分光光度计

Methods 100 mg of muscle of rat corpse was preprocessed before trimethylamine was extracted with anhydro-methylbenzene, then trinitrophenol was added into extracts, and yellow compounds emerged. The optical density was detected by spectrophotometer at 410 nm and the concentration of TMA-N was calculated using the standard curve. Results There was good linear correlation (R^2=0.9991, P.001) between OD and the concentration of TMA-N in the range of 1~10 mg/L.

大鼠肌肉组织样本100 mg经预处理后，将三甲胺抽提于无水甲苯中，与苦味酸作用，形成黄色的苦味酸三甲胺盐，用分光光度计在410 nm处检测吸光度，标准曲线法计算TMA-N含量。

The concentration of unoxidized AA remaining in solution was measured with the CUPRAC (cupric ion reducing antioxidant capacity) spectrophotometric method without interference from the amino acids tested.

在溶液中未氧化的抗坏血酸之浓度，可以分光光度计方法测得氨基酸之CUPRAC。

DNA extraction quality of Venturia inaequalis using CTAB method,SDS method,Parker's method and modified SDS method was detected by ultraviolet spectrometer.

采用CTAB法、SDS法、Parker法及改进的SDS法提取苹果黑星病菌的DNA，经紫外分光光度计测定，改进SDS法提取的DNA得率较其他3种方法高，约为Parker法的2倍。

In order to determine the contents of Vitamin C,Nitrate,and Nitrite in varied Zingiber officinale and evaluate the quality of Zingiber officinale,ultraviolet spectrophotometer is used to measure the absorbency of Zingiber officinale at special wavelength.

为评价不同处理方法对生姜中V itC、硝酸盐和亚硝酸盐含量的影响，通过紫外吸收分光光度计在特定波长下的吸收值分别对鲜姜、煮姜和泡姜进行测定。

The sizes of the self-assembly micelles of the amphiphilic block copolymers and the effects werestudied by dynamic light scattering and UV-visible spectrophotometer. The micelle sizes werelargely influenced bythe hydrophobic chain content in the copolymer, the properties of the solvent and the copolymer concentration in the organic phase. The morphology of the micelles was investigated by transmission electron microscopy. Theresults showed that the micelles appeared spheres with inner core and outer shell. The critical association concentrations were determined by pyrene monomer fluorescence probe technology. The degradability of copolymer was studied by the loss of the intrinsic viscosity in the degradation process. The results indicated that the degradation rate of PECL was slower thanthat of PELLA and PEDLLA. The stability of the copolymer micelle dispersion was examined by measuring the critical flocculation concentration, which gradually decreased with increasing the content of thehydrophobic chain in the copolymer. The rheological results showed thatthe viscosity ofthe micelle dispersion with higher content of PEG segments was firstly decreased and then increased with the increasingthe temperature. Otherwise, the variation of viscosity was irregular. The viscosity of the micelle dispersion increased with the increase of the electrolyte (Na 2SO 4) concentrations. Polymer micelles paclitaxel was prepared by self-emulsification/solvent evaporation method and solid dispersion technique with amphiphilic block polymers as the carrier material.

运用动态光散射和紫外分光光度计研究了两亲性嵌段共聚物自组装胶束的粒径及其影响因素，结果表明，自组装胶束的形成机理受制备方式的控制，胶束的粒径随着共聚物相对分子质量或疏水嵌段相对分子质量和有机相中共聚物浓度的增大而增大，随着有机溶剂的水溶性的增强而减小；采用透射电镜观察胶束的形态结构，发现胶束呈具有核壳结构的均匀球形；采用芘荧光探针法测两亲性嵌段共聚物的临界聚集浓度；通过降解过程中共聚物特性粘度的变化研究两亲性共聚物的降解性能，发现与PELLA和PEDLLA的相比，PECL 降解速率较慢；随着共聚物中疏水嵌段含量的增大，纳米分散液的稳定性逐渐下降；PEG含量高的PEDLLA纳米分散液随着温度的升高，纳米分散液的粘度先下降然后增大，反之，没有规律性，且随着电解质Na 2SO 4浓度的增大，体系粘度增大。

Extracted DNA by CTAB, in proportion to the additions of PVP and β-mercap toethanol to the extraction buffer were capable of preventing browning from taking place in the extraction, Tris-Phenol (Stabilized with 0.1M Tris, pH 8.0)/Chloroform/isoamyl alcohol were used to remove protein and impurity in the second extraction, during crude DNA used to Isopropanol DNA and deterged by DNA detergent, without using RNase remove RNA, we detected the quality and concentration of DNA respectively for normal leaves and drought stress leaves by Agarose gel electrophoresis of genomic DNA and ultraviolet spectrometry photo.

用20%聚乙二醇(polyethylene glycol，PEG6000)模拟干旱，采取CTAB提取缓冲液，按一定比例加入PVP和β-巯基乙醇，用Tris平衡酚－氯仿－异戊醇除去蛋白质及杂质，在粗提时用异丙醇沉淀DNA，并用DNA洗液（10mmol/L醋酸铵，75%乙醇）洗涤DNA，在纯化时用乙醇沉淀DNA，且没有加入RNase去除RNA的方法，分别提取2个品种正常和干旱胁迫的苹果属叶片总DNA，并对提取的DNA进行琼脂糖凝胶电泳和紫外分光光度计检测。

Finally, the properties of the drug-loaded SN have been evaluated with the help of UV spectrophotometer, and with the small molecular and water insoluble drug-sulpiride as a model drug. It is found that the entrapping efficiency of drug-loaded SN in entrapping method can amount to 96.87% and that in adsorption method is 87.63%, which show that the loading mechanism of SN is mainly adsorptive mechanism.

最后，利用紫外分光光度计，以疏水性小分子药物舒必利为模型药物，对所制备的淀粉纳米粒的载药性能进行了评价，发现淀粉纳米粒包埋载药的包封率可达到96.87％，吸附载药的包封率达到87.63％，淀粉纳米粒对舒必利的载药机理主要是吸附机理。

Current study compared the effects of the method of the low temperature storage without sterilization and preservatives with the method of sterilization under 80 ℃ for 15 min and then room temperature storage with potassium sobital acid on the gingers' flavor quality, color, nutrient and microorganism etc.

该试验对保存的泡姜进行了感官评价；采用分光光度计法对泡姜中的Vc、蛋白质、可溶性总糖和姜辣素进行检测分析；用高效液相色谱检测有机酸；采用稀释平板法对泡姜中的微生物进行纯化分离。

NOTE—Either absorbance or transmittance measurement may be used for preparing inocula.

在一个相当狭窄的频率波段中测量透光率要求一个适合的分光光度计，其光源的波长可以改变或者限制通过使用580nm或者530nm的过滤器来读取在管试验中。

At 10 minutes before the injection of mustard oil, an Evans Blue solution was administrated by a jugular vein injection to climacteric rat. After scarifying the rat, the hind paw was removed for measurement of the Evans Blue excretion was measured by a spectrophotometer to define the inflammation-induced plasma extravasation.

在芥末油注射前十分钟将伊氏蓝溶液经由颈静脉注射进入大白鼠体内，俟大白鼠牺牲后取下其后脚掌，使用分光光度计测量脚掌组织的伊氏蓝渗出量来定量发炎所导致血浆外渗之程度。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。