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分子分离

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Conventional forest breeding is constrained by its long reproductive cycle, which includes long juvenile period, and by its complex reproductive characteristics, such as self-incompatibility and a high degree of heterozygosis. Gene transformation has played an important role in woody plant improvement, which can shorten breeding period and breed varieties with high salt tolerance.

随着分子生物学技术的发展,从盐生植物中分离出抗盐基因,导入林木,获得抗盐品种;况且林木由于其较长的幼年期,常规育种有明显的局限性,而通过转基因可以在较短时间内获得高抗盐品种,缩短育种周期,因此开展林木抗盐育种具有重要的意义。

Aim To optimize purification conditions of recombinant hirudin 3 in the fermentation broth and characterize the product.

目的优化分离纯化大肠杆菌发酵液中重组水蛭素Ⅲ的工艺并对所纯化产物进行分子鉴定。

Bacterial strains were isolated from humus mud by employing the dilution methods. Seven cellulose-degradation Strains marked as B-37, B-35,B-31, B-25, B-17, Z-a, and Z-b were selected according to the hyaloid rings on the medium of carboxymethylcellulose sodium Congo red and their efficacy of decomposing filter paper. Z-b had the strongest ability in decomposing filter paper among the seven strains and 16S rDNA analyzed indecated that it was Stenotroplaomnonas maltophilia owning to 99.8% homology. Comparing clony size of the seven strains in media with different carbohydrates and inorganic salt, we found that the potato extract and inorganic salt which contained CaCl2 0.20, MgSO4 1.25, NaCl5.00,(NH4) SO4 1.30, KH2 PO4 1.35, FeSO47H2O 0.015, Na-EDTA 0.02 were suitable for the bacteria growth.

用稀释法从腐殖泥中分离菌株,根据它们在梭甲基纤维素钠-刚果红培养基上的透明圈直径及对滤纸的崩解能力,获得7株纤维素分解细菌(编号:B-37、B-35、B-31、B-25、B-17、Z-a和Z-b),其中菌株Z-b的纤维素崩解能力最强,分子鉴定结果表明它与Stenotrophomonas maltophilia的16S rDNA序列有99.8%的同源性,初步确定为嗜麦芽窄食单胞菌。

Objective: TO explore the molecular mechanism of the components of milkvitch-red sage compound elevating placenta blood supply from the point of view of invading by nutrient cells languishment, formation of placental decidual vas net, endurance mechanism and languishment of nutrient cells.

目的:从滋养细胞侵入、胎盘蜕膜血管网络形成、妊娠免疫监视/耐受机制、滋养细胞凋亡等角度,研究探讨黄芪、丹参复方成分提高胎盘血供的分子机制;方法:分离提取黄芪,丹参有效组分(黄芪总皂甙、总黄酮;丹参提取丹参酮、丹参醌、丹参酸三部分)。

Molecular phylogenetic tree supported that Viverrine was polyphyletic, and implied spotted linsang perhaps was basal and mostly specified to other Viverridae species in China.

使用邻接法、最大似然法重建的分子系统树显示:斑林狸从灵猫亚科中分离出来,支持灵猫亚科的多系起源,而且斑林狸可能是中国起源最早且最特化的灵猫科动物。

At present we have the deeper acknowledge for the molecular mechanism of flowering by studying the long-day plant Arabidopsis which is a model plant for the study of photoperiodic control of floral initiation.

近年来通过对模式植物拟南芥突变体的遗传研究、基因分离及功能分析,使人们对光周期诱导开花的分子机制有了新的认识。

The energy required to separate particles from a molecule or atom or nucleus; equals the mass defect.

为从一个分子或原子或核子中分离颗粒所必须的能量;等同于质量亏损。

The analysis method uses a simplified version of a mass spectrometer, a tool that can be used to separate and identify molecules in compounds.

这种方法运用简化的质谱仪来分析样本。质谱仪可用于分离和识别化合物中的分子。

Results 1555 mutation in the mitochondrial DNA was reverified to be one of the major factors which cause maternally inherited nonsyndromic deafness and the cosegregation of 955-960 and 1555 was present in this family.

结果再次证明了1555突变是该家系成员致聋的分子生物学基础之一;并发现该家系27名母系成员的线粒体基因组中除1555突变外,还同时存在有955-960同质型突变,两突变共分离。

And a glycochain IbGpl-OL-LbGp5-OL has been found out by the way of Pronase E or β elimination reaction to dissociate the glycochain from the molecules of glycoconjugate and through the separation and purification of it by gel column chromatography, and ion exchange ohromatography and then its repetitive unit construction has been tested by the ways and means of full hybridization and partial acid hydrolisis, methylate analysis, Gc, Gc/Ms, iH and 1/3 CNMR.

通过链霉蛋白酶E或β-消去反应等方法,将糖链从糖缀合物分子上解离下来,经凝胶柱层析或离子交换柱层析进行分离、纯化得到相应的糖链LbGp1-OL~LbGp5-OL。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。