分子分离

Truncation mutations in the adenomatous polyposis coli protein are responsible for familial and sporadic colonic tumours. Except for its function as a role in regulating b-catenin, recent studies show that APC has additional roles in cytoskeletal regulation. It binds to microactin and microtubules directly and indirectly. Furthermore, APC plays an important role in mediating the detraction of chromosome.

编码APC蛋白(adenomatous polyposis coli， APC)基因的缺失突变会导致家族性和散发性的结肠癌，APC蛋白除了能直接参与Wnt信号途径调节b-catenin的浓度之外，最近的研究表明APC蛋白能够与细胞骨架的主要成分微管和微丝直接或间接结合，通过调节微管的解聚和聚合，间接调节染色体的分离，作为潜在的细胞骨架调节分子将细胞骨架与重要的细胞信号转导通路紧密联系在一起。

Molecular distillation as a new and high efficiency separation technology has been widely used in many fields.

分子蒸馏技术是一种新型、高效的分离技术，现已在许多领域得到广泛应用。

Molecular distillation technology is a new separate method and has been widely used in light industry.

分子蒸馏技术作为一种新兴的分离技术，在轻工行业得到越来越广泛的应用。

Lyase An enzyme that catalyzes the separation of two parts of a molecule with the formation of a double bond in one of them.

裂解酶：一类能催化一个分子的两部分分离，并在其中一个上形成双键的酶。

It was found that the chiral separation process was enthalpy controlled.

结果表明，分子烙印手性固定相的分离过程为焓控制过程。

Based on the previous literatures, the following major innovation works were carried out in this dissertation:(1) The derivatization reaction conditions of ephedrine and pseudoephedrine with sensitive derivation reagent in aqueous system were studied and two new methods were developed for the assay of them by microemulsion electrokinetic chromatography and micellar electrokinetic chromatography with LIF detection. The sensitivity and analysis times were greatly improved compared with the previous reports;(2) A new method of CZE with indirect LIF detection was developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside) with fluorescein as the probe. The proposed method enlarges the application range of LIF detector, and provides new approach for the analysis of certain compounds difficult to derivatize;(3) Based on the above research, an MEKC with indirect LIF detection method for the simultaneous determination of adenine and guanine in DNA extracts from fungus, maize and soybean was established;(4) A new method for the investigation of the complexes formed between human serum albumin and ampicillin sodium under the simulated physiology conditions using laser light scattering technique was developed.

该论文在综述前人工作的基础上，开展了如下未见文献报道的创新性的研究工作：(1)使用灵敏的衍生试剂对麻黄碱和伪麻黄碱在水体系中的衍生反应条件进行了系统研究，建立了微乳电动色谱-激光诱导荧光检测法和胶束电动色谱—激光诱导荧光检测法测定麻黄碱和伪麻黄碱的灵敏分析新方法，与以前的报道相比，灵敏度和分析时间均有很大改善；(2)使用荧光素钠作为背景荧光试剂，建立了同时分析测定六种黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的毛细管区带电泳—间接激光诱导荧光检测新方法，扩大了激光诱导荧光检测的应用范围，对难衍生化合物的分离分析提供了一种新思路和新途径；(3)以荧光素钠作为背景荧光试剂，建立了一种用于同时测定食品提取DNA中的腺嘌呤和鸟嘌呤含量的胶束电动色谱—间接激光诱导荧光检测新方法；(4)用动态和静态激光散射法研究了模拟生理条件下人血清白蛋白与氨比西林钠盐相互作用所形成的复合物，为药物分子与蛋白质的相互作用、药代动力学等的研究提供了一种新思路。

This dissertation aims to: improvement in regularity of ladderlike polysiloxane through strong H-bonding template and"half-water-hydrolysis"methods; synthesis of several functional polysilsequioxane with ethoxyl, hydro and aminopropyl groups respectively; and the preparation of nanoporous film with good mechanic properties from ladderlike functional polysilsesquioxane with intermolecular porogen via micro-phase separation process.

本论文旨在利用强氢键模板作用和"半水水解-脱氯化氢缩聚"的改进方法，提高反应性的梯形聚硅氧烷的规整度；合成乙氧基封端、含氢基和氨丙基的几种功能性梯形聚硅氧烷；以及探索利用含分子内成孔剂的梯形聚倍半硅氧烷通过微相分离制备低介电常数二氧化硅纳米微孔膜。

A model for computing the electric field dependent rate constants of gas-phase intramolecular electron transfer reactions by classical path method has been proposed within the activated-complex framework.

用经典轨迹方法，在过渡态理论框架下，提出外电场中气相分子内电子转移反应的速率常数计算模型，并用电子定域初始猜测诱导UHF-SCF技巧实现了两电子定域准透热态的分离。

The beheviour of four low-molecule weight organic acid lactic acid,hydroxy-acetic acid,mercapto-acetic acid,glyconic acid as iron-containing silicate-aegirines depressants on the hematite direct flotation is investigated in this paper.The results of flota

本文研究了四种小分子有机酸抑制剂乳酸、羟基乙酸、巯基乙酸、葡糖酸在赤铁矿正浮选中对含铁硅酸盐脉石霓石的抑制行为，纯矿物、人工混合矿与实际矿石浮选实验均证明了它们具有良好的选择性抑制作用，可以成功地实现赤铁矿与霓石的浮选分离。

Which provide molecular evidence that supports the hypotheses that the early cladogenesis of the Gekkota was associated with the split of the Eastern Gondwanaland from Western Gondwanaland.

这为壁虎类的早期分化与东、西冈瓦纳古陆的分离相关的假说提供了分子证据。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。