分子分离

NaA zeolite membranes have been found a potential application in the separation of the small-sized gas molecules ...

NaA型沸石分子筛膜在小分子气体分离和渗透汽化分离等领域预期有着良好的应用前景。

For further understanding the effect of the strain on K-bearing silicate mineral, an experiment with KO2 strain cultured in nitrogen-containing and nitrogen-free medium was conducted. By putting mineral powders into the medium and measuring the concentration of capsular polysaccharides, we concluded that the strain can accelerate the weathering rate of silicate minerals. The results showed that the polysaccharides secreted by the strain in the growth process could help the bacterial to adhere to the mineral surface effectively, and created the bacterium-mineral complex, which formed a mircro-enviorment avail the release of potassium. Another result was that there was higher level of Carbonic Anhydrase activity, which revealed that some exo-protein or enzyme produced by the bacterial had postive impact on the process of releasing potassium ion. We carried out the bacterial fermentation for larger scale production of the bacterial secretion, which was used to sperate, and identify the small molecules related to the mineral-bacterial interaction. After the analysis, it was found that the strain can produce many kinds of small molecules, such as 2-Hydroxybenzoic acid, 4-Hydroxyphenylacetic acid, methyl-4-hydroxybenzoate, etc.

分别将菌株接入含有不同矿粉的培养基中培养，检测其在有氮、无氮培养基中荚膜多糖含量的变化，并在以钾长石和黑云母为矿源的情况下，比较研究了菌株在有氮、无氮培养基中对矿物的风化能力，通过一系列的实验，证实胶质芽孢杆菌在生长过程中所分泌的粘稠胞外聚糖可帮助细菌有效黏附在矿物表面，形成细菌-矿物复合体，并改变及维持该复合体内部的微环境，有助于该菌的解钾作用，而且细菌分泌的胞外蛋白质在该菌对含钾硅酸盐矿物的解钾作用过程中发挥重要作用；将胶质芽孢杆菌接入以钾长石和黑云母为矿源的有氮、无氮培养基中培养，检测到该菌碳酸酐酶活性的变化，并进行批量发酵后小分子酸性分泌产物的提取、分离、纯化与鉴定等方面的研究，结果表明，该菌可产生2-羟基苯甲酸，4-羟基苯乙酸，4-羟基苯甲酸甲酯等小分子物质。

In chapter four, molecular imprinting polymers against amino acid derivatives utilizing 2-VP+AM as combined functional monomer and trimethylolpropane trimethacrylate as tribasic cross-linker were prepared and result was compared with those with the same amino acid derivative as the imprinted molecules utilizing ethyleneglycol dimethacrylate as dibasic cross-linker. It was found that cross-link intensity of the molecular imprinting polymer is higher when TRIM was utilized and baseline separation for the imprinted molecule could be achieved. When TRIM was utilized as cross-linker more molecule is imprinted during the polymerization.

第四章采用2-乙烯基吡啶+丙烯酰胺复合功能单体，考察了三元交联剂三甲氧基丙烷三甲基丙烯酸酯烙印氨基酸衍生物的情况，并与二元交联剂乙二醇二甲基丙烯酸酯烙印相同氨基酸衍生物的情况进行了对比，发现三甲氧基丙烷三甲基丙烯酸酯交联剂在较小的用量下就可使分子烙印聚合物达到足够的交联度，实现烙印分子的基线分离。

CD was added into running buffer in above electrophoresis system, and the separation efficiency of polycarboxylic acids was improved further by the supramolecular interaction. Based on that, the method for separation and determination of several di- and tricarboxylic acids, including oxalic, malic, tartaric and citric acids, found in fruit juices was developed, which supplied a fast, convenient and sensitive method for the food analysis, the quality control of foods and various drinks.

果汁中多元有机酸的毛细管区带电泳/Cu电极安培检测在上述分析体系的电泳介质中添加β-CD，利用CPB与β-CD的协同超分子作用，进一步提高了多元有机酸的分离效率，成功实现了水果中四种常见多元有机酸草酸、柠檬酸、苹果酸、酒石酸的基线分离，建立了几种果汁中上述有机酸毛细管区带电泳/Cu电极安培检测定量测定方法，为食品分析、食品饮料质量控制以至辨别酒类商品真伪等提供了一种方便、快速、灵敏度高的分析方法。

It has also been discovered that, before complete purification, the synthetic HWTX-Ⅰ, after oxidative treatment, showed wider and more asymmetric peaks than the native HWTX-Ⅰ that was denatured and then renatured under the same experimental conditions, which suggests that there is even greater conformational unhomogeneity in the synthetic HWTX-Ⅰ.

推测除了在合成HWTX-Ⅰ复性过程中有少数分子发生二硫键错配外，还有部分分子在固相合成中发生了消旋作用，需要交替采取多种色谱法才能完全分离纯化复性后的合成多肽产物。

The study ~s conducted to elucidate the mechanism by which the expression of adhesion molecules on human endothelial cells ~s up-regulatecL Methods Human endothelial cells derived fium umbilical venisQ-IUVEC were coincubated in viny whh native human sennn albtuiiin, HSA modified v~ith advanced glycation end products or RPMI-1640 culture medium. The expression of adhesion molecule intereellular adhesion molecule-l(7ICAM-l), vascular cell adhesion molecule-l and E-selectin was determined by irnrnunofluorescen staining and flow cytometer analysis.

分离正常人脐静脉内皮细胞，将晚期糖基化终产物修饰的人血清白蛋白、人血清白蛋白以及RPMI-1640完全培养基与HUVEC在体外共同培养，用荧光单克隆抗体染色，流式细胞仪定量检测内皮细胞表面细胞间粘附分子-1（ICAM-1）、血管细胞间粘附分子-1（VCAM-1）、E-选择素的表达。

The range of Mw values of amylose from different types of rice starch is from 3.29×105～2.75×106. There are no significant differences in the molecular weight distribution, Mw and RMS of the amylose leaches out at the temperature between the onset pasting temperature and 90℃.

研究表明，90℃以上高温不利于用浸出法从直链淀粉含量低的大米淀粉中分离直链淀粉，当温度低于90℃而高于糊化温度时，各个温度下均可用浸出法分离出直链淀粉，得到的直链淀粉具有十分相似的分子量分布、重均分子量和分子旋转半径。

The hard segments pack firmly and form hard segment domains which bear certain degree of regularity. In polyether polyurethane zwitterionomers,variation of the amount of hydrogen bondings formed between hard and soft segmentswith the degree ionization conforms with the phase separation between the hard andsoft segments observed in wide line study.

特别是聚醚聚氨酯离聚体浓溶液中软段与硬段之间形成的氢键的数量和固体变温方法测得的样品的相分离情况完全一致，表明浓溶液中高分子链段间的相互作用和固体条件下的情况一致，可以通过浓溶液的核磁共振研究来了解链段间的相互作用，研究影响体系相分离的因素。

Molecularly imprinted polymers are a new kind of macromolecular materials with molecular recognition capability. Because MIPs have the advantages of predetermination, specificity and practicability, they have been extensively used in chromatographic separation, antibody mimic, solid-phase extraction, biosensors, catalysing reaction and other relevant fields.

分子印迹聚合物是一种具有分子识别能力的新型高分子材料，由于它具有预定性、专一性和实用性等优点，因此在色谱分离、抗体摸拟、固相萃取、生物传感器及模拟酶催化等诸多领域都有广阔的应用前景。

Based on the traits and the molecular markers of Sorghum×Sudan grass, the near-isogenic lines (N1Ls) of plant height, leaf number and related molecular markers were determined from the recombinant inbred line F(subscript 6:7) family. The material could be used for the QTLs analysis.

以株高等表型性状和分子标记为基础，从高粱×苏丹草的F(下标 6：7)重组自交系群体中分离株高、叶片数2个性状及对这2个性状有效应的分子标记的近等基因系，为精确定位性状的QTL及分析QTL的作用提供有用的遗传材料。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。