萄糖醛酸酶或硫酸酯酶水解,鉴定其水解产物,从而确定Ⅱ相结合物,再通过LC-MS分离和确定分子离子峰,最后利用MS-MS寻找特征子离子和母离子的方法进行验证。

Experimental results showed that technology of membrane separation promoted the content and yield of tannic acid significantly, which reached 97.3 % and 84.7 % respectively.

分析结果表明,膜分离技术有效地实现了不同分子质量的物料分级和截留,精品单宁酸的含量为97.3 %,收率为84.7 %。

Your aim is to destroy the detachments of the terrorists.

你的目标乃是摧毁恐怖分子的分离。

An investigation on the acquired thermotolerance of recombinant JP2 ligase was done by applying a chaperonin known as TF55 in thermophile on JP2 ligase reaction.

还分离纯化了JP2的分子伴侣-TF55,并将其应用于增加JP2连接酶的热稳定性研究。

According to the fluorescence measurement results from mixed solution, the association constant estimated for thioridazine hydrochloride-BSA binding and the number of the binding site with Scatchard analysis were 1.2×104 L/mol and 0.98, respectively.

将盐酸硫利达嗪与牛血清白蛋白以不同比例混合并在37 ℃下培养,以流动注射透析采样技术从混合液中分离出游离盐酸硫利达嗪,在线氧化为强荧光化合物后测定其浓度;应用Scrathard方程分析所测数据,求得盐酸硫利达嗪和牛血清白蛋白的结合常数为1.2×104 L/mol,结合位点数为0.98;根据热力学参数推断,盐酸硫利达嗪与牛血清白蛋白之间以疏水作用力为主;进一步基于荧光猝灭现象和Fster非辐射能量转移理论,得到盐酸硫利达嗪小分子与牛血清白蛋白之间的能量转移效率( E =0.497)和结合距离( r 0=3.27 nm)。

A quantitative relationship between the separation factor and the bonded-phase molecular topological index for fullerenes is established.

建立了富烯勒的分离因子与键合相分子拓扑指数间的定量关系。

METHODS The structures of compound I, extracted from Clausena lansium Skeels, and synthesized compound III were determined by single crystal X-ray diffraction analysis. The stereo-structures of compound II and IV were also built up through Tripos force field based on crystal structures of compound I and III. RESULTS The molecular formula and molecular weight were found to be C18H17O2N and 279.34 respectively.

方法用单晶X-射线衍射分析法测定了从植物黄皮提取物中分离得到的化合物I和采用合成方法得到的化合物III的晶体结构,并用分子力学计算方法分别模建化合物II和IV的立体结构。

Preliminary study on molecular characterization of 11 isolates of Citrus tristeza virus.

柑桔衰退病毒分离株分子特征初步研究。

This study was conducted to investigate the CP/HinfⅠRFLP groups and molecular characterization of citrus tristeza virus isolates found in wild Chinese citrus plants.

了解中国野生柑橘上携带柑橘衰退病毒分离株的CP/HinfⅠRFLP组群构成和分子特征。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。