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分光光度计

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Instrumentation IR spectra were measured on a Perkin–Elmer 16 PC FT-IR spectrophotometer.

仪器仪表红外光谱测定的扩增16 电脑红外分光光度计

From the results obtained in the study, the following conclusions can be made:The methylene blue and spectrophotometer method can capture the free radicals generated successfully. This is an effective way to measuring the free radicals quantitatively in cavitation, moreover it is easy to fulfill.The intensity of cavitation increases with reduction in the cavitation number. But the different mechanism of various enhancive processes (chemical and physical processes) results in different effect by cavitation number.The effect of operating parameters, such as inlet pressure, temperature and time on cavitation indicate that optimum operating conditions exist at the cavitation enhancive effect is maximum.It is important to optimize the structure of hydrodynamic cavitation reactor for achieving a desired effect. A reasonable geometric parameter can promote the enhancive effect for a fixed hydrodynamic condition.Ventilating appropriate gas to cavitating flow can increase cavitational intensity and hence promote the enhancive effect of hydrodynamic cavitation.

研究结果表明:亚甲基蓝分光光度计法,能够成功地捕捉到水力空化产生的羟自由基,是定量检测空化自由基简便易行的有效方法;空化程度随空化数的减小而增强,但对于不同的强化过程,因其强化机理不同,故空化数对强化效应的影响也不同;水力空化系统的压力、温度及时间等操作参数对空化强化效应的影响规律表明:在适宜的操作条件下可产生最佳的空化强化效果;空化发生器结构的优化设计是保证空化强化效应的重要前提,在一定的水力学条件下,合理的结构尺寸可以增强空化作用效果:往水流中适量加入干扰气体能够提高空化强度,增强空化强化效果。

Determination of the equilibrium constant of a coordinated compound.

四、铁离子与磺基水扬酸配合物的组成和标准稳定常数的测定,了解用比色法测定配合物的组成和配离子稳定常数的原理和方法,掌握分光光度计的使用及有关试验数据的处理方法。

Anthocyanidins in Euphorbia pulcherrima were extracted and identified by use of UV-vis spectrophotometry, high performance liquid chromatography and mass spectrometry.

用紫外-可见光分光光度计、高效液相色谱和质谱技术对一品红红色苞片中的花色素提取液进行了初步鉴定。

After acquiring the ultraviolet absorption spectrum and its first order derivative spectrum distributions of Avermectin B1 by using UV-2401 PC spectrophotometer and spectrum-measuring system,we found that coordinates of the peak A252.2 and the valley A256.8 are not disturbed by the different forms of preparations.

用UV-2401 PC紫外分光光度计及光谱工作站,以1 nm狭缝宽度,2 nm Δλ间隔,在190~400 nm波长范围内快速扫描采集阿维菌素B1紫外吸收光谱,拟合一阶导数光谱发现峰顶A252.2,和峰谷A256.8在波长坐标上的位置不受不同制剂干扰。

Result show:the solution,extracted from rat rail tendons,consist mainly of type I collagen;No growth of bacteria in 37℃ thermostatic culture box for 72 hours;Absorbing the height of ultraviolet-spectrophotometry is 230 nm by using flicker photometer examination;Amino acid in type I collagen consist of aminoacetic acid(40 per cent) and proline(14.3 per cent);Under microscope:collagen gel netted constraction consist of original collagen fibril that had been fairly well-distributed and had a quite regular striate lines ;The collagen solution prepared collagen gel matrixes in 37℃ thermostasis for culture cells and also prepared collagen solid film by washing collagen gel with a phosphate solution for plasty surgery materials.

结果:提取液主要为Ⅰ型胶原蛋白;72小时37℃恒温培养箱内培养无细菌生长;紫外分光光度计测定,吸收高峰为230nm;氨基酸成分主要为甘氨酸:占40%,脯氨酸:14.3%。显微镜下见:相互交织的胶原原纤维结构组成的网状结构;纤维均匀,有规则横纹。胶原蛋白液在37℃恒温下,可制成凝胶,用于培养细胞;用磷酸液冲洗胶原蛋白凝胶可成固体片状,制成胶原膜,用作医用材料。

Methods BSA incubated withglucose were subjected to PAGE and SDS-PAGE,fluorescence spectrum and fluorescence intensity were measured using fluorometer.

牛血清白蛋白与葡萄糖在体外共同孵育后,经聚丙烯酰胺凝胶电泳及荧光分光光度计测定荧光值和扫描荧光光谱。

The chemosensitivity, apoptosis and caspase3 activity of K562 and K562/A02 cells were evaluated by MTT assay, flow cytometer and fluorometer respectively.

以MTT、流式细胞术和荧光分光光度计观察RPL6对化疗药物耐药性、凋亡和caspase3的作用。

The chemosensitivity, apoptosis and caspase??3 activity of K562 and K562/A02 cells were evaluated by MTT assay, flow cytometer and fluorometer respectively.

以MTT、流式细胞术和荧光分光光度计观察RPL6对化疗药物耐药性、凋亡和caspase??

Regular porosity in about 38 nanometer size was observed using atomic force microscopy. Excitation and Emission spectrum were obtained by using F-4500 fluorometer.

用F-4500型荧光分光光度计测量其三维荧光谱,与未经氧化的铝片作对比,发现前者的激发谱在280 nm~340 nm有一个较宽的谱带,并且在253.8 nm附近有一个肩峰,发射谱有一个以417.4 nm为峰值的较宽谱带,而后者却无任何明显的发射峰和激发峰。

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