分子分离

At present, the understanding of ecology of acidophilic microorganisms is mainly based on the traditional pure culture and isolation techniques. However, the development of molecular biology provides more scientific methods. The combination of pure culture and isolation techniques with molecular biotechnology will provide more effective methods in this field.

目前对嗜酸微生物生态学的理解主要基于传统的纯菌培养分离技术，但是分子生物学技术的发展提供了更科学的研究手段，将纯菌培养分离技术和分子生物学技术相结合是研究嗜酸微生物生态的有效方法。

QTL Mapping and molecular tagging of resistant genes to TuMV and black rot: The QTLs and their genetic effects of the genes resistant to TuMV and Black rot were mapped and dissected on the basis of the radish molecular genetic linkage map and with the multi-QTL model. It was found that 4 target QTLs intensively locating in LG3 and LG5 linkage groups.

萝卜分子遗传图谱的构建：利用同时对萝卜TuMV和黑腐病存在明显抗性差异的自交系构建了包括360个单株的F2分离群体，以此群体为研究对象，首次采用优化的萝卜SRAP分子标记技术和SSR分子标记技术相结合的方法，构建萝卜分子遗传图谱，该图谱包括9个连锁群，由196个标记组成，图谱总长度736.2 cM，平均图距3.76 cM.4。

They shared the highest homology to "world-B" group isolates in Brassica pathotype (between 91.8% and 100%). Phylogenetic analysis based on the CP gene sequences of TuMV strains or isolates showed that these 6 TuMV isolates from Shandong belonged to "world-B" group.

所得6个分离物的CP基因的核苷酸序列同源性较高，在97.0%～99.4%之间，系统进化分析表明：它们与Brassica致病型分离物中的world-B组分离物的同源性最高（91.8%～100%），亲缘关系最近，从而从分子水平上将TuMV山东分离物划分为world-B组。

Results and Conclusions Molecularly imprinted membrane is the molecular imprinting technique combined with membrane separation technology.

结果与结论分子印迹膜是将分子印迹技术与膜分离技术相结合，近年来被广泛运用于中药有效成分的分离。

The results showed that the two compounds could be separated by the nicotinamide-MIP monolithic column. The column is very useful for the enrichment and detection of nicotinamide in body fluids.

结果表明，原位聚合法制备的整体分子印迹聚合物在以纯水作流动相时对模板分子与其类似物有快速分离能力，这对于体内药物的分离富集研究具有很好的应用前景。

Firstly, the SSH was used to isolate tissues or organs specific expression genes in different development stages of plant. These genes provide evidence for explaining the molecular mechanism of plant development. Then SSH was widely used to isolate genes related to resistance under biological or abiological stress in different plants and the molecular mechanism of the resistance was primarily elucidated.

首先， 官中的组织特异性表达的基因，为揭示植物生长发育过程的分子机理提供了有效手段；另外，在不同植物中，该技术被大量应用于分离各种生物及非生物胁迫条件下诱导表达的抗性相关基因，可以揭示植物抗逆的分子 SSH 技术已开始应用于分离与次生代谢产物合成相关的基因。

Results Crystallin could be well separated from 150 ig of total protein sample using 13% SDS-PAGE. High molecular weight proteins could be well separated from 1.2 mg sample using 10% SDS-PAGE.

结果 当上样量为150 μg，13%的分离胶对晶状休蛋白的分离效果较好，当上样量为1.2mg，10%的分离胶对高相对分子品质蛋白的分离效果较好。

Three infectious bronchitis virus isolates named as Q1, J2 and T3 were isolated from proventricular tissue taken from vaccinated chicken flocks in China The biological characteristics of the three isolates were observed.

中文题名传染性支气管炎致腺胃病变毒株变异的分子基础及我国和东南亚分离株的分子流行病学研究副题名外文题名 Molecular basis for variation of proventriculus pathogenic IBV isolates and molecular epidemiology of IBV isolates from China and southeast Asia 论文作者蒋贻海导师陈溥言教授学科专业预防兽医学研究领域\研究方向动物分子病毒学及免疫学学位级别博士学位授予单位南京农业大学学位授予日期2002 论文页码总数127页关键词鸡传染性支气管炎传染性支气管炎病毒腺胃毒株馆藏号BSLW /2003 /S855 /9 从我国接种过传染性支气管炎疫苗的幼鸡群的腺胃组织中分离得到三个传染性支气管炎毒株Q1、J2和T3，并对它们的部分生物学特性进行研究。

C An attempt is initiated to separate a mixture of different forms of the same molecule and isolate a desired form of the molecule for pharmacological or other reasons (e.g., separation of racemic mixtures).

尝试分离相同分子的不同形态的混合物，并隔离出药用分子的想要获得的形态。或者其它原因（如，消悬体混合物的分离）。

Two molecular forms of Xenopus hatching enzyme, 60kD and 40kD molecules, was obtained during preparation and purification. Both of them were verified as the hatching enzyme molecules, using anti GST UVS.2 antibody as the probe. 60kD molecule was digested or autodigested easily into 40kD molecule during purification. It was indicated that 40kD molecule was probably derived from 60kD molecule with its two CUB repeats lost, and the two CUB repeats may play an important role in recognizing and/or modifying of the molecular structure of vitelline envelope.

在分离纯化非洲爪蟾孵化酶时，得到了60kD和40kD两种分子，用孵化酶的特异性抗GST-UVS.2抗体进行Western杂交的结果证明二者均为孵化酶分子。60kD分子很不稳定，在纯化过程中极易降解，40kD分子可能是由60kD分子经过降解或自身降解丢失了两个CUB重复区而形成的，而CUB重复区很可能在对受精膜分子结构的修饰或改造中具有重要作用。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。