分光的

Regular porosity in about 38 nanometer size was observed using atomic force microscopy. Excitation and Emission spectrum were obtained by using F-4500 fluorometer.

用F-4500型荧光分光光度计测量其三维荧光谱，与未经氧化的铝片作对比，发现前者的激发谱在280 nm~340 nm有一个较宽的谱带，并且在253.8 nm附近有一个肩峰，发射谱有一个以417.4 nm为峰值的较宽谱带，而后者却无任何明显的发射峰和激发峰。

Based on the different permeability of DNA-intercalant dyes YO-PRO-1 and propidium iodide to the membrane of viable, apoptotic and necrotic cells, cell samples were stained with 4μmol/L YP and 4μg/ml PI for 10 min, and the fluorescence intensity of both YP and PI were measured by fluorometer at Ex/Em wavelength of 485/538nm and 530/590nm, respectively.

根据正常细胞、凋亡细胞和坏死细胞的细胞膜对核酸荧光染料的不同选择通透性，用4μmol/LYO-PRO-1和4μg/ml碘化丙啶(Propidiumiodide，PI)染色96孔板中的细胞样品。分别在485/538和530/590的检测波长下借助荧光分光光度计检测细胞样品孔的YP和PI荧光强度。

This article uses the microwave oven separately to use 700W,336W processing for 3min on pretreatment of three vegetables(celery,greengrocery and green soy bean),andusing the ultrasonic wave extraction process and 70％methyl alcohol to withdraw,then Uses the spectrophotometer method to determine and compare total flavonoid content of the three vegetables in which two samplewere processed by microwave,and the other was fresh.

本文利用微波炉对新鲜的三种常食蔬菜进行处理，分别用700W，336W处理3分钟，采用超声波提取法用70％的甲醇进行提取，然后用分光光度法对三种蔬菜未经微波处理的新鲜样品和经两种微波处理的样品进行总黄酮含量的测定和比较。

Determination of hydrogen chloride in air by ion chromatography was developed, the collection of sample and conditions of determination were investigated.

用离子色谱法测定环境空气样品中的氯化氢，建立了该空气样品的氯化氢采集和离子色谱分析的条件，并将该分析方法与常用的硫氰酸汞分光光度法相比，具有操作简便、快速、灵敏度高的特点。

A spectral-correction-integral- photometry method and its application in the measurement of the luminous flux of blue LEDs are introduced.

介绍了一种光谱校正积分光度法及其在蓝光LED光通量测量中的应用，对测量中的各种不确定因素作了理论分析，并与单纯的分光光度法和积分光度法的不确定度作了比较，证明该方法可实现极高的测量精度。

For thesake of knowing mechanism of preparing nacreouspigment of mica titanium and adjusting pH with urea, we need to measure fast and exactly concentration of titanium to direct preparing top grade nacreous pigment of mica titanium.

为了更好地了解尿素中和法制备云母钛珠光颜料过程中钛水解的机理，指导高档次云母钛珠光颜料的制备，需要对该过程中钛的含量进行准确快速的测定，本文对过氧化氢分光光度法测定钛的方法进行了实验研究。

The method of using formaldehyde buffer solution a b sorption-hydrochloric pararosaniline spectrophotometry to determine sulfur diox ide in air has higher sensitivity and good selectivity,and prevents from adoptin g mercurial absorbent.But the conditions in lab are not well controlled,the v alue of reagent blank and standard curvilinear slope can't accord with the one s tipulated in"Methods for Air and Waste Gas Monitoring and Analysis".Some opra tional techniques for SO2 determination in lab are introduced for reference.

文摘：以甲醛缓冲溶液吸收-盐酸副玫瑰苯胺分光光度法测定空气中的二氧化硫，方法灵敏度高，选择性好，避免了使用含汞的吸收液，但如果实验条件控制不好，试剂空白值及标准曲线的斜率就会不符合《空气和废气监测分析方法》中规定的值，本文着重从操作技巧方面对二气化硫测定的实验室质量控制进行分析，仅供参考。

In this paper the thymus DNA of calf and the pBs plasmid are the main objects of our studies in ultraweak luminescence. By means of PIAS(Photon-counting image acquisition), ultraweak luminescence detector and Lambda Bio 40 of UV/VIS spectrophotometer, we studied the UL of DNA induced by the three oxygen free radicals: singlet oxygen Oa, hydroxy radical and superoxide anion(O2 ). We found that the OH can greatly increase the UL intensity of DNA.

本论文以小牛胸腺DNA和质粒pBs为实验材料，利用光子图像探测系统(Photon-counting image acquistion，PIAS)、微弱发光测量仪和UV／VIS分光光度计和生物统计学的成组比较法原理对单线态氧(~1O_2)、羟自由基、超氧阴离子(O_2~-)三种氧自由基诱导DNA超弱发光的变化过程进行了研究，结果发现这三种氧自由基中·OH能诱导DNA的超弱发光发生显著的增强变化，而其他两种氧自由基对DNA的超弱发光并无显著诱导作用。

An innovative principle and scheme on static large field of view polarizationinterference imaging spectrometerbased on the field of view compensatedSavart polariscope is put forward on the basis of USPIIS,by means of field of viewcompensation.

在USPIIS的基础上，采用视场补偿方法，提出了基于视场补偿型Savart偏光镜的稳态大视场偏振干涉成像光谱仪的创新原理及方案；论证了SLPIIS的分光机理、成像及广角补偿原理。4 USPIIS、SLPIIS调制度及通量的分析与研究

By examining RLS spectral features of four systems including Al3+-DNA, azoviolet-cationic surfactants, fast red VR-proteins, ponceau G-proteins in this thesis, and proved that the instrumental conditions of spectrofluorometer have strong effect on the shape of resonance light scattering spectra.

本文在讨论Al~(3+)-DNA、镁试剂Ⅰ阳离子表面活性剂、固红VR-蛋白质、丽春红G-蛋白质四个体系的RLS光谱特征的基础上，证明荧光分光光度计的仪器条件和样品的分子吸收等因素会影响共振光散射光谱的形状，降低检测的灵敏度。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失