分子分离

Significant changes encompass strengthening of the coverage of data handling, together with new material covering rapidly developing subject areas of molecular spectrometry, atomic spectrometry, and separation techniques.

显著的变化围绕数据处理的新闻报导的加强，以及包括迅速发展的分子测谱学，原子测谱学和分离技术的学科领域的新材料。分析化学の原則と実践は20年

In this study, AFLP markers for YrSp gene were identified and SCAR markers were developed using the near-isogenic line YrSp/6 x Avocet S and its susceptible parent Avocet S as materials with the YrSp gene donor parent Spaldings Prolific as control. The main results were given as follows:1. A total of 225 primer combinations were used in AFLP analysis of the materials YrSp/6×Avocet S, Avocet S and Spaldings Prolific.

本研究采用AFLP分析方法，以小麦抗条锈病基因YrSp的供体亲本Spaldings Prolific、近等基因系YrSp/6 x Avocet S、轮回亲本Avocet S及由Avocet S和YrSp/6 x Avocet S杂交制备的F_2代分离群体为材料，开展了小麦抗条锈病基因YrSp的分子标记研究，取得了如下主要结果： 1。

The characters and applications of vacuum film evaporation and molecular evaporation are outlined, and an XZN inner-cooling vacuum agitated film evaporator was used to reduce free monomer from HDI biuret , which is for improving the quality of polyurethane paints and coatings, decreasing pollution and danger to worker.

本文介绍了真空薄膜蒸发和分子蒸发的原理、特点和应用，并介绍了真空薄膜蒸发-冷凝工艺在分离HDI 缩二脲中游离单体中的应用。

Methods Models of severe thoracic trauma were established,alveolar macrophages were obtained by collecting broncho alveolar lavage fluid,and then separated and cultured.TLR4 mRNA and the expression of TLR4 protein were measured by northern and western blot before and 2,4,8,16,24h after trauma.

建立大鼠严重胸部创伤模型，支气管肺泡灌洗分离、培养肺泡巨噬细胞，利用Western、Northern分子生物学技术检测创伤前、创伤后2、4、8、16、24小时肺泡巨噬细胞TLR4的表达水平。

A process for preparing cinnamaldehyde from cassia oil with using molecular distillation was investigated.

利用分子蒸馏技术对肉桂油分离提纯肉桂醛工艺技术条件进行了研究。

A technique that uses a centrifugation to separate molecules according to density.

应用离心根据密度对分子进行分离的技术。

The gene of ESAT-6 from genome of bovine mycobacterium tuberculosis strain that from cervine tuberculosis was amplified by polymerase chain reaction and inserted into pGEM-TE vector. After sequencing, we analyzed the gene encoding ESAT-6 protein with molecular biologic software.

从患结核病鹿的肺组织中粗分离牛分支杆菌制备DNA模板，用PCR对牛分支杆菌ESAT-6基因进行了扩增，将扩增的产物连接于测序载体pGEM-TE上克隆，经测序确定无误；并使用分子生物学软件对ESAT-6基因进行了分析。

To study and know well the rudimentary knowledge and the basic techniques of modern Microbiology are demanded for Graduate students. The experiments including the principle and operation of phase contrast microscope and fluorescence microscope; Microphotography , the technique of photographic enlargement;Applications of polymerase chain reaction ; Construction, transformation and detection of plasmid in E.coli ; Infectivity of virus and its molecular detection ; The technique of protoplast fusion in filamentary fungi ; Induction and purification of β-galactosidase from E.coli and assay the enzyme activity; Detection of GC content and DNA-DNA hybridization in bacteria ; Production , extraction and detection of poly--hyduoxy butyrate from bacteria.

以实验教学为主，要求研究生学习和掌握一些现代微生物学研究所需的基本技术，内容包括：相差显微镜、荧光显微镜的使用和显微摄影技术，PCR技术的应用，细菌质粒的构建、转化及检测技术，病毒的侵染性及其分子检测技术，丝状真菌原生质体融合技术，大肠杆菌β-半乳糖苷酶的分离及纯化及其动力学测定技术，细菌G+C mol%的测定及细菌DNA-DNA杂交技术和聚羟基丁酸产生菌的发酵、产物提取及检测技术等。

Capsici has been broadened, there is considerable diversity in host-specific pathogenicity. The isolates of P. capsici in Taiwan belonged to A1 mating type, and no chlamydospore was found. The structure or survival of P. capsici in the field is unkown.

目前在台湾所分离到之茄科疫病菌菌株均为A1配对型，且尚未发现其具有厚膜孢子，故在自然界中此病原菌是以何种构造或方式残存仍属未知，故分子标记之建立可协助该菌在自然界中之生态研究。

The drying process of chrome liquor was studied. The structure and property of chrome complex reducing by alcohol were analysed and tokened by many present well-rounded separation and analysis methods such as IR, UV-Vis Spectrum, IEC and pH potentiometry. Application tests of chrome tanning agents were conducted and tanning methods of tanning at high pH were studied. A systematic study was conducted on high alkalinity chrome tanning agents application in leather making process and possibility of clean chrome tanning was discussed.

本文系统地研究了多种小分子醇与重铬酸盐之间的氧化-还原反应，对反应过程进行了认真的剖析；对铬鞣液的干燥过程进行了研究；用红外光谱法、紫外及可见分光光度法、离子色谱法以及pH电位滴定法等多种比较成熟的现代分离分析方法相结合，对醇还原铬配合物的结构与性能进行了分析与表征；对铬鞣剂的鞣革性能进行了应用实验，研究了醇铬鞣剂在高pH值下的鞣制方法，并就高碱度铬鞣剂在制革过程中的应用进行了系统研究，探讨了用其实施清洁化铬鞣的可能性。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。