分光光度计

A basic knowledge of microbiology, will be detected with a microscope observation of micro-organisms, medium Preparation, disinfection and sterilization, separation and purification of micro-organisms, bacteria counting method A chemical analysis of the theoretical knowledge and can use chemical analysis methods and equipment of the components of material analysis Master fermentation process principles, understanding of beer, liquor, fermentation production technology Master protein related knowledge, PAGE gel electrophoresis technology, high-speed centrifugal Genetic engineering master the basic theory of knowledge, the extraction and purification of genes, PCR principle, gene recombination technology Master of the basic theoretical knowledge, the immobilized enzyme, the enzyme preparation Application Master of animal and plant tissue culture techniques of experimental steps studied experimental apparatus: Balance (scales, electronic scales, Analytical Balance), or acidity, spectrophotometer, liquid gas chromatography, acid-base titration apparatus, microscopes, centrifuges, ultrasonic cleaning, constant temperature oscillation incubator, super-clean table, steam sterilization pot, the pot constant temperature water bath, PAGE device apparatus, oven, fermenter, such as PCR instrument.

具备微生物理论基础知识，会用显微镜检测观察微生物，培养基的制备、消毒与灭菌，分离与纯化微生物，细菌的计数法具备分析化学的理论知识，能运用化学分析和仪器分析方法对物质的组分进行分析掌握发酵工艺原理，了解啤酒、白酒、发酵生产工艺掌握蛋白质相关知识，PAGE凝胶电泳技术，高速离心掌握基因工程的基本理论知识，基因的提取和纯化，PCR技术原理，基因重组技术掌握酶的基本理论知识，酶的固定化方法，酶制剂的应用掌握动植物组织培养技术的实验步骤学过的实验仪器：天平（台天平，电子天平，分析天平）、酸度计、分光光度计、液气相色谱仪，酸碱滴定仪器，显微镜，离心机，超声波清洗器、恒温振荡培养箱、超净工作台，蒸气灭菌锅、恒温水浴锅，PAGE电泳装置仪器、干燥箱、发酵罐、PCR扩增仪等。

The property of Rhodamine B was examined in hydrochloric and Trifluoromethyl sulfonic acid, and the standard working curves in thin polymer film were set up.

用紫外分光光度计分别测量盐酸、三氟甲磺酸在不同浓度时罗丹明B特征吸收的变化，选取酸浓度与罗丹明的特征吸收的变化量呈线性关系的线段部分作为标准工作曲线。

Using thymol -sodium hypobromite as chromogenic reagent,and extracting the colour formed with butanol,a spectrophotometric method for the determination of arginine in fermentation broth was studied.

以百里酚的次溴酸钠溶液为显色剂，用正丁醇萃取显色产物，以分光光度计比色测定有机相的吸光度值。

The relationship among stray light,absorbance error and absorbance true value for UVVISS was discussed in this paper.

本文研究了紫外可见分光光度计UVVISS的杂散光S.L.Strary Light与吸光度误差和吸光度真值之间的关系导出了S.L。

The relationship among stray light,absorbance error and absorbance true value for UV/VISS was discussed in this paper.

本文研究了紫外可见分光光度计的杂散光 S.L。与吸光度误差和吸光度真值之间的关系；导出了S.L。

It consists of a tungsten lamp, amonochromator, an optical fiber, a shielding chamber, a photomultiplier tube, a reading meter,a high voltage supply source.

该仪器是在对国产72G型分光光度计改装基础上研制而成的，仪器由钨灯、单色器、双臂光纤、暗盒、光电倍增管检测器、读数装置、高压电源等部分组成。

It was studied that Apolipoproteins AⅠand B were determined by scattering immunoturbidimetric method using fluorescence spectrophotometer in stead of laser turbidimeter,and meanwhile the reference values of apolipoproteins AⅠand B of health persons were investigated.

研究了荧光分光光度计的免疫散射比浊法代替激光散射浊度仪来测定载脂蛋白AⅠ和载脂蛋白B，同时测定正常人的ApoAⅠ和ApoB的参考值。

Our lab has many types of equipment, such as full-size ultracentrifuge, benchtop refrigerated ultracentrifuge, gel documentation system, ice machine, MILLIPORE, PCR thermocycler, autoclave, ultra freezer, UV/visible spectrophotometer, UV transilluminator, ultrasonic cleaner, culture shaker, stir plate, low-speed and full size centrifuge, automatic Kjeldahl distillation, gas chromatograph, HPLC system, fluorescence microscope, fatex, gliding microtome.

仪器设备包括生物育种用的高速冷冻离心机、台式冷冻离心机、凝胶成像分析系统、制冰机、超纯水制备系统、PCR仪、高压灭菌器、超低温冰箱、紫外可见分光光度计、紫外观测仪、超声波清洗、恒温摇床、磁力搅拌器、低速大容量离心机、全自动凯氏定氮仪、气相色谱仪、液相色谱仪、荧光显微镜、脂肪测定仪，冷冻切片机等40余件。

Aim: To determine whether activation of κ-opioid receptor with U50,4 88 H, a selective κ-opioid receptor agonist, produces any changes in electrical u ncoupling during prolonged ischemia and whether these changes in electrical unco upling is associated with the cardioprotection induced by κ-opioid receptor ac tivation, and to explore the possible mechanism.

测量指标：以分光光度计在490nm波长下测定氯化三苯基四氯唑与活细胞反应的产物formazan含量的方法测定心肌细胞活性、测定冠脉流出液中乳酸脱氢酶的含量以及心室内压；②全心停灌70min，应用四电极法观察不同浓度U50，488H、nor-BNI和5-HD对缺血期间心肌整体阻抗和电脱耦联参数(电脱耦联时间、平台时间、电脱耦联最大速率和阻抗倍数)的影响。

This showed that the main peak position of the transmission light is between 530-540 nm, and the color saturation is about 80%. The dense green color of the uvarovite sample is caused by its higher color saturation. The theoretical analysis of the mechanism of the green coloring of uvarovite indicates that the transition of 3d-orbital electron in the Cr(superscript 3+) is responsible for the green color.

采用紫外－可见光分光光度计对样品进行吸收光谱测试，对其颜色指数进行了定量分析，所得样品颜色的主波长为530~540 nm，饱和度为80%左右，进而分析了钙铬榴石的颜色主要由Cr(上标 3+)引起，其呈色机理归因于Cr(上标 3+)的d轨道电子跃迁。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。