分子分离

Methods:(1) Dissoluble PGN and CpG DNA were immobilized onto the surface of biotin cuvette for establishing target. Another effective tracking approach was established by immobilizing Escherichia lipid A F583 onto the surface of Non-derivatised cuvett. The biosensor technology was applied to screen anti-inflammatory TCM targeting on three key molecules.(2) The active compositions were isolated by AB-8 macroreticular resin from lycium bark. After the activities of compositions were evaluated, the most effective compositions was confirmed. In vitro, the affinities of different concentrations composition E binding with PGN, CpG DNA and lipid A were measured separately. The effect of composition E on vigor of RAW264.7 cells were tested by MTT and CCK-8, and its inhibition on TNF-α, which was released from RAW264.7 cells induced by PGN, CpG DNA and LPS, was also tested by ELISA. In vivo, murine sepsis models were made by intravenously heat-killed E.coli and heat-killed S.aureus, then protection of composition E on mice sepsis model were observed.

(1)将PGN及CpG DNA包被于生物素样品池，将lipid A包被于非衍生样品池，分别建立以PGN、CpG DNA及lipid A为靶点的技术平台，对114种抗炎中药水提物进行筛选、评价其活性物质含量，并评估出针对上述三种病原分子均具有较高结合活性的中药；(2)利用生物传感器跟踪检测技术、大孔吸附树脂分离技术，从地骨皮中定向分离与PGN、CpG DNA及lipid A均具有较高亲和力的活性组分；在体外实验中，测定不同浓度活性组分与PGN、CpG DNA及lipid A亲和力；MTT法及CCK-8法检测活性组分对RAW264.7细胞活力的影响；ELISA法检测活性组分对PGN(2μg/ml)、CpG DNA(10μg/ml)及LPS(100ng/ml)刺激小鼠RAW264.7细胞分泌TNF-α的抑制作用；在体内实验中，采用尾静脉注射致死剂量热灭活大肠杆菌和热灭活金黄色葡萄球菌，建立细菌脓毒症小鼠模型，观察活性组分对脓毒症模型小鼠的保护作用。

As endogenous jasmonates exist in enantiomers in nature and at level of nanogram/per gram fresh weight, it is very challenging work to develop rapid and accurate method for qualitative and quantitative analyses of JAs. This review paper summarizes the recent advances in the analysis of jasmonates including chromatography, capillary electrophoresis, chromatography-mass spectrometry and immunoassay.

内源茉莉酸类激素通常以多种手性异构体存在，且含量超微，因此，对内源性茉莉酸类激素进行准确的定性、定量分析具有很大的难度，研究高效分离富集、高灵敏度检测以及分离与检测联用等分析方法，对加速植物激素分子作用机理研究具有重大意义。

The replacement of toxic and volatile organic solvents as reaction media or an efficient catalyst with environmentally acceptable alternatives are an area of tremendous importance in modern organic synthesis. Basing the review about solid-phase synthesis with simple separation, the liquid phase organic synthesis supported by the solution polyethylene glycol and the chemical reactions in the medium of PEG, herein we are going to study the synthesis of 1-amidoalkyl-2 -naphthols in presence of the solution catalyst or the solid catalyst and the synthesis of thiourea in PEG-400 medium.

难溶性载体试剂参与下的固相合成使分离操作简单易行；可溶性聚乙二醇支载下的液相合成显示出液相反应的稳定高效性，同时兼顾大分子聚合物容易沉降分离的优点；无毒且稳定的小分子PEG作为反应介质，往往表现出醚和醇的双重性质和功能。

Chapter Three. The Studies on Gene Therapy for Retinal Degeneration of the Mice and It's Mechanism Objective: To evaluate the abilities of adeno-associated virtus carrying the basic fibroblast growth factor gene as a therapy for retinal photoreceptors in slow retinal degerneration mice on the basis of the experiment results as mentioned above. Methods: On the born day of rds mice, IPE cells were isolated with enzyme-assisted microdissection from pigs and were cultured.

第三章 视网膜色素变性小鼠的基因治疗及其机制目的：在体外实验证明AAV载体可以高效安全转染IPE细胞并且可以表达具有生物活性的bFGF的基础上，应用AAV载体携带bFGF基因以先体外，后体内法和体内法两种方式进行视网膜色素变性鼠(retinal degemeration slow，rds)的基因治疗，在分子生物学、形态学和功能学上进行基因治疗的初步观察方法：在rds鼠出生的当天取眼球，采用酶辅助的显微分离方法分离培养IPE细胞。

The three isolates have four specific replacements in 87 , 88 , 95 , and 119 in S1 hypervariable region, and a unique cleavage site R-X-R-T-G-R in 3' terminal region. These data may be valuable for investigation of molecular basis of the serotyping, tissue tropism and pathogenicity of these virus isolates.

本文的3个分离毒株Q1、J2和T3在S1高变区的87，88，95和119有4个特有的替换，在3'末端有独特的裂解位点R-X5-R-T-G-R，这些结果可能对这些分离毒株的血清型特异序列和组织趋向性和致病性的分子决定区方面的研究有重要的价值。

In this study,twomolecular markers were identified to be linked to in coupling phase to Pm12 andPm3b by using RAPD technique.The results are showed just as follows. 1、Cross was carried out between Pm12 as resistant parent and Chancellor assusceptible parent,and F1 plants from the cross were selfed to obtain F2 mappingpopulation.

本研究利用 RAPD 分子标记技术对小麦抗白粉病基因 Pm12 和 Pm3b 进行了分子标记分析，获得的主要结果如下：1、以 Pm12 为抗病亲本，Chancellor 为感病亲本，两者杂交的 F1代通过自交获得F2分离群体。

Because of good selectivity to anions in solution of calix[4]pyrroles, the synthesis of calix[4]pyrrole-bonded stationary phase will also provide new means for separation to anions and neutral substances in chromatography and promote the development in supramolecular chemistry.

基于杯[4]吡咯化合物在溶液中对阴离子的选择性识别性能，利用其对色谱固定相进行改性以合成针对阴离子和中性小分子化合物的色谱柱将为色谱分离技术提供新的手段，推动超分子化学在分析化学中的应用并促进杯吡咯化学的发展。

When purify carries the suspension colloid grain of negative electric charge in natural raw water, it is OK that its element linear grows the positive electrical charge that contains in catenary to be opposite remove electric sex to counteract the double function that reachs adsorption to bridge at the same time, and water-solubility of starch of this kind of modified is good, burnt viscosity is stable, cast impose kind agile, it is a kind of function exceedingly good organic, inorganic and compound high polymer garrulous coagulates agent.

在去除天然原水中带负电荷的悬浮胶体颗粒时，其分子线性长链中所带有的正电荷对可以同时起到电性中和及吸附(物质表面吸住周围介质中的分子或离子现象)架桥的双重功能，而且这种改性淀粉水溶性好，糊粘度稳定，投加方式灵活，是一种性能优异的有机-无机复合高分子絮凝(使液体中悬浮微粒集聚变大或形成絮团，从而加快粒子聚沉，达到固-液分离的过程)剂。

The mechanism of molecular distillation was analyzed in this paper.

分子蒸馏是一种新型的分离技术，与传统的分离技术相比：操作温度远低于液体沸点，蒸馏压力在极高真空度下，受热时间短，系统基本绝氧，能最大限度地保证物系中的有效成分。

The molecular distillation technology differs from ordinary separate technology.

分子蒸馏技术与传统的分离方法有着本质的区别，可以达到其他分离技术无法达到的效果。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。