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分光光度计

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The polysaccharose of mycelium of Grifola umbellate (PPS1) and the polysaccha rose of sclerotium of Grifola umbellate (PPS2) both were extracted by hot water leaching way, which were purified dispoing by Sevage way, determined the polysac charose content by 3,5-dinitro-salicylate way, and comparablly analysed infrared spectrum by Fourier transforming infrared spectrophotometer,which suggest that two kinds of polysaccharose were the similar infrared spectrum.

本试验通过人工液体浅层培养方式成功培养出猪苓菌丝体;经热水浸提法分别提取猪苓菌丝体多糖(PPS1)和猪苓菌核多糖(PPS2);用Sevage法进行纯化处理;用3,5-二硝基水杨酸法测定其糖含量;用傅里叶变换红外分光光度计对两种多糖进行红外光谱比对分析,结果表明两者红外光谱相似。

In particularly, we conducted a deep discussion on potassic testing conditions using the TAS-986 model atomic absorbing spectrophotometer, with a flaming (air - acetylene ) approach.

尤其是对TAS—986型原子吸收分光光度计用火焰法测钾的条件作了深入的探讨。

The biological effect of pUCP18/lasR was detected by RT-PCR, NAD method and the assay of pyocyanin.

RT-PCR检测LasB基因和LasI基因mRNA的表达,NAD法测定外毒素A的活性,紫外分光光度计测定绿脓菌素的产生水平。

The result indicates that the best method for extracting β-carotene is to saponify the test specimen one hour with 4% NaOH methyl alcohol solution in 60-70'c and then to separate with 3:7 acetone-benzine component solvent as extraction solvent, MgO as the absorbent, the acetone-benzine as the eluent. Extracted solvent has been determined in the color dish condition (1cm) by ultraviolet spectrophotometer at 447 nm. The result shows that the β-carotene in carrotis 0.134 mgg^(-1), 0.0863mgg^(-1) in red pepper and 0.0426 mgg^(-1) in spinach.

结果表明,比较优先的β-胡萝卜素提取方法是:试样用4% NaOH甲醇溶液在60℃~70℃皂化1h,用3:7的丙酮-石油醚混合溶剂提取,以Mgo为吸附剂,丙酮-石油醚为洗脱剂分离,提取液用紫外分光光度计在波长447nm,1cm比色皿条件下测定,得出蔬菜中β-胡萝卜素含量:胡萝卜为0.134mgg^(-1),红椒为0.0863mgg^(-1),菠菜为0.426mgg^(-1)。

After spraying the fermentation supernatant of endophytic actinomycetes GKSHJA and PR1-8, the impact of the endophytic actinomycetes against Sphaerotheca fuliginea histomorphology were tested by the differential interference, and the enzyme activity of POD, SOD, PAL on cucumber leaf treated by spraying endophytic actinomycetes were tested by the spectrophotometer.

对喷施内生放线菌GKSHJA及PR1-8发酵上清液处理后采用微分干涉的方法观测生防菌对白粉菌的组织形态学影响,利用分光光度计测定喷施发酵液后黄瓜叶片内POD、SOD、PAL酶活变化。

Methods: The culture mediums in 1% SBOS, 0.36% stachyose, 0.52% sucrose and 0.11% raffinose were prepared respectively, then Bifidobacteria and Enterobacillus were inocuted into the mediums.

按1%大豆低聚糖、0.36%水苏糖、0.52%蔗糖、0.11%棉子糖含量配制培养液,分别接种各试验菌株,在0、12、24小时测定各培养液吸光度A值(分光光度计,550nm)和pH值。

This experiment which adopting technology of examination of muscle, utilizing modern organized chemical method, using scanning photometer, doing a eight-week staminal training towards six ordinary examinee, analyses the samples of the vastus lateralis of the right leg before and after the experiment.

采用肌肉活检技术,运用现代组织化学方法,结合扫描显微分光光度计定量的方法,对6名普通受试者进行8周耐力跑训练。

N-substituded thioacridones with five different structures were synthesized via a route involving Ull-man reaction, cyclization, N-alkylation and sulfurization. The structures of each thioacridone were identified with IR and MS. The results from UV-spectrophotometry show that the five N-alkythioacridones all have stronger absorption in the visible light region.

采用乌尔曼反应、酸催化环化、N-烷基化和硫代化四步反应路线合成了5种不同结构的N-取代硫代吖啶酮,利用IR和MS分析手段表征了其结构,UV分光光度计的测定结果表明5种硫代吖啶酮在可见光区都有较强的吸收。

The stability of nano-alumina aqueous dispersion is tokened by spectrophotometer and particle size distribution instrument. It is proved that when condition is dispersant PMAA =0.4%, pH =9, nano-γ-alumina content = 4%,ultrasonic for twenty minites, excellent stable nano-alumina aqueous dispersion can be acquired.

分光光度计和粒度分析仪表征了纳米粒子水分散液的稳定性,结果表明纳米γ相Al2O3浓度为4%,分散剂为阴离子聚电解质,用量0.4%,PH=9,采用超声波分散20分钟得到最好的分散效果,纳米颗粒的平均粒径为205nm。

The optical density value of cells was detected by MTT colorimetric method, content of MDA and NO in the supernatant fluid of cultured cells was detected by spectrophotometer, and the transudatory content of LDH was detected by automatic biochemistry analyzer.

用MTT法检测细胞吸光度值,利用分光光度计分别测量细胞上清液中的MDA和NO含量。用自动生化分析仪测定培养液中LDH漏出量。

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